Catalytic RNase P RNA from Synechocystis sp. cleaves the hepatitis C virus RNA near the AUG start codon

Previously, we described two RNA structural motifs in the hepatitis C viral (HCV) genome that can be processed in vitro by human ribonuclease P (RNase P) enzyme [J. Biol. Chem. 277 (2002) 30606]. One of these structures is located in the internal ribosome entry site and is conserved in the related animal pestiviruses [J. Biol. Chem. 278 (2003) 26844]. Here, we tested two prokaryotic RNase P ribozymes (P RNA) against this conserved structural motif. In vitro experiments indicated that P RNA from Synechocystis sp. can specifically process the viral transcript preparations in a position close to the human RNase P cleavage site. This provides additional support for the presence of an RNA structure similar to tRNA near the AUG start codon and suggests that Synechocystis P RNA may be an active agent for HCV antigenomic interventions. © 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

Bibliographic Details

Main Authors:	Sabariegos, R., Nadal, A., Beguiristain, N., Piron, M., Gómez, J.
Format:	journal article biblioteca
Language:	English
Published:	Wiley 2004
Subjects:	Hepatitis C virus, tRNA-like, Ribozymes, Ribonuclease P,
Online Access:	http://hdl.handle.net/20.500.12792/3959 http://hdl.handle.net/10261/294502
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