Microspheres-prime/rMVA-boost vaccination enhances humoral and cellular immune response in IFNAR(−/−) mice conferring protection against serotypes 1 and 4 of bluetongue virus
Bluetongue virus (BTV) is the causative agent of bluetongue disease (BT), which affects domestic and wild ruminants. At the present, 27 different serotypes have been documented. Vaccination has been demonstrated as one of the most effective methods to avoid viral dissemination. To overcome the drawbacks associated with the use of inactivated and attenuated vaccines we engineered a new recombinant BTV vaccine candidate based on proteins VP2, VP7, and NS1 of BTV-4 that were incorporated into avian reovirus muNS-Mi microspheres (MS-VP2/VP7/NS1) and recombinant modified vaccinia virus Ankara (rMVA). The combination of these two antigen delivery systems in a heterologous prime-boost vaccination strategy generated significant levels of neutralizing antibodies in IFNAR(−/−) mice. Furthermore, this immunization strategy increased the ratio of IgG2a/IgG1 in sera, indicating an induction of a Th1 response, and elicited a CD8 T cell response. Immunized mice were protected against lethal challenges with the homologous serotype 4 and the heterologous serotype 1 of BTV. All these results support the strategy based on microspheres in combination with rMVAs as a promising multiserotype vaccine candidate against BTV. © 2017 Elsevier B.V.
Main Authors: | , , , , , , , |
---|---|
Format: | journal article biblioteca |
Language: | English |
Published: |
Elsevier
2017
|
Subjects: | Bluetongue, Microspheres, MVA, Multiserotype, Vaccine, |
Online Access: | http://hdl.handle.net/20.500.12792/2631 http://hdl.handle.net/10261/293747 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-inia-es-10261-293747 |
---|---|
record_format |
koha |
spelling |
dig-inia-es-10261-2937472023-02-20T10:31:37Z Microspheres-prime/rMVA-boost vaccination enhances humoral and cellular immune response in IFNAR(−/−) mice conferring protection against serotypes 1 and 4 of bluetongue virus Marín-López, A. Calvo Pinilla, Eva María Barriales, D. Lorenzo Alguacil, Gema Benavente, J. Brun Torres, Alejandro Martínez-Costas, J. M. Ortego Alonso, Francisco Javier Bluetongue Microspheres MVA Multiserotype Vaccine Bluetongue virus (BTV) is the causative agent of bluetongue disease (BT), which affects domestic and wild ruminants. At the present, 27 different serotypes have been documented. Vaccination has been demonstrated as one of the most effective methods to avoid viral dissemination. To overcome the drawbacks associated with the use of inactivated and attenuated vaccines we engineered a new recombinant BTV vaccine candidate based on proteins VP2, VP7, and NS1 of BTV-4 that were incorporated into avian reovirus muNS-Mi microspheres (MS-VP2/VP7/NS1) and recombinant modified vaccinia virus Ankara (rMVA). The combination of these two antigen delivery systems in a heterologous prime-boost vaccination strategy generated significant levels of neutralizing antibodies in IFNAR(−/−) mice. Furthermore, this immunization strategy increased the ratio of IgG2a/IgG1 in sera, indicating an induction of a Th1 response, and elicited a CD8 T cell response. Immunized mice were protected against lethal challenges with the homologous serotype 4 and the heterologous serotype 1 of BTV. All these results support the strategy based on microspheres in combination with rMVAs as a promising multiserotype vaccine candidate against BTV. © 2017 Elsevier B.V. 2023-02-20T10:31:37Z 2023-02-20T10:31:37Z 2017 journal article Antiviral Research 142: 55 - 62 (2017) 0166-3542 http://hdl.handle.net/20.500.12792/2631 http://hdl.handle.net/10261/293747 10.1016/j.antiviral.2017.03.010 1872-9096 en none Elsevier |
institution |
INIA ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-inia-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del INIA España |
language |
English |
topic |
Bluetongue Microspheres MVA Multiserotype Vaccine Bluetongue Microspheres MVA Multiserotype Vaccine |
spellingShingle |
Bluetongue Microspheres MVA Multiserotype Vaccine Bluetongue Microspheres MVA Multiserotype Vaccine Marín-López, A. Calvo Pinilla, Eva María Barriales, D. Lorenzo Alguacil, Gema Benavente, J. Brun Torres, Alejandro Martínez-Costas, J. M. Ortego Alonso, Francisco Javier Microspheres-prime/rMVA-boost vaccination enhances humoral and cellular immune response in IFNAR(−/−) mice conferring protection against serotypes 1 and 4 of bluetongue virus |
description |
Bluetongue virus (BTV) is the causative agent of bluetongue disease (BT), which affects domestic and wild ruminants. At the present, 27 different serotypes have been documented. Vaccination has been demonstrated as one of the most effective methods to avoid viral dissemination. To overcome the drawbacks associated with the use of inactivated and attenuated vaccines we engineered a new recombinant BTV vaccine candidate based on proteins VP2, VP7, and NS1 of BTV-4 that were incorporated into avian reovirus muNS-Mi microspheres (MS-VP2/VP7/NS1) and recombinant modified vaccinia virus Ankara (rMVA). The combination of these two antigen delivery systems in a heterologous prime-boost vaccination strategy generated significant levels of neutralizing antibodies in IFNAR(−/−) mice. Furthermore, this immunization strategy increased the ratio of IgG2a/IgG1 in sera, indicating an induction of a Th1 response, and elicited a CD8 T cell response. Immunized mice were protected against lethal challenges with the homologous serotype 4 and the heterologous serotype 1 of BTV. All these results support the strategy based on microspheres in combination with rMVAs as a promising multiserotype vaccine candidate against BTV. © 2017 Elsevier B.V. |
format |
journal article |
topic_facet |
Bluetongue Microspheres MVA Multiserotype Vaccine |
author |
Marín-López, A. Calvo Pinilla, Eva María Barriales, D. Lorenzo Alguacil, Gema Benavente, J. Brun Torres, Alejandro Martínez-Costas, J. M. Ortego Alonso, Francisco Javier |
author_facet |
Marín-López, A. Calvo Pinilla, Eva María Barriales, D. Lorenzo Alguacil, Gema Benavente, J. Brun Torres, Alejandro Martínez-Costas, J. M. Ortego Alonso, Francisco Javier |
author_sort |
Marín-López, A. |
title |
Microspheres-prime/rMVA-boost vaccination enhances humoral and cellular immune response in IFNAR(−/−) mice conferring protection against serotypes 1 and 4 of bluetongue virus |
title_short |
Microspheres-prime/rMVA-boost vaccination enhances humoral and cellular immune response in IFNAR(−/−) mice conferring protection against serotypes 1 and 4 of bluetongue virus |
title_full |
Microspheres-prime/rMVA-boost vaccination enhances humoral and cellular immune response in IFNAR(−/−) mice conferring protection against serotypes 1 and 4 of bluetongue virus |
title_fullStr |
Microspheres-prime/rMVA-boost vaccination enhances humoral and cellular immune response in IFNAR(−/−) mice conferring protection against serotypes 1 and 4 of bluetongue virus |
title_full_unstemmed |
Microspheres-prime/rMVA-boost vaccination enhances humoral and cellular immune response in IFNAR(−/−) mice conferring protection against serotypes 1 and 4 of bluetongue virus |
title_sort |
microspheres-prime/rmva-boost vaccination enhances humoral and cellular immune response in ifnar(−/−) mice conferring protection against serotypes 1 and 4 of bluetongue virus |
publisher |
Elsevier |
publishDate |
2017 |
url |
http://hdl.handle.net/20.500.12792/2631 http://hdl.handle.net/10261/293747 |
work_keys_str_mv |
AT marinlopeza microspheresprimermvaboostvaccinationenhanceshumoralandcellularimmuneresponseinifnarmiceconferringprotectionagainstserotypes1and4ofbluetonguevirus AT calvopinillaevamaria microspheresprimermvaboostvaccinationenhanceshumoralandcellularimmuneresponseinifnarmiceconferringprotectionagainstserotypes1and4ofbluetonguevirus AT barrialesd microspheresprimermvaboostvaccinationenhanceshumoralandcellularimmuneresponseinifnarmiceconferringprotectionagainstserotypes1and4ofbluetonguevirus AT lorenzoalguacilgema microspheresprimermvaboostvaccinationenhanceshumoralandcellularimmuneresponseinifnarmiceconferringprotectionagainstserotypes1and4ofbluetonguevirus AT benaventej microspheresprimermvaboostvaccinationenhanceshumoralandcellularimmuneresponseinifnarmiceconferringprotectionagainstserotypes1and4ofbluetonguevirus AT bruntorresalejandro microspheresprimermvaboostvaccinationenhanceshumoralandcellularimmuneresponseinifnarmiceconferringprotectionagainstserotypes1and4ofbluetonguevirus AT martinezcostasjm microspheresprimermvaboostvaccinationenhanceshumoralandcellularimmuneresponseinifnarmiceconferringprotectionagainstserotypes1and4ofbluetonguevirus AT ortegoalonsofranciscojavier microspheresprimermvaboostvaccinationenhanceshumoralandcellularimmuneresponseinifnarmiceconferringprotectionagainstserotypes1and4ofbluetonguevirus |
_version_ |
1767603518612439040 |