Crystallization and preliminary X-ray analysis of swine vesicular disease virus (SVDV)
Three different crystal forms of the swine vesicular disease virus (SVDV), isolate SPA/2/'93, were obtained by the hanging-drop vapour-diffusion technique using ammonium sulfate and sodium/potassium phosphate as precipitants. Monoclinic crystals, space group C2, with unit-cell parameters a = 473.7, b = 385.3, c = 472.8 Å, β = 100.4°, contain one virus particle in the crystal asymmetric unit and diffract to 3.0 Å resolution. A second type of crystals had a cubic morphology and diffracted beyond 2.6 Å resolution. These crystals belong to a primitive orthorhombic space group, with unit-cell parameters a = 319.6, b = 353.8, c = 377.7 Å, and contain half a virus particle in the asymmetric unit. A third type of crystals, with a prismatic shape and belonging to space group I222, was also obtained under similar crystallization conditions. These latter crystals, with unit-cell parameters a = 318.3, b = 349.9, c = 371.7 Å, diffract to at least 3.0 Å resolution and contain 15 protomers per asymmetric unit; this requires that three perpendicular crystal twofold axes coincide with three of the viral particles' dyad axes.
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2003
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dig-inia-es-10261-2935722023-02-20T10:29:52Z Crystallization and preliminary X-ray analysis of swine vesicular disease virus (SVDV) Jimenez-Clavero, M. A. Ley, V. Fita, I. Verdaguer, N. Three different crystal forms of the swine vesicular disease virus (SVDV), isolate SPA/2/'93, were obtained by the hanging-drop vapour-diffusion technique using ammonium sulfate and sodium/potassium phosphate as precipitants. Monoclinic crystals, space group C2, with unit-cell parameters a = 473.7, b = 385.3, c = 472.8 Å, β = 100.4°, contain one virus particle in the crystal asymmetric unit and diffract to 3.0 Å resolution. A second type of crystals had a cubic morphology and diffracted beyond 2.6 Å resolution. These crystals belong to a primitive orthorhombic space group, with unit-cell parameters a = 319.6, b = 353.8, c = 377.7 Å, and contain half a virus particle in the asymmetric unit. A third type of crystals, with a prismatic shape and belonging to space group I222, was also obtained under similar crystallization conditions. These latter crystals, with unit-cell parameters a = 318.3, b = 349.9, c = 371.7 Å, diffract to at least 3.0 Å resolution and contain 15 protomers per asymmetric unit; this requires that three perpendicular crystal twofold axes coincide with three of the viral particles' dyad axes. 2023-02-20T10:29:52Z 2023-02-20T10:29:52Z 2003 journal article Acta Crystallographica Section D: Biological Crystallography 59(3): 541-543 (2003) 2059-7983 http://hdl.handle.net/20.500.12792/2213 http://hdl.handle.net/10261/293572 10.1107/S0907444902023648 en open Wiley |
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Three different crystal forms of the swine vesicular disease virus (SVDV), isolate SPA/2/'93, were obtained by the hanging-drop vapour-diffusion technique using ammonium sulfate and sodium/potassium phosphate as precipitants. Monoclinic crystals, space group C2, with unit-cell parameters a = 473.7, b = 385.3, c = 472.8 Å, β = 100.4°, contain one virus particle in the crystal asymmetric unit and diffract to 3.0 Å resolution. A second type of crystals had a cubic morphology and diffracted beyond 2.6 Å resolution. These crystals belong to a primitive orthorhombic space group, with unit-cell parameters a = 319.6, b = 353.8, c = 377.7 Å, and contain half a virus particle in the asymmetric unit. A third type of crystals, with a prismatic shape and belonging to space group I222, was also obtained under similar crystallization conditions. These latter crystals, with unit-cell parameters a = 318.3, b = 349.9, c = 371.7 Å, diffract to at least 3.0 Å resolution and contain 15 protomers per asymmetric unit; this requires that three perpendicular crystal twofold axes coincide with three of the viral particles' dyad axes. |
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journal article |
author |
Jimenez-Clavero, M. A. Ley, V. Fita, I. Verdaguer, N. |
spellingShingle |
Jimenez-Clavero, M. A. Ley, V. Fita, I. Verdaguer, N. Crystallization and preliminary X-ray analysis of swine vesicular disease virus (SVDV) |
author_facet |
Jimenez-Clavero, M. A. Ley, V. Fita, I. Verdaguer, N. |
author_sort |
Jimenez-Clavero, M. A. |
title |
Crystallization and preliminary X-ray analysis of swine vesicular disease virus (SVDV) |
title_short |
Crystallization and preliminary X-ray analysis of swine vesicular disease virus (SVDV) |
title_full |
Crystallization and preliminary X-ray analysis of swine vesicular disease virus (SVDV) |
title_fullStr |
Crystallization and preliminary X-ray analysis of swine vesicular disease virus (SVDV) |
title_full_unstemmed |
Crystallization and preliminary X-ray analysis of swine vesicular disease virus (SVDV) |
title_sort |
crystallization and preliminary x-ray analysis of swine vesicular disease virus (svdv) |
publisher |
Wiley |
publishDate |
2003 |
url |
http://hdl.handle.net/20.500.12792/2213 http://hdl.handle.net/10261/293572 |
work_keys_str_mv |
AT jimenezclaveroma crystallizationandpreliminaryxrayanalysisofswinevesiculardiseasevirussvdv AT leyv crystallizationandpreliminaryxrayanalysisofswinevesiculardiseasevirussvdv AT fitai crystallizationandpreliminaryxrayanalysisofswinevesiculardiseasevirussvdv AT verdaguern crystallizationandpreliminaryxrayanalysisofswinevesiculardiseasevirussvdv |
_version_ |
1767603494454296576 |