Equilocality and heterogeneity of constitutive heterochromatin in situ localization of two families of highly repetitive DNA in Dociostaurus genei (Orthoptera)
The chromosome complement of the grasshopper Dociostaurus genei is characterized by the presence of constitutive heterochromatin (C-bands) located in the centromeric regions of all the chromosomes and in the distal regions of some autosomes in the form of supernumerary segments. A sequence analysis was carried out to obtain information about the molecular characteristics of both heterochromatic regions. Two families of tandemly repetitive DNA (DgT2 and DgA3) from D. genei were cloned and characterized. Data obtained from in situ hybridization indicate that these families are located solely in the regions of constitutive heterochromatin. The DgT2 clone is representative of a family of sequences which mainly forms the centromeric C-bands in each chromosome of the complement. The DgA3 family is the major component of the distal C-bands (supernumerary segments) present in most of the autosomal pairs. These results show the existence in D. genei of two different families of repetitive DNA restricted to different chromosomal domains. We discuss these results in the light of the possible role of chromosomal disposition in the maintenance of the differences between heterochromatic DNA from different chromosomal regions and the homogenization of DNA sequences from equilocal chromosomal domains. © 1996 The Genetical Society of Great Britain.
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Language: | English |
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Springer Nature
1996
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Subjects: | Constitutive heterochromatin, Insect cytogenetics, Satellite DNA, |
Online Access: | http://hdl.handle.net/20.500.12792/3791 http://hdl.handle.net/10261/294443 |
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dig-inia-es-10261-2944432023-02-20T10:38:46Z Equilocality and heterogeneity of constitutive heterochromatin in situ localization of two families of highly repetitive DNA in Dociostaurus genei (Orthoptera) Rodríguez Iñigo, E. Fernández-Calvín, B. Capel, J. García De La Vega, C. Constitutive heterochromatin Insect cytogenetics Satellite DNA The chromosome complement of the grasshopper Dociostaurus genei is characterized by the presence of constitutive heterochromatin (C-bands) located in the centromeric regions of all the chromosomes and in the distal regions of some autosomes in the form of supernumerary segments. A sequence analysis was carried out to obtain information about the molecular characteristics of both heterochromatic regions. Two families of tandemly repetitive DNA (DgT2 and DgA3) from D. genei were cloned and characterized. Data obtained from in situ hybridization indicate that these families are located solely in the regions of constitutive heterochromatin. The DgT2 clone is representative of a family of sequences which mainly forms the centromeric C-bands in each chromosome of the complement. The DgA3 family is the major component of the distal C-bands (supernumerary segments) present in most of the autosomal pairs. These results show the existence in D. genei of two different families of repetitive DNA restricted to different chromosomal domains. We discuss these results in the light of the possible role of chromosomal disposition in the maintenance of the differences between heterochromatic DNA from different chromosomal regions and the homogenization of DNA sequences from equilocal chromosomal domains. © 1996 The Genetical Society of Great Britain. 2023-02-20T10:38:46Z 2023-02-20T10:38:46Z 1996 journal article HeredIty 76: 70-76 (1996) 0018-067X http://hdl.handle.net/20.500.12792/3791 http://hdl.handle.net/10261/294443 10.1038/hdy.1996.9 1365-2540 en none Springer Nature |
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Constitutive heterochromatin Insect cytogenetics Satellite DNA Constitutive heterochromatin Insect cytogenetics Satellite DNA |
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Constitutive heterochromatin Insect cytogenetics Satellite DNA Constitutive heterochromatin Insect cytogenetics Satellite DNA Rodríguez Iñigo, E. Fernández-Calvín, B. Capel, J. García De La Vega, C. Equilocality and heterogeneity of constitutive heterochromatin in situ localization of two families of highly repetitive DNA in Dociostaurus genei (Orthoptera) |
description |
The chromosome complement of the grasshopper Dociostaurus genei is characterized by the presence of constitutive heterochromatin (C-bands) located in the centromeric regions of all the chromosomes and in the distal regions of some autosomes in the form of supernumerary segments. A sequence analysis was carried out to obtain information about the molecular characteristics of both heterochromatic regions. Two families of tandemly repetitive DNA (DgT2 and DgA3) from D. genei were cloned and characterized. Data obtained from in situ hybridization indicate that these families are located solely in the regions of constitutive heterochromatin. The DgT2 clone is representative of a family of sequences which mainly forms the centromeric C-bands in each chromosome of the complement. The DgA3 family is the major component of the distal C-bands (supernumerary segments) present in most of the autosomal pairs. These results show the existence in D. genei of two different families of repetitive DNA restricted to different chromosomal domains. We discuss these results in the light of the possible role of chromosomal disposition in the maintenance of the differences between heterochromatic DNA from different chromosomal regions and the homogenization of DNA sequences from equilocal chromosomal domains. © 1996 The Genetical Society of Great Britain. |
format |
journal article |
topic_facet |
Constitutive heterochromatin Insect cytogenetics Satellite DNA |
author |
Rodríguez Iñigo, E. Fernández-Calvín, B. Capel, J. García De La Vega, C. |
author_facet |
Rodríguez Iñigo, E. Fernández-Calvín, B. Capel, J. García De La Vega, C. |
author_sort |
Rodríguez Iñigo, E. |
title |
Equilocality and heterogeneity of constitutive heterochromatin in situ localization of two families of highly repetitive DNA in Dociostaurus genei (Orthoptera) |
title_short |
Equilocality and heterogeneity of constitutive heterochromatin in situ localization of two families of highly repetitive DNA in Dociostaurus genei (Orthoptera) |
title_full |
Equilocality and heterogeneity of constitutive heterochromatin in situ localization of two families of highly repetitive DNA in Dociostaurus genei (Orthoptera) |
title_fullStr |
Equilocality and heterogeneity of constitutive heterochromatin in situ localization of two families of highly repetitive DNA in Dociostaurus genei (Orthoptera) |
title_full_unstemmed |
Equilocality and heterogeneity of constitutive heterochromatin in situ localization of two families of highly repetitive DNA in Dociostaurus genei (Orthoptera) |
title_sort |
equilocality and heterogeneity of constitutive heterochromatin in situ localization of two families of highly repetitive dna in dociostaurus genei (orthoptera) |
publisher |
Springer Nature |
publishDate |
1996 |
url |
http://hdl.handle.net/20.500.12792/3791 http://hdl.handle.net/10261/294443 |
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