Activity of 5-enolpyruvylshikimate-3-phosphate synthase in glyphosate treated and untreated barley

Activity of 5-enolpyruvylshikimate-3-phosphate synthase in glyphosate treated and untreated barley

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EPSPS (5-enolpyruvilshikimate-3-phosphate syn-thase) inhibition has been described as the main target for the herbicide glyphosate. Total soluble protein concentration and EPSPS activity were measured after 4 hr, 1 day and 7 day of glyphosate treatment, at a dose equivalent to 0.94 Kg ha-1. After seven days of glyphosate treatment, EPSPS activity reduced to 80% as compared to the activity in untreated seedlings. Glyphosate at 2 μM concentration inhibited EPSPS by 18% at a PEP concentration of 1 mM, but inhibition increased to 64% when PEP concentration was 0.1 mM. Moreover, 1.5 μM glyphosate concentration reduced EPSPS activity by 50% at 0.1 mM of PEP, instead 0.1 mM of glyphosate was necessary when 1 mM was present in the reactions.

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Bibliographic Details
Main Authors: Rebordinos, L., Escorial Bonet, María Concepción, Garcia-Baudin, J. M., Chueca, M. C.
Format: journal article biblioteca
Language:English
Published: Centers for Disease Control and Prevention (U.S.) 1996
Online Access:http://hdl.handle.net/20.500.12792/3679
http://hdl.handle.net/10261/294412
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spelling dig-inia-es-10261-2944122023-02-20T10:38:29Z Activity of 5-enolpyruvylshikimate-3-phosphate synthase in glyphosate treated and untreated barley Rebordinos, L. Escorial Bonet, María Concepción Garcia-Baudin, J. M. Chueca, M. C. EPSPS (5-enolpyruvilshikimate-3-phosphate syn-thase) inhibition has been described as the main target for the herbicide glyphosate. Total soluble protein concentration and EPSPS activity were measured after 4 hr, 1 day and 7 day of glyphosate treatment, at a dose equivalent to 0.94 Kg ha-1. After seven days of glyphosate treatment, EPSPS activity reduced to 80% as compared to the activity in untreated seedlings. Glyphosate at 2 μM concentration inhibited EPSPS by 18% at a PEP concentration of 1 mM, but inhibition increased to 64% when PEP concentration was 0.1 mM. Moreover, 1.5 μM glyphosate concentration reduced EPSPS activity by 50% at 0.1 mM of PEP, instead 0.1 mM of glyphosate was necessary when 1 mM was present in the reactions. 2023-02-20T10:38:29Z 2023-02-20T10:38:29Z 1996 journal article Indian Journal of Experimental Biology 34(11): 1172-1174 (1996) http://hdl.handle.net/20.500.12792/3679 http://hdl.handle.net/10261/294412 1080-6040 en none Centers for Disease Control and Prevention (U.S.)
institution INIA ES
collection DSpace
country España
countrycode ES
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access En linea
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tag biblioteca
region Europa del Sur
libraryname Biblioteca del INIA España
language English
description EPSPS (5-enolpyruvilshikimate-3-phosphate syn-thase) inhibition has been described as the main target for the herbicide glyphosate. Total soluble protein concentration and EPSPS activity were measured after 4 hr, 1 day and 7 day of glyphosate treatment, at a dose equivalent to 0.94 Kg ha-1. After seven days of glyphosate treatment, EPSPS activity reduced to 80% as compared to the activity in untreated seedlings. Glyphosate at 2 μM concentration inhibited EPSPS by 18% at a PEP concentration of 1 mM, but inhibition increased to 64% when PEP concentration was 0.1 mM. Moreover, 1.5 μM glyphosate concentration reduced EPSPS activity by 50% at 0.1 mM of PEP, instead 0.1 mM of glyphosate was necessary when 1 mM was present in the reactions.
format journal article
author Rebordinos, L.
Escorial Bonet, María Concepción
Garcia-Baudin, J. M.
Chueca, M. C.
spellingShingle Rebordinos, L.
Escorial Bonet, María Concepción
Garcia-Baudin, J. M.
Chueca, M. C.
Activity of 5-enolpyruvylshikimate-3-phosphate synthase in glyphosate treated and untreated barley
author_facet Rebordinos, L.
Escorial Bonet, María Concepción
Garcia-Baudin, J. M.
Chueca, M. C.
author_sort Rebordinos, L.
title Activity of 5-enolpyruvylshikimate-3-phosphate synthase in glyphosate treated and untreated barley
title_short Activity of 5-enolpyruvylshikimate-3-phosphate synthase in glyphosate treated and untreated barley
title_full Activity of 5-enolpyruvylshikimate-3-phosphate synthase in glyphosate treated and untreated barley
title_fullStr Activity of 5-enolpyruvylshikimate-3-phosphate synthase in glyphosate treated and untreated barley
title_full_unstemmed Activity of 5-enolpyruvylshikimate-3-phosphate synthase in glyphosate treated and untreated barley
title_sort activity of 5-enolpyruvylshikimate-3-phosphate synthase in glyphosate treated and untreated barley
publisher Centers for Disease Control and Prevention (U.S.)
publishDate 1996
url http://hdl.handle.net/20.500.12792/3679
http://hdl.handle.net/10261/294412
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