Sequence and analysis of a swinepox virus homologue of the vaccinia virus major envelope protein P37 (F13L)
P37 (F13L gene product), the most abundant protein in the envelope of the extracellular virus form of the prototype poxvirus, vaccinia virus (VV), is a crucial player in the process leading to acquisition of the envelope, virus egress and transmission. We have cloned and sequenced a swinepox virus (SPV) gene homologous to VV F13L. The SPV gene product, termed P42, was 54% identical to P37, the VV F13L gene product, and, among the poxviruses, was most similar (73% identity) to the myxoma virus homologue. The SPV P42 gene contained late transcription signals and was expressed only at late times during infection. The protein was palmitylated, and showed an intracellular distribution similar to that of VV P37, both by immunofluorescence and by subcellular fractionation. As with VV P37, SPV P42 was incorporated in extracellular enveloped SPV particles, but was absent from the intracellular mature virus form. To check the ability of SPV P42 to function in the context of VV infection, we inserted the SPV gene into a VV deficient in P37, which is severely blocked in virus envelopment and cell-to-cell transmission. Despite correct expression of SPV P42, the resulting recombinant VV showed no rescue of extracellular virus formation or cell-to-cell virus spread. The lack of function of SPV P42 in the VV genetic background suggests that specific interactions between SPV P42 or VV P37 and other viral proteins is required to drive the envelopment process.
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Microbiology Society
2000
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Online Access: | http://hdl.handle.net/20.500.12792/2885 http://hdl.handle.net/10261/294133 |
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dig-inia-es-10261-2941332023-02-20T10:35:36Z Sequence and analysis of a swinepox virus homologue of the vaccinia virus major envelope protein P37 (F13L) Barcena Del Riego, Juan Ángelo Lorenzo Gilsanz, María Mar Sánchez-Puig Eyre, Juana María Blasco Lozano, Rafael P37 (F13L gene product), the most abundant protein in the envelope of the extracellular virus form of the prototype poxvirus, vaccinia virus (VV), is a crucial player in the process leading to acquisition of the envelope, virus egress and transmission. We have cloned and sequenced a swinepox virus (SPV) gene homologous to VV F13L. The SPV gene product, termed P42, was 54% identical to P37, the VV F13L gene product, and, among the poxviruses, was most similar (73% identity) to the myxoma virus homologue. The SPV P42 gene contained late transcription signals and was expressed only at late times during infection. The protein was palmitylated, and showed an intracellular distribution similar to that of VV P37, both by immunofluorescence and by subcellular fractionation. As with VV P37, SPV P42 was incorporated in extracellular enveloped SPV particles, but was absent from the intracellular mature virus form. To check the ability of SPV P42 to function in the context of VV infection, we inserted the SPV gene into a VV deficient in P37, which is severely blocked in virus envelopment and cell-to-cell transmission. Despite correct expression of SPV P42, the resulting recombinant VV showed no rescue of extracellular virus formation or cell-to-cell virus spread. The lack of function of SPV P42 in the VV genetic background suggests that specific interactions between SPV P42 or VV P37 and other viral proteins is required to drive the envelopment process. 2023-02-20T10:35:36Z 2023-02-20T10:35:36Z 2000 journal article Journal of General Virology 81(4): 1073-1085 (2000) 0022-1317 http://hdl.handle.net/20.500.12792/2885 http://hdl.handle.net/10261/294133 10.1099/0022-1317-81-4-1073 1465-2099 en none Microbiology Society |
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P37 (F13L gene product), the most abundant protein in the envelope of the extracellular virus form of the prototype poxvirus, vaccinia virus (VV), is a crucial player in the process leading to acquisition of the envelope, virus egress and transmission. We have cloned and sequenced a swinepox virus (SPV) gene homologous to VV F13L. The SPV gene product, termed P42, was 54% identical to P37, the VV F13L gene product, and, among the poxviruses, was most similar (73% identity) to the myxoma virus homologue. The SPV P42 gene contained late transcription signals and was expressed only at late times during infection. The protein was palmitylated, and showed an intracellular distribution similar to that of VV P37, both by immunofluorescence and by subcellular fractionation. As with VV P37, SPV P42 was incorporated in extracellular enveloped SPV particles, but was absent from the intracellular mature virus form. To check the ability of SPV P42 to function in the context of VV infection, we inserted the SPV gene into a VV deficient in P37, which is severely blocked in virus envelopment and cell-to-cell transmission. Despite correct expression of SPV P42, the resulting recombinant VV showed no rescue of extracellular virus formation or cell-to-cell virus spread. The lack of function of SPV P42 in the VV genetic background suggests that specific interactions between SPV P42 or VV P37 and other viral proteins is required to drive the envelopment process. |
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journal article |
author |
Barcena Del Riego, Juan Ángelo Lorenzo Gilsanz, María Mar Sánchez-Puig Eyre, Juana María Blasco Lozano, Rafael |
spellingShingle |
Barcena Del Riego, Juan Ángelo Lorenzo Gilsanz, María Mar Sánchez-Puig Eyre, Juana María Blasco Lozano, Rafael Sequence and analysis of a swinepox virus homologue of the vaccinia virus major envelope protein P37 (F13L) |
author_facet |
Barcena Del Riego, Juan Ángelo Lorenzo Gilsanz, María Mar Sánchez-Puig Eyre, Juana María Blasco Lozano, Rafael |
author_sort |
Barcena Del Riego, Juan Ángelo |
title |
Sequence and analysis of a swinepox virus homologue of the vaccinia virus major envelope protein P37 (F13L) |
title_short |
Sequence and analysis of a swinepox virus homologue of the vaccinia virus major envelope protein P37 (F13L) |
title_full |
Sequence and analysis of a swinepox virus homologue of the vaccinia virus major envelope protein P37 (F13L) |
title_fullStr |
Sequence and analysis of a swinepox virus homologue of the vaccinia virus major envelope protein P37 (F13L) |
title_full_unstemmed |
Sequence and analysis of a swinepox virus homologue of the vaccinia virus major envelope protein P37 (F13L) |
title_sort |
sequence and analysis of a swinepox virus homologue of the vaccinia virus major envelope protein p37 (f13l) |
publisher |
Microbiology Society |
publishDate |
2000 |
url |
http://hdl.handle.net/20.500.12792/2885 http://hdl.handle.net/10261/294133 |
work_keys_str_mv |
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