Protein production by Escherichia coli wild-type and DeltaptsG mutant strains with IPTG induction at the onset
During Escherichia coli growth on glucose, uptake exceeds the requirement of flux to precursors and the surplus is excreted as acetate. Beside the loss of carbon source, the excretion of a weak acid may result in increased energetic demands and hence a decreased yield. The deletion of ptsG, the gene coding for one of the components (IICB(Glc)) of the glucose-phosphoenolpyruvate phosphotransferase system (Glc-PTS) reduced glucose consumption and acetate excretion. Induction of protein production at the onset of cultivation decreased growth rate and glucose consumption rate for both the WT and the mutant strains. The mutant strain produced beta-galactosidase at higher rates than the wild-type strain while directing more carbon into biomass and CO(2) and less into acetate.
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Format: | journal article biblioteca |
Language: | English |
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Oxford University Press
2008
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Subjects: | E. coli, ptsG deletion, Protein production, Batch-cultivation, Induction, Carbon-Xux, Acetic acid formation, |
Online Access: | http://hdl.handle.net/20.500.12792/3503 http://hdl.handle.net/10261/293372 |
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dig-inia-es-10261-2933722023-02-20T10:27:52Z Protein production by Escherichia coli wild-type and DeltaptsG mutant strains with IPTG induction at the onset Picón Gálvez, Antonia María de Mattos, M. J. Postma, P. W. E. coli ptsG deletion Protein production Batch-cultivation Induction Carbon-Xux Acetic acid formation During Escherichia coli growth on glucose, uptake exceeds the requirement of flux to precursors and the surplus is excreted as acetate. Beside the loss of carbon source, the excretion of a weak acid may result in increased energetic demands and hence a decreased yield. The deletion of ptsG, the gene coding for one of the components (IICB(Glc)) of the glucose-phosphoenolpyruvate phosphotransferase system (Glc-PTS) reduced glucose consumption and acetate excretion. Induction of protein production at the onset of cultivation decreased growth rate and glucose consumption rate for both the WT and the mutant strains. The mutant strain produced beta-galactosidase at higher rates than the wild-type strain while directing more carbon into biomass and CO(2) and less into acetate. 2023-02-20T10:27:52Z 2023-02-20T10:27:52Z 2008 journal article Journal of Industrial Microbiology and Biotechnology 35(4): 213-218 (2008) 1367-5435 http://hdl.handle.net/20.500.12792/3503 http://hdl.handle.net/10261/293372 10.1007/s10295-007-0285-6 1476-5535 en none Oxford University Press |
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E. coli ptsG deletion Protein production Batch-cultivation Induction Carbon-Xux Acetic acid formation E. coli ptsG deletion Protein production Batch-cultivation Induction Carbon-Xux Acetic acid formation |
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E. coli ptsG deletion Protein production Batch-cultivation Induction Carbon-Xux Acetic acid formation E. coli ptsG deletion Protein production Batch-cultivation Induction Carbon-Xux Acetic acid formation Picón Gálvez, Antonia María de Mattos, M. J. Postma, P. W. Protein production by Escherichia coli wild-type and DeltaptsG mutant strains with IPTG induction at the onset |
description |
During Escherichia coli growth on glucose, uptake exceeds the requirement of flux to precursors and the surplus is excreted as acetate. Beside the loss of carbon source, the excretion of a weak acid may result in increased energetic demands and hence a decreased yield. The deletion of ptsG, the gene coding for one of the components (IICB(Glc)) of the glucose-phosphoenolpyruvate phosphotransferase system (Glc-PTS) reduced glucose consumption and acetate excretion. Induction of protein production at the onset of cultivation decreased growth rate and glucose consumption rate for both the WT and the mutant strains. The mutant strain produced beta-galactosidase at higher rates than the wild-type strain while directing more carbon into biomass and CO(2) and less into acetate. |
format |
journal article |
topic_facet |
E. coli ptsG deletion Protein production Batch-cultivation Induction Carbon-Xux Acetic acid formation |
author |
Picón Gálvez, Antonia María de Mattos, M. J. Postma, P. W. |
author_facet |
Picón Gálvez, Antonia María de Mattos, M. J. Postma, P. W. |
author_sort |
Picón Gálvez, Antonia María |
title |
Protein production by Escherichia coli wild-type and DeltaptsG mutant strains with IPTG induction at the onset |
title_short |
Protein production by Escherichia coli wild-type and DeltaptsG mutant strains with IPTG induction at the onset |
title_full |
Protein production by Escherichia coli wild-type and DeltaptsG mutant strains with IPTG induction at the onset |
title_fullStr |
Protein production by Escherichia coli wild-type and DeltaptsG mutant strains with IPTG induction at the onset |
title_full_unstemmed |
Protein production by Escherichia coli wild-type and DeltaptsG mutant strains with IPTG induction at the onset |
title_sort |
protein production by escherichia coli wild-type and deltaptsg mutant strains with iptg induction at the onset |
publisher |
Oxford University Press |
publishDate |
2008 |
url |
http://hdl.handle.net/20.500.12792/3503 http://hdl.handle.net/10261/293372 |
work_keys_str_mv |
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_version_ |
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