DNA methylation increases throughout Arabidopsis development
We used amplified fragment length polymorphisms (AFLP) to analyze the stability of DNA methylation throughout Arabidopsis development. AFLP can detect genome-wide changes in cytosine methylation produced by DNA demethylation agents, such as 5-azacytidine, or specific mutations at the DDM1 locus. In both cases, cytosine demethylation is associated with a general increase in the presence of amplified fragments. Using this approach, we followed DNA methylation at methylation sensitive restriction sites throughout Arabidopsis development. The results show a progressive DNA methylation trend from cotyledons to vegetative organs to reproductive organs. © Springer-Verlag 2005.
Saved in:
Main Authors: | Ruiz-García, L., Cervera, M. T., Martínez-Zapater, J. M. |
---|---|
Format: | journal article biblioteca |
Language: | English |
Published: |
Springer
2005
|
Subjects: | Arabidopsis, 5-azacytidine, ddm1 mutant, DNA methylation pattern, Plant development, |
Online Access: | http://hdl.handle.net/20.500.12792/3934 http://hdl.handle.net/10261/294493 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Analysis of DNA methylation in Arabidopsis thaliana based on methylation-sensitive AFLP markers
by: Cervera, M. T., et al.
Published: (2002) -
Quasispecies dynamics and RNA virus extinction
by: Domingo, E., et al.
Published: (2005) -
5-azacytidine: A promoter of epigenetic changes in the quest to improve plant somatic embryogenesis
by: PEDRO MANUEL OSORIO MONTALVO, et al. -
The DNA-methylation landscape of the root-knot nematode-induced pseudo-organ, the gall, in Arabidopsis, is dynamic, contrasting over time, and critically important for successful parasitism
by: Silva, Ana Cláudia, et al.
Published: (2022-07-24) -
A freezing-sensitive mutant of Arabidopsis, frs1, is a new aba3 allele
by: Llorente De Gracia, Francisco, et al.
Published: (2000)