Inoculation of newborn mice with non-coding regions of foot-and-mouth disease virus RNA can induce a rapid, solid and wide-range protection against viral infection
We have recently described the ability of in vitro-transcribed RNAs, mimicking structural domains in the 5' and 3' non-coding regions (NCRs) of the foot-and-mouth disease virus (FMDV) genome, to trigger the innate immune response in porcine cultured cells and mice. In this work, the antiviral effect exerted in vivo by these small synthetic non-infectious RNA molecules was analyzed extensively. The susceptibility of transfected newborn Swiss mice to FMDV challenge was tested using a wide range of viral doses. The level of protection depended on the specific RNA inoculated and was dose-dependent. The RNA giving the best protection was the internal ribosome entry site (IRES), followed by the transcripts corresponding to the S fragment. The time course of resistance to FMDV of the RNA-transfected mice was studied. Our results show the efficacy of these RNAs to prevent viral infection as well as to contain ongoing FMDV infection in certain time intervals. Protection proved to be independent of the serotype of FMDV used for challenge. These results support the potential use of the FMDV NCR transcripts as both prophylactic and therapeutic molecules for new FMDV control strategies. © 2011 Elsevier B.V.
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Format: | journal article biblioteca |
Language: | English |
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Elsevier
2011
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Subjects: | Foot-and-mouth disease, Viral non-coding regions, RNA antiviral activity, Anti-FMDV strategies, Innate immunity, |
Online Access: | http://hdl.handle.net/20.500.12792/3842 http://hdl.handle.net/10261/294454 |
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dig-inia-es-10261-2944542023-02-20T10:38:54Z Inoculation of newborn mice with non-coding regions of foot-and-mouth disease virus RNA can induce a rapid, solid and wide-range protection against viral infection Rodríguez-Pulido, M. Sobrino, F. Borrego, B. Sáiz, M. Foot-and-mouth disease Viral non-coding regions RNA antiviral activity Anti-FMDV strategies Innate immunity We have recently described the ability of in vitro-transcribed RNAs, mimicking structural domains in the 5' and 3' non-coding regions (NCRs) of the foot-and-mouth disease virus (FMDV) genome, to trigger the innate immune response in porcine cultured cells and mice. In this work, the antiviral effect exerted in vivo by these small synthetic non-infectious RNA molecules was analyzed extensively. The susceptibility of transfected newborn Swiss mice to FMDV challenge was tested using a wide range of viral doses. The level of protection depended on the specific RNA inoculated and was dose-dependent. The RNA giving the best protection was the internal ribosome entry site (IRES), followed by the transcripts corresponding to the S fragment. The time course of resistance to FMDV of the RNA-transfected mice was studied. Our results show the efficacy of these RNAs to prevent viral infection as well as to contain ongoing FMDV infection in certain time intervals. Protection proved to be independent of the serotype of FMDV used for challenge. These results support the potential use of the FMDV NCR transcripts as both prophylactic and therapeutic molecules for new FMDV control strategies. © 2011 Elsevier B.V. 2023-02-20T10:38:54Z 2023-02-20T10:38:54Z 2011 journal article Antiviral Research 92: 500-504 (2011) 0166-3542 http://hdl.handle.net/20.500.12792/3842 http://hdl.handle.net/10261/294454 10.1016/j.antiviral.2011.10.005 1872-9096 en none Elsevier |
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Foot-and-mouth disease Viral non-coding regions RNA antiviral activity Anti-FMDV strategies Innate immunity Foot-and-mouth disease Viral non-coding regions RNA antiviral activity Anti-FMDV strategies Innate immunity |
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Foot-and-mouth disease Viral non-coding regions RNA antiviral activity Anti-FMDV strategies Innate immunity Foot-and-mouth disease Viral non-coding regions RNA antiviral activity Anti-FMDV strategies Innate immunity Rodríguez-Pulido, M. Sobrino, F. Borrego, B. Sáiz, M. Inoculation of newborn mice with non-coding regions of foot-and-mouth disease virus RNA can induce a rapid, solid and wide-range protection against viral infection |
description |
We have recently described the ability of in vitro-transcribed RNAs, mimicking structural domains in the 5' and 3' non-coding regions (NCRs) of the foot-and-mouth disease virus (FMDV) genome, to trigger the innate immune response in porcine cultured cells and mice. In this work, the antiviral effect exerted in vivo by these small synthetic non-infectious RNA molecules was analyzed extensively. The susceptibility of transfected newborn Swiss mice to FMDV challenge was tested using a wide range of viral doses. The level of protection depended on the specific RNA inoculated and was dose-dependent. The RNA giving the best protection was the internal ribosome entry site (IRES), followed by the transcripts corresponding to the S fragment. The time course of resistance to FMDV of the RNA-transfected mice was studied. Our results show the efficacy of these RNAs to prevent viral infection as well as to contain ongoing FMDV infection in certain time intervals. Protection proved to be independent of the serotype of FMDV used for challenge. These results support the potential use of the FMDV NCR transcripts as both prophylactic and therapeutic molecules for new FMDV control strategies. © 2011 Elsevier B.V. |
format |
journal article |
topic_facet |
Foot-and-mouth disease Viral non-coding regions RNA antiviral activity Anti-FMDV strategies Innate immunity |
author |
Rodríguez-Pulido, M. Sobrino, F. Borrego, B. Sáiz, M. |
author_facet |
Rodríguez-Pulido, M. Sobrino, F. Borrego, B. Sáiz, M. |
author_sort |
Rodríguez-Pulido, M. |
title |
Inoculation of newborn mice with non-coding regions of foot-and-mouth disease virus RNA can induce a rapid, solid and wide-range protection against viral infection |
title_short |
Inoculation of newborn mice with non-coding regions of foot-and-mouth disease virus RNA can induce a rapid, solid and wide-range protection against viral infection |
title_full |
Inoculation of newborn mice with non-coding regions of foot-and-mouth disease virus RNA can induce a rapid, solid and wide-range protection against viral infection |
title_fullStr |
Inoculation of newborn mice with non-coding regions of foot-and-mouth disease virus RNA can induce a rapid, solid and wide-range protection against viral infection |
title_full_unstemmed |
Inoculation of newborn mice with non-coding regions of foot-and-mouth disease virus RNA can induce a rapid, solid and wide-range protection against viral infection |
title_sort |
inoculation of newborn mice with non-coding regions of foot-and-mouth disease virus rna can induce a rapid, solid and wide-range protection against viral infection |
publisher |
Elsevier |
publishDate |
2011 |
url |
http://hdl.handle.net/20.500.12792/3842 http://hdl.handle.net/10261/294454 |
work_keys_str_mv |
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