Trout oral VP2 DNA vaccination mimics transcriptional responses occurring after infection with infectious pancreatic necrosis virus (IPNV)
Time-course and organ transcriptional response profiles in rainbow trout Oncorhynchus mykiss were studied after oral DNA-vaccination with the VP2 gene of the infectious pancreatic necrosis virus (IPNV) encapsulated in alginates. The profiles were also compared with those obtained after infection with IPNV. A group of immune-related genes (stat1, ifn1, ifng, mx1, mx3, il8, il10, il11, il12b, tnf2, mhc1uda, igm and igt) previously selected from microarray analysis of successful oral vaccination of rainbow trout, were used for the RTqPCR analysis. The results showed that oral VP2-vaccination qualitatively mimicked both the time-course and organ (head kidney, spleen, intestine, pyloric ceca, and thymus) transcriptional profiles obtained after IPNV-infection. Highest transcriptional differential expression levels after oral vaccination were obtained in thymus, suggesting those might be important for subsequent protection against IPNV challenges. However, transcriptional differential expression levels of most of the genes mentioned above were lower in VP2-vaccinated than in IPNV-infected trout, except for ifn1 which were similar. Together all the results suggest that the oral-alginate VP2-vaccination procedure immunizes trout against IPNV in a similar way as IPNV-infection does while there is still room for additional improvements in the oral vaccination procedure. Some of the genes described here could be used as markers to further optimize the oral immunization method. © 2012 Elsevier Ltd.
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Format: | journal article biblioteca |
Language: | English |
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Elsevier
2012
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Subjects: | Infectious pancreatic necrosis virus, VP2, Oral DNA vaccines, Trout, IPNV, |
Online Access: | http://hdl.handle.net/20.500.12792/2641 http://hdl.handle.net/10261/293611 |
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dig-inia-es-10261-2936112023-02-20T10:30:14Z Trout oral VP2 DNA vaccination mimics transcriptional responses occurring after infection with infectious pancreatic necrosis virus (IPNV) Ballesteros, N. A. Saint-Jean, S. S. R. Perez-Prieto, S. I. Coll, J. M. Infectious pancreatic necrosis virus VP2 Oral DNA vaccines Trout IPNV Time-course and organ transcriptional response profiles in rainbow trout Oncorhynchus mykiss were studied after oral DNA-vaccination with the VP2 gene of the infectious pancreatic necrosis virus (IPNV) encapsulated in alginates. The profiles were also compared with those obtained after infection with IPNV. A group of immune-related genes (stat1, ifn1, ifng, mx1, mx3, il8, il10, il11, il12b, tnf2, mhc1uda, igm and igt) previously selected from microarray analysis of successful oral vaccination of rainbow trout, were used for the RTqPCR analysis. The results showed that oral VP2-vaccination qualitatively mimicked both the time-course and organ (head kidney, spleen, intestine, pyloric ceca, and thymus) transcriptional profiles obtained after IPNV-infection. Highest transcriptional differential expression levels after oral vaccination were obtained in thymus, suggesting those might be important for subsequent protection against IPNV challenges. However, transcriptional differential expression levels of most of the genes mentioned above were lower in VP2-vaccinated than in IPNV-infected trout, except for ifn1 which were similar. Together all the results suggest that the oral-alginate VP2-vaccination procedure immunizes trout against IPNV in a similar way as IPNV-infection does while there is still room for additional improvements in the oral vaccination procedure. Some of the genes described here could be used as markers to further optimize the oral immunization method. © 2012 Elsevier Ltd. 2023-02-20T10:30:14Z 2023-02-20T10:30:14Z 2012 journal article Fish and Shellfish Immunology 33(6): 1249-1257 (2012) 1050-4648 http://hdl.handle.net/20.500.12792/2641 http://hdl.handle.net/10261/293611 10.1016/j.fsi.2012.09.004 en none Elsevier |
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Infectious pancreatic necrosis virus VP2 Oral DNA vaccines Trout IPNV Infectious pancreatic necrosis virus VP2 Oral DNA vaccines Trout IPNV |
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Infectious pancreatic necrosis virus VP2 Oral DNA vaccines Trout IPNV Infectious pancreatic necrosis virus VP2 Oral DNA vaccines Trout IPNV Ballesteros, N. A. Saint-Jean, S. S. R. Perez-Prieto, S. I. Coll, J. M. Trout oral VP2 DNA vaccination mimics transcriptional responses occurring after infection with infectious pancreatic necrosis virus (IPNV) |
description |
Time-course and organ transcriptional response profiles in rainbow trout Oncorhynchus mykiss were studied after oral DNA-vaccination with the VP2 gene of the infectious pancreatic necrosis virus (IPNV) encapsulated in alginates. The profiles were also compared with those obtained after infection with IPNV. A group of immune-related genes (stat1, ifn1, ifng, mx1, mx3, il8, il10, il11, il12b, tnf2, mhc1uda, igm and igt) previously selected from microarray analysis of successful oral vaccination of rainbow trout, were used for the RTqPCR analysis. The results showed that oral VP2-vaccination qualitatively mimicked both the time-course and organ (head kidney, spleen, intestine, pyloric ceca, and thymus) transcriptional profiles obtained after IPNV-infection. Highest transcriptional differential expression levels after oral vaccination were obtained in thymus, suggesting those might be important for subsequent protection against IPNV challenges. However, transcriptional differential expression levels of most of the genes mentioned above were lower in VP2-vaccinated than in IPNV-infected trout, except for ifn1 which were similar. Together all the results suggest that the oral-alginate VP2-vaccination procedure immunizes trout against IPNV in a similar way as IPNV-infection does while there is still room for additional improvements in the oral vaccination procedure. Some of the genes described here could be used as markers to further optimize the oral immunization method. © 2012 Elsevier Ltd. |
format |
journal article |
topic_facet |
Infectious pancreatic necrosis virus VP2 Oral DNA vaccines Trout IPNV |
author |
Ballesteros, N. A. Saint-Jean, S. S. R. Perez-Prieto, S. I. Coll, J. M. |
author_facet |
Ballesteros, N. A. Saint-Jean, S. S. R. Perez-Prieto, S. I. Coll, J. M. |
author_sort |
Ballesteros, N. A. |
title |
Trout oral VP2 DNA vaccination mimics transcriptional responses occurring after infection with infectious pancreatic necrosis virus (IPNV) |
title_short |
Trout oral VP2 DNA vaccination mimics transcriptional responses occurring after infection with infectious pancreatic necrosis virus (IPNV) |
title_full |
Trout oral VP2 DNA vaccination mimics transcriptional responses occurring after infection with infectious pancreatic necrosis virus (IPNV) |
title_fullStr |
Trout oral VP2 DNA vaccination mimics transcriptional responses occurring after infection with infectious pancreatic necrosis virus (IPNV) |
title_full_unstemmed |
Trout oral VP2 DNA vaccination mimics transcriptional responses occurring after infection with infectious pancreatic necrosis virus (IPNV) |
title_sort |
trout oral vp2 dna vaccination mimics transcriptional responses occurring after infection with infectious pancreatic necrosis virus (ipnv) |
publisher |
Elsevier |
publishDate |
2012 |
url |
http://hdl.handle.net/20.500.12792/2641 http://hdl.handle.net/10261/293611 |
work_keys_str_mv |
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_version_ |
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