Dimethyleacetamide improves the cryosurvivability of Indian red jungle fowl (Gallus gallus murghi) sperm
It was hypothesized that dimethyleacetamide (DMA) can be used as an alternate to glycerol for cryopreservation of Indian red jungle fowl semen. Four concentrations of DMA (4%, 6%, 8% and 10%) in extender were compared with previously optimized cryopreservation protocol based on 20% glycerol (control) for Indian red jungle fowl. Sperm motility, plasma membrane integrity, viability, and acrosome integrity were assessed at the stage of post-dilution, cooling, equilibration, and freeze-thawing. The whole experiment was repeated/replicated for five times independently. Sperm motility, plasma membrane integrity, viability and acrosome integrity were recorded highest (P < 0.05) at post-dilution, cooling, equilibration, and freeze-thawing in extender having 6% DMA compared to control and other experimental extenders. The highest (P < 0.05) recovery rates of all aforementioned parameters were also recorded in extender having 6% DMA; thus, 6% DMA was further compared with control (20% glycerol) for fertility after artificial insemination. Eggs were collected for five days after artificial insemination with semen cryopreserved in extender containing 6% DMA and control. The higher no. of fertilized eggs, fertility, no. of hatched eggs, hatch (%) and hatchability were recorded with semen cryopreserved in extender having 6% DMA compared to control. It is concluded that 6% DMA maintained higher post-thaw quality and fertility of Indian red jungle fowl semen and is a better replacement of glycerol. © 2017
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Format: | journal article biblioteca |
Language: | English |
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Elsevier
2017
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Subjects: | Cryo-damage, Motility, Fertility, Acrosome, Sperm viability, Indian red jungle fowl, |
Online Access: | http://hdl.handle.net/20.500.12792/3615 http://hdl.handle.net/10261/293445 |
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dig-inia-es-10261-2934452023-02-20T10:28:34Z Dimethyleacetamide improves the cryosurvivability of Indian red jungle fowl (Gallus gallus murghi) sperm Rakha, B. A. Ansari, M. S. Akhter, S. Zafar, Z. Naseer, A. Hussain, I. Santiago Moreno, Julián Blesbois, E. Cryo-damage Motility Fertility Acrosome Sperm viability Indian red jungle fowl It was hypothesized that dimethyleacetamide (DMA) can be used as an alternate to glycerol for cryopreservation of Indian red jungle fowl semen. Four concentrations of DMA (4%, 6%, 8% and 10%) in extender were compared with previously optimized cryopreservation protocol based on 20% glycerol (control) for Indian red jungle fowl. Sperm motility, plasma membrane integrity, viability, and acrosome integrity were assessed at the stage of post-dilution, cooling, equilibration, and freeze-thawing. The whole experiment was repeated/replicated for five times independently. Sperm motility, plasma membrane integrity, viability and acrosome integrity were recorded highest (P < 0.05) at post-dilution, cooling, equilibration, and freeze-thawing in extender having 6% DMA compared to control and other experimental extenders. The highest (P < 0.05) recovery rates of all aforementioned parameters were also recorded in extender having 6% DMA; thus, 6% DMA was further compared with control (20% glycerol) for fertility after artificial insemination. Eggs were collected for five days after artificial insemination with semen cryopreserved in extender containing 6% DMA and control. The higher no. of fertilized eggs, fertility, no. of hatched eggs, hatch (%) and hatchability were recorded with semen cryopreserved in extender having 6% DMA compared to control. It is concluded that 6% DMA maintained higher post-thaw quality and fertility of Indian red jungle fowl semen and is a better replacement of glycerol. © 2017 2023-02-20T10:28:34Z 2023-02-20T10:28:34Z 2017 journal article Theriogenology 103: 83-89 (2017) 0093-691X http://hdl.handle.net/20.500.12792/3615 http://hdl.handle.net/10261/293445 10.1016/j.theriogenology.2017.07.018 1879-3231 en none Elsevier |
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Cryo-damage Motility Fertility Acrosome Sperm viability Indian red jungle fowl Cryo-damage Motility Fertility Acrosome Sperm viability Indian red jungle fowl |
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Cryo-damage Motility Fertility Acrosome Sperm viability Indian red jungle fowl Cryo-damage Motility Fertility Acrosome Sperm viability Indian red jungle fowl Rakha, B. A. Ansari, M. S. Akhter, S. Zafar, Z. Naseer, A. Hussain, I. Santiago Moreno, Julián Blesbois, E. Dimethyleacetamide improves the cryosurvivability of Indian red jungle fowl (Gallus gallus murghi) sperm |
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It was hypothesized that dimethyleacetamide (DMA) can be used as an alternate to glycerol for cryopreservation of Indian red jungle fowl semen. Four concentrations of DMA (4%, 6%, 8% and 10%) in extender were compared with previously optimized cryopreservation protocol based on 20% glycerol (control) for Indian red jungle fowl. Sperm motility, plasma membrane integrity, viability, and acrosome integrity were assessed at the stage of post-dilution, cooling, equilibration, and freeze-thawing. The whole experiment was repeated/replicated for five times independently. Sperm motility, plasma membrane integrity, viability and acrosome integrity were recorded highest (P < 0.05) at post-dilution, cooling, equilibration, and freeze-thawing in extender having 6% DMA compared to control and other experimental extenders. The highest (P < 0.05) recovery rates of all aforementioned parameters were also recorded in extender having 6% DMA; thus, 6% DMA was further compared with control (20% glycerol) for fertility after artificial insemination. Eggs were collected for five days after artificial insemination with semen cryopreserved in extender containing 6% DMA and control. The higher no. of fertilized eggs, fertility, no. of hatched eggs, hatch (%) and hatchability were recorded with semen cryopreserved in extender having 6% DMA compared to control. It is concluded that 6% DMA maintained higher post-thaw quality and fertility of Indian red jungle fowl semen and is a better replacement of glycerol. © 2017 |
format |
journal article |
topic_facet |
Cryo-damage Motility Fertility Acrosome Sperm viability Indian red jungle fowl |
author |
Rakha, B. A. Ansari, M. S. Akhter, S. Zafar, Z. Naseer, A. Hussain, I. Santiago Moreno, Julián Blesbois, E. |
author_facet |
Rakha, B. A. Ansari, M. S. Akhter, S. Zafar, Z. Naseer, A. Hussain, I. Santiago Moreno, Julián Blesbois, E. |
author_sort |
Rakha, B. A. |
title |
Dimethyleacetamide improves the cryosurvivability of Indian red jungle fowl (Gallus gallus murghi) sperm |
title_short |
Dimethyleacetamide improves the cryosurvivability of Indian red jungle fowl (Gallus gallus murghi) sperm |
title_full |
Dimethyleacetamide improves the cryosurvivability of Indian red jungle fowl (Gallus gallus murghi) sperm |
title_fullStr |
Dimethyleacetamide improves the cryosurvivability of Indian red jungle fowl (Gallus gallus murghi) sperm |
title_full_unstemmed |
Dimethyleacetamide improves the cryosurvivability of Indian red jungle fowl (Gallus gallus murghi) sperm |
title_sort |
dimethyleacetamide improves the cryosurvivability of indian red jungle fowl (gallus gallus murghi) sperm |
publisher |
Elsevier |
publishDate |
2017 |
url |
http://hdl.handle.net/20.500.12792/3615 http://hdl.handle.net/10261/293445 |
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