Isolation and molecular characterization of the Arabidopsis TPS1 gene, encoding trehalose-6-phosphate synthase
An Arabidopsis thailana cDNA clone, AtTPS1, that encodes a trehalose-6-phospheta synthase was isolated. The identity of this protein is supported by both structural and functional evidence. On one hand, the predicted sequence of the protein encoded by AtTPS1 showed a high degree of similarity with trehalose-6-phosphate syntheses of different organisms. On the other hand, expression of the AtTPS1 cDNA in the yeast tps1 mutant restored its ability to synthesize trehalose and suppressed its growth defect related to the lack of trehalose-6-phosphate. Genomic organization and expression analyses suggest that AtTPS1 is a single-copy gens and is expressed constitutively at very low levels.
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Wiley
1998
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dig-inia-es-10261-2945112023-02-20T10:39:26Z Isolation and molecular characterization of the Arabidopsis TPS1 gene, encoding trehalose-6-phosphate synthase Blázquez, M. A. Santos, E. Flores, C. L. Martínez-Zapater, J. M. Salinas, J. Gancedo, C. An Arabidopsis thailana cDNA clone, AtTPS1, that encodes a trehalose-6-phospheta synthase was isolated. The identity of this protein is supported by both structural and functional evidence. On one hand, the predicted sequence of the protein encoded by AtTPS1 showed a high degree of similarity with trehalose-6-phosphate syntheses of different organisms. On the other hand, expression of the AtTPS1 cDNA in the yeast tps1 mutant restored its ability to synthesize trehalose and suppressed its growth defect related to the lack of trehalose-6-phosphate. Genomic organization and expression analyses suggest that AtTPS1 is a single-copy gens and is expressed constitutively at very low levels. 2023-02-20T10:39:26Z 2023-02-20T10:39:26Z 1998 journal article Plant Journal 13(5): 685-689 (1998) 0960-7412 http://hdl.handle.net/20.500.12792/3996 http://hdl.handle.net/10261/294511 10.1046/j.1365-313X.1998.00063.x 1365-313X en none Wiley |
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An Arabidopsis thailana cDNA clone, AtTPS1, that encodes a trehalose-6-phospheta synthase was isolated. The identity of this protein is supported by both structural and functional evidence. On one hand, the predicted sequence of the protein encoded by AtTPS1 showed a high degree of similarity with trehalose-6-phosphate syntheses of different organisms. On the other hand, expression of the AtTPS1 cDNA in the yeast tps1 mutant restored its ability to synthesize trehalose and suppressed its growth defect related to the lack of trehalose-6-phosphate. Genomic organization and expression analyses suggest that AtTPS1 is a single-copy gens and is expressed constitutively at very low levels. |
| format |
journal article |
| author |
Blázquez, M. A. Santos, E. Flores, C. L. Martínez-Zapater, J. M. Salinas, J. Gancedo, C. |
| spellingShingle |
Blázquez, M. A. Santos, E. Flores, C. L. Martínez-Zapater, J. M. Salinas, J. Gancedo, C. Isolation and molecular characterization of the Arabidopsis TPS1 gene, encoding trehalose-6-phosphate synthase |
| author_facet |
Blázquez, M. A. Santos, E. Flores, C. L. Martínez-Zapater, J. M. Salinas, J. Gancedo, C. |
| author_sort |
Blázquez, M. A. |
| title |
Isolation and molecular characterization of the Arabidopsis TPS1 gene, encoding trehalose-6-phosphate synthase |
| title_short |
Isolation and molecular characterization of the Arabidopsis TPS1 gene, encoding trehalose-6-phosphate synthase |
| title_full |
Isolation and molecular characterization of the Arabidopsis TPS1 gene, encoding trehalose-6-phosphate synthase |
| title_fullStr |
Isolation and molecular characterization of the Arabidopsis TPS1 gene, encoding trehalose-6-phosphate synthase |
| title_full_unstemmed |
Isolation and molecular characterization of the Arabidopsis TPS1 gene, encoding trehalose-6-phosphate synthase |
| title_sort |
isolation and molecular characterization of the arabidopsis tps1 gene, encoding trehalose-6-phosphate synthase |
| publisher |
Wiley |
| publishDate |
1998 |
| url |
http://hdl.handle.net/20.500.12792/3996 http://hdl.handle.net/10261/294511 |
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