Restoration of endocrine and ovarian function after stopping GnRH antagonist treatment in goats
We have tested if the high number of unfertilized ova and degenerated embryos found in superovulated goats previously treated with GnRH antagonist can be related to a prolongation of gonadotrophin down-regulation and/or alterations in follicular function during the period of administration of the superovulatory treatment, around 4 days after the end of the antagonist treatment. A total of 15 does were treated with intravaginal progestagen sponges and daily injections of 0.5 mg of the GnRH antagonist Antarelix™ for 6 days, while 5 does acted as controls receiving saline. During the antagonist treatment, the mean plasma LH concentration was lower in treated than control goats (0.5±0.2 versus 0.7±0.5 ng/ml, P<0.0005); however, the FSH levels remained unaffected (0.8±0.4 versus 0.8±0.5 ng/ml). In this period, treated does also showed an increase in the number of small follicles with 2-3 mm in size (10.7±0.7 versus 8.4±0.6, P<0.05), and a decrease in both the number of follicles ≥4 mm in size (5.0±0.3 versus 6.8±0.5, P<0.005) and the secretion of inhibin A (120.9±10.7 versus 151.6±12.6 pg/ml, P<0.05). After cessation of the antagonist treatment, there was an increase in LH levels in treated goats from the day after the last Antarelix™ injection (Day 1), so that LH levels were the same as controls on Day 3 (0.6 ± 0.1 versus 0.6 ± 0.2 ng/ml). However, there were even greater numbers of small follicles than during the period of antagonist injections (15.4 ± 0.6 in treated versus 8.9 ± 0.7 in control, P < 0.0005). Moreover, the number of ≥4 mm follicles and the secretion of inhibin A remained lower in treated goats (3.9 ± 0.3 follicles and 84.4 ± 7.0 pg/ml versus 5.4 ± 0.5 follicles, P < 0.05 and 128.9 ± 14.2 pg/ml, P < 0.05). These results indicate that pituitary secretion of gonadotrophins is restored shortly after the end of antagonist treatment, but activity of ovarian follicles is affected. © 2004 Elsevier Inc. All rights reserved.
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Elsevier
2005
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Subjects: | Follicle, GnRH analogues, Goat, Gonadotrophins, Superovulation, |
Online Access: | http://hdl.handle.net/20.500.12792/1906 http://hdl.handle.net/10261/293504 |
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dig-inia-es-10261-2935042023-02-20T10:29:07Z Restoration of endocrine and ovarian function after stopping GnRH antagonist treatment in goats González De Bulnes, Antonio López Sebastián, Antonio Garcia-Garcia, R. M. Veiga-Lopez, A. Souza, C. J. H. McNeilly, A. S. Follicle GnRH analogues Goat Gonadotrophins Superovulation We have tested if the high number of unfertilized ova and degenerated embryos found in superovulated goats previously treated with GnRH antagonist can be related to a prolongation of gonadotrophin down-regulation and/or alterations in follicular function during the period of administration of the superovulatory treatment, around 4 days after the end of the antagonist treatment. A total of 15 does were treated with intravaginal progestagen sponges and daily injections of 0.5 mg of the GnRH antagonist Antarelix™ for 6 days, while 5 does acted as controls receiving saline. During the antagonist treatment, the mean plasma LH concentration was lower in treated than control goats (0.5±0.2 versus 0.7±0.5 ng/ml, P<0.0005); however, the FSH levels remained unaffected (0.8±0.4 versus 0.8±0.5 ng/ml). In this period, treated does also showed an increase in the number of small follicles with 2-3 mm in size (10.7±0.7 versus 8.4±0.6, P<0.05), and a decrease in both the number of follicles ≥4 mm in size (5.0±0.3 versus 6.8±0.5, P<0.005) and the secretion of inhibin A (120.9±10.7 versus 151.6±12.6 pg/ml, P<0.05). After cessation of the antagonist treatment, there was an increase in LH levels in treated goats from the day after the last Antarelix™ injection (Day 1), so that LH levels were the same as controls on Day 3 (0.6 ± 0.1 versus 0.6 ± 0.2 ng/ml). However, there were even greater numbers of small follicles than during the period of antagonist injections (15.4 ± 0.6 in treated versus 8.9 ± 0.7 in control, P < 0.0005). Moreover, the number of ≥4 mm follicles and the secretion of inhibin A remained lower in treated goats (3.9 ± 0.3 follicles and 84.4 ± 7.0 pg/ml versus 5.4 ± 0.5 follicles, P < 0.05 and 128.9 ± 14.2 pg/ml, P < 0.05). These results indicate that pituitary secretion of gonadotrophins is restored shortly after the end of antagonist treatment, but activity of ovarian follicles is affected. © 2004 Elsevier Inc. All rights reserved. 2023-02-20T10:29:07Z 2023-02-20T10:29:07Z 2005 journal article Theriogenology 63(1): 83-91 (2005) 0093-691X http://hdl.handle.net/20.500.12792/1906 http://hdl.handle.net/10261/293504 10.1016/j.theriogenology.2004.03.019 1879-3231 en none Elsevier |
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Follicle GnRH analogues Goat Gonadotrophins Superovulation Follicle GnRH analogues Goat Gonadotrophins Superovulation |
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Follicle GnRH analogues Goat Gonadotrophins Superovulation Follicle GnRH analogues Goat Gonadotrophins Superovulation González De Bulnes, Antonio López Sebastián, Antonio Garcia-Garcia, R. M. Veiga-Lopez, A. Souza, C. J. H. McNeilly, A. S. Restoration of endocrine and ovarian function after stopping GnRH antagonist treatment in goats |
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We have tested if the high number of unfertilized ova and degenerated embryos found in superovulated goats previously treated with GnRH antagonist can be related to a prolongation of gonadotrophin down-regulation and/or alterations in follicular function during the period of administration of the superovulatory treatment, around 4 days after the end of the antagonist treatment. A total of 15 does were treated with intravaginal progestagen sponges and daily injections of 0.5 mg of the GnRH antagonist Antarelix™ for 6 days, while 5 does acted as controls receiving saline. During the antagonist treatment, the mean plasma LH concentration was lower in treated than control goats (0.5±0.2 versus 0.7±0.5 ng/ml, P<0.0005); however, the FSH levels remained unaffected (0.8±0.4 versus 0.8±0.5 ng/ml). In this period, treated does also showed an increase in the number of small follicles with 2-3 mm in size (10.7±0.7 versus 8.4±0.6, P<0.05), and a decrease in both the number of follicles ≥4 mm in size (5.0±0.3 versus 6.8±0.5, P<0.005) and the secretion of inhibin A (120.9±10.7 versus 151.6±12.6 pg/ml, P<0.05). After cessation of the antagonist treatment, there was an increase in LH levels in treated goats from the day after the last Antarelix™ injection (Day 1), so that LH levels were the same as controls on Day 3 (0.6 ± 0.1 versus 0.6 ± 0.2 ng/ml). However, there were even greater numbers of small follicles than during the period of antagonist injections (15.4 ± 0.6 in treated versus 8.9 ± 0.7 in control, P < 0.0005). Moreover, the number of ≥4 mm follicles and the secretion of inhibin A remained lower in treated goats (3.9 ± 0.3 follicles and 84.4 ± 7.0 pg/ml versus 5.4 ± 0.5 follicles, P < 0.05 and 128.9 ± 14.2 pg/ml, P < 0.05). These results indicate that pituitary secretion of gonadotrophins is restored shortly after the end of antagonist treatment, but activity of ovarian follicles is affected. © 2004 Elsevier Inc. All rights reserved. |
format |
journal article |
topic_facet |
Follicle GnRH analogues Goat Gonadotrophins Superovulation |
author |
González De Bulnes, Antonio López Sebastián, Antonio Garcia-Garcia, R. M. Veiga-Lopez, A. Souza, C. J. H. McNeilly, A. S. |
author_facet |
González De Bulnes, Antonio López Sebastián, Antonio Garcia-Garcia, R. M. Veiga-Lopez, A. Souza, C. J. H. McNeilly, A. S. |
author_sort |
González De Bulnes, Antonio |
title |
Restoration of endocrine and ovarian function after stopping GnRH antagonist treatment in goats |
title_short |
Restoration of endocrine and ovarian function after stopping GnRH antagonist treatment in goats |
title_full |
Restoration of endocrine and ovarian function after stopping GnRH antagonist treatment in goats |
title_fullStr |
Restoration of endocrine and ovarian function after stopping GnRH antagonist treatment in goats |
title_full_unstemmed |
Restoration of endocrine and ovarian function after stopping GnRH antagonist treatment in goats |
title_sort |
restoration of endocrine and ovarian function after stopping gnrh antagonist treatment in goats |
publisher |
Elsevier |
publishDate |
2005 |
url |
http://hdl.handle.net/20.500.12792/1906 http://hdl.handle.net/10261/293504 |
work_keys_str_mv |
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_version_ |
1767603485071638528 |