RNA immunization can protect mice against foot-and-mouth disease virus
In previous work we have reported the immunization of swine using in vitro-transcribed foot-and-mouth disease virus (FMDV) RNA. With the aim of testing whether RNA-induced immunization can mediate protection against viral infection, a group of Swiss adult mice was inoculated with FMDV infectious transcripts. In most inoculated animals viral RNA was detected in serum at 48-72 h postinoculation. A group of the RNA-inoculated mice (11 out of 19) developed significant titers of neutralizing antibodies against FMDV. Among those animals that were successfully challenged with infectious virus (15 out of 19), three out of the eight animals immunized upon RNA inoculation were protected, as infectious virus could not be isolated from sera but specific anti-FMDV antibodies could be readily detected. These results suggest the potential of the inoculation of genetically engineered FMDV RNA for virulence and protection assays in the murine model and allow to explore the suitability of RNA-based FMDV vaccination in natural host animals. © 2009 Elsevier B.V. All rights reserved.
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| Format: | journal article biblioteca |
| Language: | English |
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Elsevier
2010
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| Subjects: | Foot-and-mouth disease virus, RNA vaccines, Genetic immunization, In vivo, Foot-and-mouth disease vaccine, |
| Online Access: | http://hdl.handle.net/20.500.12792/3601 http://hdl.handle.net/10261/294397 |
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dig-inia-es-10261-2943972023-02-20T10:38:19Z RNA immunization can protect mice against foot-and-mouth disease virus Pulido, M. R. Sobrino, F. Borrego, B. Sáiz, M. Foot-and-mouth disease virus RNA vaccines Genetic immunization In vivo Foot-and-mouth disease vaccine In previous work we have reported the immunization of swine using in vitro-transcribed foot-and-mouth disease virus (FMDV) RNA. With the aim of testing whether RNA-induced immunization can mediate protection against viral infection, a group of Swiss adult mice was inoculated with FMDV infectious transcripts. In most inoculated animals viral RNA was detected in serum at 48-72 h postinoculation. A group of the RNA-inoculated mice (11 out of 19) developed significant titers of neutralizing antibodies against FMDV. Among those animals that were successfully challenged with infectious virus (15 out of 19), three out of the eight animals immunized upon RNA inoculation were protected, as infectious virus could not be isolated from sera but specific anti-FMDV antibodies could be readily detected. These results suggest the potential of the inoculation of genetically engineered FMDV RNA for virulence and protection assays in the murine model and allow to explore the suitability of RNA-based FMDV vaccination in natural host animals. © 2009 Elsevier B.V. All rights reserved. 2023-02-20T10:38:19Z 2023-02-20T10:38:19Z 2010 journal article Antiviral Research 85(3): 556-558 (2010) 0166-3542 http://hdl.handle.net/20.500.12792/3601 http://hdl.handle.net/10261/294397 10.1016/j.antiviral.2009.12.005 1872-9096 en none Elsevier |
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Foot-and-mouth disease virus RNA vaccines Genetic immunization In vivo Foot-and-mouth disease vaccine Foot-and-mouth disease virus RNA vaccines Genetic immunization In vivo Foot-and-mouth disease vaccine |
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Foot-and-mouth disease virus RNA vaccines Genetic immunization In vivo Foot-and-mouth disease vaccine Foot-and-mouth disease virus RNA vaccines Genetic immunization In vivo Foot-and-mouth disease vaccine Pulido, M. R. Sobrino, F. Borrego, B. Sáiz, M. RNA immunization can protect mice against foot-and-mouth disease virus |
| description |
In previous work we have reported the immunization of swine using in vitro-transcribed foot-and-mouth disease virus (FMDV) RNA. With the aim of testing whether RNA-induced immunization can mediate protection against viral infection, a group of Swiss adult mice was inoculated with FMDV infectious transcripts. In most inoculated animals viral RNA was detected in serum at 48-72 h postinoculation. A group of the RNA-inoculated mice (11 out of 19) developed significant titers of neutralizing antibodies against FMDV. Among those animals that were successfully challenged with infectious virus (15 out of 19), three out of the eight animals immunized upon RNA inoculation were protected, as infectious virus could not be isolated from sera but specific anti-FMDV antibodies could be readily detected. These results suggest the potential of the inoculation of genetically engineered FMDV RNA for virulence and protection assays in the murine model and allow to explore the suitability of RNA-based FMDV vaccination in natural host animals. © 2009 Elsevier B.V. All rights reserved. |
| format |
journal article |
| topic_facet |
Foot-and-mouth disease virus RNA vaccines Genetic immunization In vivo Foot-and-mouth disease vaccine |
| author |
Pulido, M. R. Sobrino, F. Borrego, B. Sáiz, M. |
| author_facet |
Pulido, M. R. Sobrino, F. Borrego, B. Sáiz, M. |
| author_sort |
Pulido, M. R. |
| title |
RNA immunization can protect mice against foot-and-mouth disease virus |
| title_short |
RNA immunization can protect mice against foot-and-mouth disease virus |
| title_full |
RNA immunization can protect mice against foot-and-mouth disease virus |
| title_fullStr |
RNA immunization can protect mice against foot-and-mouth disease virus |
| title_full_unstemmed |
RNA immunization can protect mice against foot-and-mouth disease virus |
| title_sort |
rna immunization can protect mice against foot-and-mouth disease virus |
| publisher |
Elsevier |
| publishDate |
2010 |
| url |
http://hdl.handle.net/20.500.12792/3601 http://hdl.handle.net/10261/294397 |
| work_keys_str_mv |
AT pulidomr rnaimmunizationcanprotectmiceagainstfootandmouthdiseasevirus AT sobrinof rnaimmunizationcanprotectmiceagainstfootandmouthdiseasevirus AT borregob rnaimmunizationcanprotectmiceagainstfootandmouthdiseasevirus AT saizm rnaimmunizationcanprotectmiceagainstfootandmouthdiseasevirus |
| _version_ |
1767603608832966656 |