The HVE/CAND1 gene is required for the early patterning of leaf venation in Arabidopsis
The hemivenata-1 (hve-1) recessive allele was isolated in a search for natural variations in the leaf venation pattern of Arabidopsis thaliana, where it was seen to cause extremely simple venation in vegetative leaves and cotyledons, increased shoot branching, and reduced root waving and fertility, traits that are reminiscent of some mutants deficient in auxin signaling. Reduced sensitivity to exogenous auxin was found in the hve-1 mutant, which otherwise displayed a wild-type response to auxin transport inhibitors. The HVE gene was positionally cloned and found to encode a CAND1 protein. The hve-1 mutation caused mis-splicing of the transcripts of the HVEICAND1 gene and a vein phenotype indistinguishable from that of hve-2 and hve-3, two putatively null T-DNA alleles. Inflorescence size and fertility were more affected by hve-2 and hve-3, suggesting that hve-1 is hypomorphic. The simple venation pattern of hve plants seems to arise from an early patterning defect. We found that HVE/CAND1 binds to CULLIN1, and that the venation patterns of axr1 and hve mutants are similar, which suggest that ubiquitin-mediated auxin signaling is required for venation patterning in laminar organs, the only exception being cauline leaves. Our analyses of double mutant and transgenic plants indicated that auxin transport and perception act independently to pattern leaf veins, and that the HVE/ CAND1 genes acts upstream of ATHB-8 at least in higher order veins, in a pathway that involves AXR1, but not LOP1, PIN1, CVP1 or CVP2.
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Language: | English |
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Company of Biologists
2006
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Subjects: | Arabidopsis, TIP120, CAND1, Venation pattern formation, Natural variation, |
Online Access: | http://hdl.handle.net/20.500.12792/4845 http://hdl.handle.net/10261/293989 |
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dig-inia-es-10261-2939892023-02-20T10:34:12Z The HVE/CAND1 gene is required for the early patterning of leaf venation in Arabidopsis Alonso-Peral, M. M. Candela, H. del Pozo, J. C. Martínez-Laborda, A. Ponce, M. R. Micol, J. L. Arabidopsis TIP120 CAND1 Venation pattern formation Natural variation The hemivenata-1 (hve-1) recessive allele was isolated in a search for natural variations in the leaf venation pattern of Arabidopsis thaliana, where it was seen to cause extremely simple venation in vegetative leaves and cotyledons, increased shoot branching, and reduced root waving and fertility, traits that are reminiscent of some mutants deficient in auxin signaling. Reduced sensitivity to exogenous auxin was found in the hve-1 mutant, which otherwise displayed a wild-type response to auxin transport inhibitors. The HVE gene was positionally cloned and found to encode a CAND1 protein. The hve-1 mutation caused mis-splicing of the transcripts of the HVEICAND1 gene and a vein phenotype indistinguishable from that of hve-2 and hve-3, two putatively null T-DNA alleles. Inflorescence size and fertility were more affected by hve-2 and hve-3, suggesting that hve-1 is hypomorphic. The simple venation pattern of hve plants seems to arise from an early patterning defect. We found that HVE/CAND1 binds to CULLIN1, and that the venation patterns of axr1 and hve mutants are similar, which suggest that ubiquitin-mediated auxin signaling is required for venation patterning in laminar organs, the only exception being cauline leaves. Our analyses of double mutant and transgenic plants indicated that auxin transport and perception act independently to pattern leaf veins, and that the HVE/ CAND1 genes acts upstream of ATHB-8 at least in higher order veins, in a pathway that involves AXR1, but not LOP1, PIN1, CVP1 or CVP2. 2023-02-20T10:34:12Z 2023-02-20T10:34:12Z 2006 journal article Development 133(19): 3755-3766 (2006) 0950-1991 http://hdl.handle.net/20.500.12792/4845 http://hdl.handle.net/10261/293989 10.1242/dev.02554 1477-9129 en open Company of Biologists |
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Arabidopsis TIP120 CAND1 Venation pattern formation Natural variation Arabidopsis TIP120 CAND1 Venation pattern formation Natural variation |
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Arabidopsis TIP120 CAND1 Venation pattern formation Natural variation Arabidopsis TIP120 CAND1 Venation pattern formation Natural variation Alonso-Peral, M. M. Candela, H. del Pozo, J. C. Martínez-Laborda, A. Ponce, M. R. Micol, J. L. The HVE/CAND1 gene is required for the early patterning of leaf venation in Arabidopsis |
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The hemivenata-1 (hve-1) recessive allele was isolated in a search for natural variations in the leaf venation pattern of Arabidopsis thaliana, where it was seen to cause extremely simple venation in vegetative leaves and cotyledons, increased shoot branching, and reduced root waving and fertility, traits that are reminiscent of some mutants deficient in auxin signaling. Reduced sensitivity to exogenous auxin was found in the hve-1 mutant, which otherwise displayed a wild-type response to auxin transport inhibitors. The HVE gene was positionally cloned and found to encode a CAND1 protein. The hve-1 mutation caused mis-splicing of the transcripts of the HVEICAND1 gene and a vein phenotype indistinguishable from that of hve-2 and hve-3, two putatively null T-DNA alleles. Inflorescence size and fertility were more affected by hve-2 and hve-3, suggesting that hve-1 is hypomorphic. The simple venation pattern of hve plants seems to arise from an early patterning defect. We found that HVE/CAND1 binds to CULLIN1, and that the venation patterns of axr1 and hve mutants are similar, which suggest that ubiquitin-mediated auxin signaling is required for venation patterning in laminar organs, the only exception being cauline leaves. Our analyses of double mutant and transgenic plants indicated that auxin transport and perception act independently to pattern leaf veins, and that the HVE/ CAND1 genes acts upstream of ATHB-8 at least in higher order veins, in a pathway that involves AXR1, but not LOP1, PIN1, CVP1 or CVP2. |
format |
journal article |
topic_facet |
Arabidopsis TIP120 CAND1 Venation pattern formation Natural variation |
author |
Alonso-Peral, M. M. Candela, H. del Pozo, J. C. Martínez-Laborda, A. Ponce, M. R. Micol, J. L. |
author_facet |
Alonso-Peral, M. M. Candela, H. del Pozo, J. C. Martínez-Laborda, A. Ponce, M. R. Micol, J. L. |
author_sort |
Alonso-Peral, M. M. |
title |
The HVE/CAND1 gene is required for the early patterning of leaf venation in Arabidopsis |
title_short |
The HVE/CAND1 gene is required for the early patterning of leaf venation in Arabidopsis |
title_full |
The HVE/CAND1 gene is required for the early patterning of leaf venation in Arabidopsis |
title_fullStr |
The HVE/CAND1 gene is required for the early patterning of leaf venation in Arabidopsis |
title_full_unstemmed |
The HVE/CAND1 gene is required for the early patterning of leaf venation in Arabidopsis |
title_sort |
hve/cand1 gene is required for the early patterning of leaf venation in arabidopsis |
publisher |
Company of Biologists |
publishDate |
2006 |
url |
http://hdl.handle.net/20.500.12792/4845 http://hdl.handle.net/10261/293989 |
work_keys_str_mv |
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