Fitness alteration of foot-and-mouth disease virus mutants Measurement of adaptability of viral quasispecies
We document the rapid alteration of fitness of two foot-and-mouth disease virus (FMDV) mutants resistant to a neutralizing monoclonal antibody. Both mutants showed a selective disadvantage in BHK-21 cells when passaged in competition with their parental FMDV. Upon repeated replication of the mutants alone, they acquired a selective advantage over the parental FMDV and fixed additional genomic substitutions without reversion of the monoclonal antibody-resistant phenotype. Thus, variants that were previously kept at low frequency in the mutant spectrum of a viral quasispecies rapidly became the master sequence of a new genomic distribution and dominated the viral population.
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American Society for Microbiology
1991
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Online Access: | http://hdl.handle.net/20.500.12792/2716 http://hdl.handle.net/10261/293777 |
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dig-inia-es-10261-2937772023-02-20T10:31:58Z Fitness alteration of foot-and-mouth disease virus mutants Measurement of adaptability of viral quasispecies Martinez, M. A. Carrillo García, Mª Cristina Gonzalez-Candelas, F. Moya, A. Domingo, E. Sobrino, F. We document the rapid alteration of fitness of two foot-and-mouth disease virus (FMDV) mutants resistant to a neutralizing monoclonal antibody. Both mutants showed a selective disadvantage in BHK-21 cells when passaged in competition with their parental FMDV. Upon repeated replication of the mutants alone, they acquired a selective advantage over the parental FMDV and fixed additional genomic substitutions without reversion of the monoclonal antibody-resistant phenotype. Thus, variants that were previously kept at low frequency in the mutant spectrum of a viral quasispecies rapidly became the master sequence of a new genomic distribution and dominated the viral population. 2023-02-20T10:31:58Z 2023-02-20T10:31:58Z 1991 journal article Journal of Virology 65(7): 3954-3957 (1991) 0022-538X http://hdl.handle.net/20.500.12792/2716 http://hdl.handle.net/10261/293777 1098-5514 en none American Society for Microbiology |
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We document the rapid alteration of fitness of two foot-and-mouth disease virus (FMDV) mutants resistant to a neutralizing monoclonal antibody. Both mutants showed a selective disadvantage in BHK-21 cells when passaged in competition with their parental FMDV. Upon repeated replication of the mutants alone, they acquired a selective advantage over the parental FMDV and fixed additional genomic substitutions without reversion of the monoclonal antibody-resistant phenotype. Thus, variants that were previously kept at low frequency in the mutant spectrum of a viral quasispecies rapidly became the master sequence of a new genomic distribution and dominated the viral population. |
format |
journal article |
author |
Martinez, M. A. Carrillo García, Mª Cristina Gonzalez-Candelas, F. Moya, A. Domingo, E. Sobrino, F. |
spellingShingle |
Martinez, M. A. Carrillo García, Mª Cristina Gonzalez-Candelas, F. Moya, A. Domingo, E. Sobrino, F. Fitness alteration of foot-and-mouth disease virus mutants Measurement of adaptability of viral quasispecies |
author_facet |
Martinez, M. A. Carrillo García, Mª Cristina Gonzalez-Candelas, F. Moya, A. Domingo, E. Sobrino, F. |
author_sort |
Martinez, M. A. |
title |
Fitness alteration of foot-and-mouth disease virus mutants Measurement of adaptability of viral quasispecies |
title_short |
Fitness alteration of foot-and-mouth disease virus mutants Measurement of adaptability of viral quasispecies |
title_full |
Fitness alteration of foot-and-mouth disease virus mutants Measurement of adaptability of viral quasispecies |
title_fullStr |
Fitness alteration of foot-and-mouth disease virus mutants Measurement of adaptability of viral quasispecies |
title_full_unstemmed |
Fitness alteration of foot-and-mouth disease virus mutants Measurement of adaptability of viral quasispecies |
title_sort |
fitness alteration of foot-and-mouth disease virus mutants measurement of adaptability of viral quasispecies |
publisher |
American Society for Microbiology |
publishDate |
1991 |
url |
http://hdl.handle.net/20.500.12792/2716 http://hdl.handle.net/10261/293777 |
work_keys_str_mv |
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