DNA methylation increases throughout Arabidopsis development

We used amplified fragment length polymorphisms (AFLP) to analyze the stability of DNA methylation throughout Arabidopsis development. AFLP can detect genome-wide changes in cytosine methylation produced by DNA demethylation agents, such as 5-azacytidine, or specific mutations at the DDM1 locus. In both cases, cytosine demethylation is associated with a general increase in the presence of amplified fragments. Using this approach, we followed DNA methylation at methylation sensitive restriction sites throughout Arabidopsis development. The results show a progressive DNA methylation trend from cotyledons to vegetative organs to reproductive organs. © Springer-Verlag 2005.

Bibliographic Details

Main Authors:	Ruiz-García, L., Cervera, M. T., Martínez-Zapater, J. M.
Format:	journal article biblioteca
Language:	English
Published:	Springer 2005
Subjects:	Arabidopsis, 5-azacytidine, ddm1 mutant, DNA methylation pattern, Plant development,
Online Access:	http://hdl.handle.net/20.500.12792/3934 http://hdl.handle.net/10261/294493
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