Lens culinaris, Phaseolus vulgaris and Vicia faba lectins specifically trigger IL-8 production by the human colon carcinoma cell line Caco-2
Cultured Caco-2 cells were stimulated with Lens culinaris, Phaseolus vulgaris and Vicia faba lectins. The production of IL-1, IL-6, IL-8 and MCP-1 was measured by ELISA and RT-PCR. IL-8 production appeared to be specifically triggered upon stimulation with all three lectins used, since none of the other cytokines tested were produced. The IL-8 secreted may induce the extravasation of activated neutrophils and generate tissue damage. A similar mechanism may be implicated in the lesions observed after infection by some enteric pathogens, with lectin-like domains on their membrane. Finally, this model may be suitable one to study the regulation of IL-8 production. (C) 2000 Academic Press.
Main Authors: | , , , , , , |
---|---|
Format: | journal article biblioteca |
Language: | English |
Published: |
Elsevier
2000
|
Subjects: | Caco-2, IL-8, Lectins, |
Online Access: | http://hdl.handle.net/20.500.12792/3836 http://hdl.handle.net/10261/294164 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-inia-es-10261-294164 |
---|---|
record_format |
koha |
spelling |
dig-inia-es-10261-2941642023-02-20T10:35:58Z Lens culinaris, Phaseolus vulgaris and Vicia faba lectins specifically trigger IL-8 production by the human colon carcinoma cell line Caco-2 Rodríguez-Juan, C. Pérez-Blas, M. Suárez-García, E. López-Suárez, J. C. Múzquiz, M. Cuadrado Hoyos, María Carmen Martín-Villa, J. M. Caco-2 IL-8 Lectins Cultured Caco-2 cells were stimulated with Lens culinaris, Phaseolus vulgaris and Vicia faba lectins. The production of IL-1, IL-6, IL-8 and MCP-1 was measured by ELISA and RT-PCR. IL-8 production appeared to be specifically triggered upon stimulation with all three lectins used, since none of the other cytokines tested were produced. The IL-8 secreted may induce the extravasation of activated neutrophils and generate tissue damage. A similar mechanism may be implicated in the lesions observed after infection by some enteric pathogens, with lectin-like domains on their membrane. Finally, this model may be suitable one to study the regulation of IL-8 production. (C) 2000 Academic Press. 2023-02-20T10:35:58Z 2023-02-20T10:35:58Z 2000 journal article Cytokine 12(8): 1284-1287 (2000) 1043-4666 http://hdl.handle.net/20.500.12792/3836 http://hdl.handle.net/10261/294164 10.1006/cyto.1999.0731 1096-0023 en none Elsevier |
institution |
INIA ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-inia-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del INIA España |
language |
English |
topic |
Caco-2 IL-8 Lectins Caco-2 IL-8 Lectins |
spellingShingle |
Caco-2 IL-8 Lectins Caco-2 IL-8 Lectins Rodríguez-Juan, C. Pérez-Blas, M. Suárez-García, E. López-Suárez, J. C. Múzquiz, M. Cuadrado Hoyos, María Carmen Martín-Villa, J. M. Lens culinaris, Phaseolus vulgaris and Vicia faba lectins specifically trigger IL-8 production by the human colon carcinoma cell line Caco-2 |
description |
Cultured Caco-2 cells were stimulated with Lens culinaris, Phaseolus vulgaris and Vicia faba lectins. The production of IL-1, IL-6, IL-8 and MCP-1 was measured by ELISA and RT-PCR. IL-8 production appeared to be specifically triggered upon stimulation with all three lectins used, since none of the other cytokines tested were produced. The IL-8 secreted may induce the extravasation of activated neutrophils and generate tissue damage. A similar mechanism may be implicated in the lesions observed after infection by some enteric pathogens, with lectin-like domains on their membrane. Finally, this model may be suitable one to study the regulation of IL-8 production. (C) 2000 Academic Press. |
format |
journal article |
topic_facet |
Caco-2 IL-8 Lectins |
author |
Rodríguez-Juan, C. Pérez-Blas, M. Suárez-García, E. López-Suárez, J. C. Múzquiz, M. Cuadrado Hoyos, María Carmen Martín-Villa, J. M. |
author_facet |
Rodríguez-Juan, C. Pérez-Blas, M. Suárez-García, E. López-Suárez, J. C. Múzquiz, M. Cuadrado Hoyos, María Carmen Martín-Villa, J. M. |
author_sort |
Rodríguez-Juan, C. |
title |
Lens culinaris, Phaseolus vulgaris and Vicia faba lectins specifically trigger IL-8 production by the human colon carcinoma cell line Caco-2 |
title_short |
Lens culinaris, Phaseolus vulgaris and Vicia faba lectins specifically trigger IL-8 production by the human colon carcinoma cell line Caco-2 |
title_full |
Lens culinaris, Phaseolus vulgaris and Vicia faba lectins specifically trigger IL-8 production by the human colon carcinoma cell line Caco-2 |
title_fullStr |
Lens culinaris, Phaseolus vulgaris and Vicia faba lectins specifically trigger IL-8 production by the human colon carcinoma cell line Caco-2 |
title_full_unstemmed |
Lens culinaris, Phaseolus vulgaris and Vicia faba lectins specifically trigger IL-8 production by the human colon carcinoma cell line Caco-2 |
title_sort |
lens culinaris, phaseolus vulgaris and vicia faba lectins specifically trigger il-8 production by the human colon carcinoma cell line caco-2 |
publisher |
Elsevier |
publishDate |
2000 |
url |
http://hdl.handle.net/20.500.12792/3836 http://hdl.handle.net/10261/294164 |
work_keys_str_mv |
AT rodriguezjuanc lensculinarisphaseolusvulgarisandviciafabalectinsspecificallytriggeril8productionbythehumancoloncarcinomacelllinecaco2 AT perezblasm lensculinarisphaseolusvulgarisandviciafabalectinsspecificallytriggeril8productionbythehumancoloncarcinomacelllinecaco2 AT suarezgarciae lensculinarisphaseolusvulgarisandviciafabalectinsspecificallytriggeril8productionbythehumancoloncarcinomacelllinecaco2 AT lopezsuarezjc lensculinarisphaseolusvulgarisandviciafabalectinsspecificallytriggeril8productionbythehumancoloncarcinomacelllinecaco2 AT muzquizm lensculinarisphaseolusvulgarisandviciafabalectinsspecificallytriggeril8productionbythehumancoloncarcinomacelllinecaco2 AT cuadradohoyosmariacarmen lensculinarisphaseolusvulgarisandviciafabalectinsspecificallytriggeril8productionbythehumancoloncarcinomacelllinecaco2 AT martinvillajm lensculinarisphaseolusvulgarisandviciafabalectinsspecificallytriggeril8productionbythehumancoloncarcinomacelllinecaco2 |
_version_ |
1767603576397365248 |