Apoptosis of thymocytes related to cytokine expression in experimental classical swine fever
Atrophy of the thymic cortex and loss of thymocytes were studied in 32 pigs inoculated with the virulent strain "Alfort" of classical swine fever (CSF) virus and killed at intervals from 2 to 15 days after infection. Immunohistochemical, ultrastructural, ELISA and TUNEL methods were used. The results suggested that direct action of CSF virus on thymocytes played no more than a minor role. The massive lymphoid depletion observed in the thymus, may, however, have been associated with the numerical increase in monocytes-macrophages in this organ, and their secretory activation, leading to synthesis and release of tumour necrosis factor (TNF)-α, interleukin (IL)-1α and C1q complement component as main chemical mediators, and IL-1β and IL-6 as minor mediators. These cytokines (TNF-α and IL-1α) may have played a role in the apoptosis of thymocytes, demonstrated by TUNEL and ultrastructural methods. The pathogenetic mechanism outlined may contribute to the lymphoid depletion observed in others organs in CSF and may explain the lymphopenia characteristic of the disease. © 2002 Elsevier Science Ltd. All rights reserved.
Main Authors: | , , , , , , |
---|---|
Format: | journal article biblioteca |
Language: | English |
Published: |
Elsevier
2002
|
Online Access: | http://hdl.handle.net/20.500.12792/4087 http://hdl.handle.net/10261/294549 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-inia-es-10261-294549 |
---|---|
record_format |
koha |
spelling |
dig-inia-es-10261-2945492023-02-20T10:39:46Z Apoptosis of thymocytes related to cytokine expression in experimental classical swine fever Sánchez-Cordón, P. J. Romanini, S. Salguero, F. J. Núñez, A. Bautista, M. J. Jover, A. Gómez-Villamandos, J. C. Atrophy of the thymic cortex and loss of thymocytes were studied in 32 pigs inoculated with the virulent strain "Alfort" of classical swine fever (CSF) virus and killed at intervals from 2 to 15 days after infection. Immunohistochemical, ultrastructural, ELISA and TUNEL methods were used. The results suggested that direct action of CSF virus on thymocytes played no more than a minor role. The massive lymphoid depletion observed in the thymus, may, however, have been associated with the numerical increase in monocytes-macrophages in this organ, and their secretory activation, leading to synthesis and release of tumour necrosis factor (TNF)-α, interleukin (IL)-1α and C1q complement component as main chemical mediators, and IL-1β and IL-6 as minor mediators. These cytokines (TNF-α and IL-1α) may have played a role in the apoptosis of thymocytes, demonstrated by TUNEL and ultrastructural methods. The pathogenetic mechanism outlined may contribute to the lymphoid depletion observed in others organs in CSF and may explain the lymphopenia characteristic of the disease. © 2002 Elsevier Science Ltd. All rights reserved. 2023-02-20T10:39:46Z 2023-02-20T10:39:46Z 2002 journal article Journal of Comparative Pathology 127: 239-248 (2002) 0021-9975 http://hdl.handle.net/20.500.12792/4087 http://hdl.handle.net/10261/294549 10.1053/jcpa.2002.0587 1532-3129 en none Elsevier |
institution |
INIA ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-inia-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del INIA España |
language |
English |
description |
Atrophy of the thymic cortex and loss of thymocytes were studied in 32 pigs inoculated with the virulent strain "Alfort" of classical swine fever (CSF) virus and killed at intervals from 2 to 15 days after infection. Immunohistochemical, ultrastructural, ELISA and TUNEL methods were used. The results suggested that direct action of CSF virus on thymocytes played no more than a minor role. The massive lymphoid depletion observed in the thymus, may, however, have been associated with the numerical increase in monocytes-macrophages in this organ, and their secretory activation, leading to synthesis and release of tumour necrosis factor (TNF)-α, interleukin (IL)-1α and C1q complement component as main chemical mediators, and IL-1β and IL-6 as minor mediators. These cytokines (TNF-α and IL-1α) may have played a role in the apoptosis of thymocytes, demonstrated by TUNEL and ultrastructural methods. The pathogenetic mechanism outlined may contribute to the lymphoid depletion observed in others organs in CSF and may explain the lymphopenia characteristic of the disease. © 2002 Elsevier Science Ltd. All rights reserved. |
format |
journal article |
author |
Sánchez-Cordón, P. J. Romanini, S. Salguero, F. J. Núñez, A. Bautista, M. J. Jover, A. Gómez-Villamandos, J. C. |
spellingShingle |
Sánchez-Cordón, P. J. Romanini, S. Salguero, F. J. Núñez, A. Bautista, M. J. Jover, A. Gómez-Villamandos, J. C. Apoptosis of thymocytes related to cytokine expression in experimental classical swine fever |
author_facet |
Sánchez-Cordón, P. J. Romanini, S. Salguero, F. J. Núñez, A. Bautista, M. J. Jover, A. Gómez-Villamandos, J. C. |
author_sort |
Sánchez-Cordón, P. J. |
title |
Apoptosis of thymocytes related to cytokine expression in experimental classical swine fever |
title_short |
Apoptosis of thymocytes related to cytokine expression in experimental classical swine fever |
title_full |
Apoptosis of thymocytes related to cytokine expression in experimental classical swine fever |
title_fullStr |
Apoptosis of thymocytes related to cytokine expression in experimental classical swine fever |
title_full_unstemmed |
Apoptosis of thymocytes related to cytokine expression in experimental classical swine fever |
title_sort |
apoptosis of thymocytes related to cytokine expression in experimental classical swine fever |
publisher |
Elsevier |
publishDate |
2002 |
url |
http://hdl.handle.net/20.500.12792/4087 http://hdl.handle.net/10261/294549 |
work_keys_str_mv |
AT sanchezcordonpj apoptosisofthymocytesrelatedtocytokineexpressioninexperimentalclassicalswinefever AT romaninis apoptosisofthymocytesrelatedtocytokineexpressioninexperimentalclassicalswinefever AT salguerofj apoptosisofthymocytesrelatedtocytokineexpressioninexperimentalclassicalswinefever AT nuneza apoptosisofthymocytesrelatedtocytokineexpressioninexperimentalclassicalswinefever AT bautistamj apoptosisofthymocytesrelatedtocytokineexpressioninexperimentalclassicalswinefever AT jovera apoptosisofthymocytesrelatedtocytokineexpressioninexperimentalclassicalswinefever AT gomezvillamandosjc apoptosisofthymocytesrelatedtocytokineexpressioninexperimentalclassicalswinefever |
_version_ |
1767603629739474944 |