Development of an enhanced bovine viral diarrhea virus subunitvaccine based on E2 glycoprotein fused to a single chain antibody which targets to antigen-presenting cells
Bovine viral diarrhea virus (BVDV) is an important cause of economic losses world-wide. E2 is an immunodominant protein and a promising candidate to develop subunit vaccines.To improve its immunogenicity, a truncated E2 (tE2) was fused to a single chain antibody named APCH, which targets to antigen-presenting cells. APCH-tE2 and tE2 proteins were expressed inthe baculovirus system and their immunogenicity was firstly compared in guinea pigs. APCH-tE2 vaccine was the best one to evoke a humoral response, and for this reason, it was selected fora cattle vaccination experiment. All the bovines immunized with 1.5 µg of APCH-tE2 developedhigh levels of neutralizing antibodies against BVDV up to a year post-immunization, demon-strating its significant potential as a subunit vaccine. This novel vaccine is undergoing scale-upand was transferred to the private sector. Nowadays, it is being evaluated for registration asthe first Argentinean subunit vaccine for cattle. © 2014 Asociación Argentina de Microbiología.
Main Authors: | , , , , , , |
---|---|
Format: | journal article biblioteca |
Language: | English |
Published: |
Elsevier
2015
|
Subjects: | E2 glycoprotein, Single chain antibody, Subunit vaccine, Baculovirus, Bovine viral diarrhea virus, |
Online Access: | http://hdl.handle.net/20.500.12792/3357 http://hdl.handle.net/10261/294308 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-inia-es-10261-294308 |
---|---|
record_format |
koha |
spelling |
dig-inia-es-10261-2943082023-02-20T10:37:27Z Development of an enhanced bovine viral diarrhea virus subunitvaccine based on E2 glycoprotein fused to a single chain antibody which targets to antigen-presenting cells Pecora, A. Malacari, D. A. Pérez Aguirreburualde, M. S. Bellido, D. Escribano, J. M. Dus Santos, M. J. Wigdorovitz, A. E2 glycoprotein Single chain antibody Subunit vaccine Baculovirus Bovine viral diarrhea virus Bovine viral diarrhea virus (BVDV) is an important cause of economic losses world-wide. E2 is an immunodominant protein and a promising candidate to develop subunit vaccines.To improve its immunogenicity, a truncated E2 (tE2) was fused to a single chain antibody named APCH, which targets to antigen-presenting cells. APCH-tE2 and tE2 proteins were expressed inthe baculovirus system and their immunogenicity was firstly compared in guinea pigs. APCH-tE2 vaccine was the best one to evoke a humoral response, and for this reason, it was selected fora cattle vaccination experiment. All the bovines immunized with 1.5 µg of APCH-tE2 developedhigh levels of neutralizing antibodies against BVDV up to a year post-immunization, demon-strating its significant potential as a subunit vaccine. This novel vaccine is undergoing scale-upand was transferred to the private sector. Nowadays, it is being evaluated for registration asthe first Argentinean subunit vaccine for cattle. © 2014 Asociación Argentina de Microbiología. 2023-02-20T10:37:27Z 2023-02-20T10:37:27Z 2015 journal article Revista Argentina de Microbiologia 47(1): 4-8 (2015 0325-7541 http://hdl.handle.net/20.500.12792/3357 http://hdl.handle.net/10261/294308 10.1016/j.ram.2014.12.004 1851-7617 en open Elsevier |
institution |
INIA ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-inia-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del INIA España |
language |
English |
topic |
E2 glycoprotein Single chain antibody Subunit vaccine Baculovirus Bovine viral diarrhea virus E2 glycoprotein Single chain antibody Subunit vaccine Baculovirus Bovine viral diarrhea virus |
spellingShingle |
E2 glycoprotein Single chain antibody Subunit vaccine Baculovirus Bovine viral diarrhea virus E2 glycoprotein Single chain antibody Subunit vaccine Baculovirus Bovine viral diarrhea virus Pecora, A. Malacari, D. A. Pérez Aguirreburualde, M. S. Bellido, D. Escribano, J. M. Dus Santos, M. J. Wigdorovitz, A. Development of an enhanced bovine viral diarrhea virus subunitvaccine based on E2 glycoprotein fused to a single chain antibody which targets to antigen-presenting cells |
description |
Bovine viral diarrhea virus (BVDV) is an important cause of economic losses world-wide. E2 is an immunodominant protein and a promising candidate to develop subunit vaccines.To improve its immunogenicity, a truncated E2 (tE2) was fused to a single chain antibody named APCH, which targets to antigen-presenting cells. APCH-tE2 and tE2 proteins were expressed inthe baculovirus system and their immunogenicity was firstly compared in guinea pigs. APCH-tE2 vaccine was the best one to evoke a humoral response, and for this reason, it was selected fora cattle vaccination experiment. All the bovines immunized with 1.5 µg of APCH-tE2 developedhigh levels of neutralizing antibodies against BVDV up to a year post-immunization, demon-strating its significant potential as a subunit vaccine. This novel vaccine is undergoing scale-upand was transferred to the private sector. Nowadays, it is being evaluated for registration asthe first Argentinean subunit vaccine for cattle. © 2014 Asociación Argentina de Microbiología. |
format |
journal article |
topic_facet |
E2 glycoprotein Single chain antibody Subunit vaccine Baculovirus Bovine viral diarrhea virus |
author |
Pecora, A. Malacari, D. A. Pérez Aguirreburualde, M. S. Bellido, D. Escribano, J. M. Dus Santos, M. J. Wigdorovitz, A. |
author_facet |
Pecora, A. Malacari, D. A. Pérez Aguirreburualde, M. S. Bellido, D. Escribano, J. M. Dus Santos, M. J. Wigdorovitz, A. |
author_sort |
Pecora, A. |
title |
Development of an enhanced bovine viral diarrhea virus subunitvaccine based on E2 glycoprotein fused to a single chain antibody which targets to antigen-presenting cells |
title_short |
Development of an enhanced bovine viral diarrhea virus subunitvaccine based on E2 glycoprotein fused to a single chain antibody which targets to antigen-presenting cells |
title_full |
Development of an enhanced bovine viral diarrhea virus subunitvaccine based on E2 glycoprotein fused to a single chain antibody which targets to antigen-presenting cells |
title_fullStr |
Development of an enhanced bovine viral diarrhea virus subunitvaccine based on E2 glycoprotein fused to a single chain antibody which targets to antigen-presenting cells |
title_full_unstemmed |
Development of an enhanced bovine viral diarrhea virus subunitvaccine based on E2 glycoprotein fused to a single chain antibody which targets to antigen-presenting cells |
title_sort |
development of an enhanced bovine viral diarrhea virus subunitvaccine based on e2 glycoprotein fused to a single chain antibody which targets to antigen-presenting cells |
publisher |
Elsevier |
publishDate |
2015 |
url |
http://hdl.handle.net/20.500.12792/3357 http://hdl.handle.net/10261/294308 |
work_keys_str_mv |
AT pecoraa developmentofanenhancedbovineviraldiarrheavirussubunitvaccinebasedone2glycoproteinfusedtoasinglechainantibodywhichtargetstoantigenpresentingcells AT malacarida developmentofanenhancedbovineviraldiarrheavirussubunitvaccinebasedone2glycoproteinfusedtoasinglechainantibodywhichtargetstoantigenpresentingcells AT perezaguirreburualdems developmentofanenhancedbovineviraldiarrheavirussubunitvaccinebasedone2glycoproteinfusedtoasinglechainantibodywhichtargetstoantigenpresentingcells AT bellidod developmentofanenhancedbovineviraldiarrheavirussubunitvaccinebasedone2glycoproteinfusedtoasinglechainantibodywhichtargetstoantigenpresentingcells AT escribanojm developmentofanenhancedbovineviraldiarrheavirussubunitvaccinebasedone2glycoproteinfusedtoasinglechainantibodywhichtargetstoantigenpresentingcells AT dussantosmj developmentofanenhancedbovineviraldiarrheavirussubunitvaccinebasedone2glycoproteinfusedtoasinglechainantibodywhichtargetstoantigenpresentingcells AT wigdorovitza developmentofanenhancedbovineviraldiarrheavirussubunitvaccinebasedone2glycoproteinfusedtoasinglechainantibodywhichtargetstoantigenpresentingcells |
_version_ |
1767603596601327616 |