Fitness alteration of foot-and-mouth disease virus mutants Measurement of adaptability of viral quasispecies

Fitness alteration of foot-and-mouth disease virus mutants Measurement of adaptability of viral quasispecies

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We document the rapid alteration of fitness of two foot-and-mouth disease virus (FMDV) mutants resistant to a neutralizing monoclonal antibody. Both mutants showed a selective disadvantage in BHK-21 cells when passaged in competition with their parental FMDV. Upon repeated replication of the mutants alone, they acquired a selective advantage over the parental FMDV and fixed additional genomic substitutions without reversion of the monoclonal antibody-resistant phenotype. Thus, variants that were previously kept at low frequency in the mutant spectrum of a viral quasispecies rapidly became the master sequence of a new genomic distribution and dominated the viral population.

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Main Authors: Martinez, M. A., Carrillo García, Mª Cristina, Gonzalez-Candelas, F., Moya, A., Domingo, E., Sobrino, F.
Format: journal article biblioteca
Language:English
Published: American Society for Microbiology 1991
Online Access:http://hdl.handle.net/20.500.12792/2716
http://hdl.handle.net/10261/293777
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spelling dig-inia-es-10261-2937772023-02-20T10:31:58Z Fitness alteration of foot-and-mouth disease virus mutants Measurement of adaptability of viral quasispecies Martinez, M. A. Carrillo García, Mª Cristina Gonzalez-Candelas, F. Moya, A. Domingo, E. Sobrino, F. We document the rapid alteration of fitness of two foot-and-mouth disease virus (FMDV) mutants resistant to a neutralizing monoclonal antibody. Both mutants showed a selective disadvantage in BHK-21 cells when passaged in competition with their parental FMDV. Upon repeated replication of the mutants alone, they acquired a selective advantage over the parental FMDV and fixed additional genomic substitutions without reversion of the monoclonal antibody-resistant phenotype. Thus, variants that were previously kept at low frequency in the mutant spectrum of a viral quasispecies rapidly became the master sequence of a new genomic distribution and dominated the viral population. 2023-02-20T10:31:58Z 2023-02-20T10:31:58Z 1991 journal article Journal of Virology 65(7): 3954-3957 (1991) 0022-538X http://hdl.handle.net/20.500.12792/2716 http://hdl.handle.net/10261/293777 1098-5514 en none American Society for Microbiology
institution INIA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-inia-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del INIA España
language English
description We document the rapid alteration of fitness of two foot-and-mouth disease virus (FMDV) mutants resistant to a neutralizing monoclonal antibody. Both mutants showed a selective disadvantage in BHK-21 cells when passaged in competition with their parental FMDV. Upon repeated replication of the mutants alone, they acquired a selective advantage over the parental FMDV and fixed additional genomic substitutions without reversion of the monoclonal antibody-resistant phenotype. Thus, variants that were previously kept at low frequency in the mutant spectrum of a viral quasispecies rapidly became the master sequence of a new genomic distribution and dominated the viral population.
format journal article
author Martinez, M. A.
Carrillo García, Mª Cristina
Gonzalez-Candelas, F.
Moya, A.
Domingo, E.
Sobrino, F.
spellingShingle Martinez, M. A.
Carrillo García, Mª Cristina
Gonzalez-Candelas, F.
Moya, A.
Domingo, E.
Sobrino, F.
Fitness alteration of foot-and-mouth disease virus mutants Measurement of adaptability of viral quasispecies
author_facet Martinez, M. A.
Carrillo García, Mª Cristina
Gonzalez-Candelas, F.
Moya, A.
Domingo, E.
Sobrino, F.
author_sort Martinez, M. A.
title Fitness alteration of foot-and-mouth disease virus mutants Measurement of adaptability of viral quasispecies
title_short Fitness alteration of foot-and-mouth disease virus mutants Measurement of adaptability of viral quasispecies
title_full Fitness alteration of foot-and-mouth disease virus mutants Measurement of adaptability of viral quasispecies
title_fullStr Fitness alteration of foot-and-mouth disease virus mutants Measurement of adaptability of viral quasispecies
title_full_unstemmed Fitness alteration of foot-and-mouth disease virus mutants Measurement of adaptability of viral quasispecies
title_sort fitness alteration of foot-and-mouth disease virus mutants measurement of adaptability of viral quasispecies
publisher American Society for Microbiology
publishDate 1991
url http://hdl.handle.net/20.500.12792/2716
http://hdl.handle.net/10261/293777
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