Effect of bovine oviductal fluid on development and quality of bovine embryos produced in vitro
To evaluate the effect of bovine oviductal fluid (OF) supplementation during in vitro culture of bovine embryos on their development and quality, in vitro-produced zygotes were cultured in synthetic oviductal fluid (SOF; negative control; C-) supplemented with OF or 5% fetal calf serum (positive control; C+). Embryo development was recorded on Days 7-9 after insemination and blastocyst quality was assessed through cryotolerance, differential cell counting of the inner cell mass and trophectoderm, and gene expression. OF was added to the culture medium at concentrations ranging from 0.625% to 25%. The higher OF concentrations (5%, 10% and 25%) had a detrimental effect on embryo development. Lower OF concentrations (1.25% and 0.625%) supported embryo development until Day 9 (27.5%) and produced higher-quality blastocysts, as reflected by their cryotolerance (53.6% and 57.7% survival at 72h, respectively, vs 25.9% in C+) and total cell number (mean (± s.e.m.) 165.1±4.7 and 156.2±4.2, respectively, vs 127.7±4.9 in C- and 143.1±4.9 in C+). Consistent with these data, upregulation of the water channel aquaporin 3 (AQP3) mRNA was observed in blastocysts supplemented with 1.25% OF compared with C- and C+. Serum supplementation resulted in a reduction in the expression of glucose and lipid metabolism-related genes and downregulation of the epigenetic-related genes DNA methyltransferase 3A (DNMT3A) and insulin-like growth factor 2 receptor (IGF2R). In conclusion, in vitro culture with low concentrations of OF has a positive effect on the development and quality of bovine embryos.
Main Authors: | , , , , , , , |
---|---|
Format: | journal article biblioteca |
Language: | English |
Published: |
CSIRO Publishing
2017
|
Subjects: | Embryo culture, Gene expression, |
Online Access: | http://hdl.handle.net/20.500.12792/2420 http://hdl.handle.net/10261/293194 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-inia-es-10261-293194 |
---|---|
record_format |
koha |
spelling |
dig-inia-es-10261-2931942023-02-20T10:25:58Z Effect of bovine oviductal fluid on development and quality of bovine embryos produced in vitro Lopera-Vasquez, R. Hamdi, M. Maillo, V. Lloreda, V. Coy, P. Gutiérrez Adán, Alfonso Bermejo Álvarez, Pablo Rizos Dimitrios, Dimitrios Embryo culture Gene expression To evaluate the effect of bovine oviductal fluid (OF) supplementation during in vitro culture of bovine embryos on their development and quality, in vitro-produced zygotes were cultured in synthetic oviductal fluid (SOF; negative control; C-) supplemented with OF or 5% fetal calf serum (positive control; C+). Embryo development was recorded on Days 7-9 after insemination and blastocyst quality was assessed through cryotolerance, differential cell counting of the inner cell mass and trophectoderm, and gene expression. OF was added to the culture medium at concentrations ranging from 0.625% to 25%. The higher OF concentrations (5%, 10% and 25%) had a detrimental effect on embryo development. Lower OF concentrations (1.25% and 0.625%) supported embryo development until Day 9 (27.5%) and produced higher-quality blastocysts, as reflected by their cryotolerance (53.6% and 57.7% survival at 72h, respectively, vs 25.9% in C+) and total cell number (mean (± s.e.m.) 165.1±4.7 and 156.2±4.2, respectively, vs 127.7±4.9 in C- and 143.1±4.9 in C+). Consistent with these data, upregulation of the water channel aquaporin 3 (AQP3) mRNA was observed in blastocysts supplemented with 1.25% OF compared with C- and C+. Serum supplementation resulted in a reduction in the expression of glucose and lipid metabolism-related genes and downregulation of the epigenetic-related genes DNA methyltransferase 3A (DNMT3A) and insulin-like growth factor 2 receptor (IGF2R). In conclusion, in vitro culture with low concentrations of OF has a positive effect on the development and quality of bovine embryos. 2023-02-20T10:25:58Z 2023-02-20T10:25:58Z 2017 journal article Reproduction, Fertility and Development 29(3): 621-629 (2017)Reproduction, Fertility and Development 29(3): 621-629 (2017)Reproduction, Fertility and Development 29(3): 621-629 (2017) 1031-3613 http://hdl.handle.net/20.500.12792/2420 http://hdl.handle.net/10261/293194 10.1071/RD15238 1448-5990 en none CSIRO Publishing |
institution |
INIA ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-inia-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del INIA España |
language |
English |
topic |
Embryo culture Gene expression Embryo culture Gene expression |
spellingShingle |
Embryo culture Gene expression Embryo culture Gene expression Lopera-Vasquez, R. Hamdi, M. Maillo, V. Lloreda, V. Coy, P. Gutiérrez Adán, Alfonso Bermejo Álvarez, Pablo Rizos Dimitrios, Dimitrios Effect of bovine oviductal fluid on development and quality of bovine embryos produced in vitro |
description |
To evaluate the effect of bovine oviductal fluid (OF) supplementation during in vitro culture of bovine embryos on their development and quality, in vitro-produced zygotes were cultured in synthetic oviductal fluid (SOF; negative control; C-) supplemented with OF or 5% fetal calf serum (positive control; C+). Embryo development was recorded on Days 7-9 after insemination and blastocyst quality was assessed through cryotolerance, differential cell counting of the inner cell mass and trophectoderm, and gene expression. OF was added to the culture medium at concentrations ranging from 0.625% to 25%. The higher OF concentrations (5%, 10% and 25%) had a detrimental effect on embryo development. Lower OF concentrations (1.25% and 0.625%) supported embryo development until Day 9 (27.5%) and produced higher-quality blastocysts, as reflected by their cryotolerance (53.6% and 57.7% survival at 72h, respectively, vs 25.9% in C+) and total cell number (mean (± s.e.m.) 165.1±4.7 and 156.2±4.2, respectively, vs 127.7±4.9 in C- and 143.1±4.9 in C+). Consistent with these data, upregulation of the water channel aquaporin 3 (AQP3) mRNA was observed in blastocysts supplemented with 1.25% OF compared with C- and C+. Serum supplementation resulted in a reduction in the expression of glucose and lipid metabolism-related genes and downregulation of the epigenetic-related genes DNA methyltransferase 3A (DNMT3A) and insulin-like growth factor 2 receptor (IGF2R). In conclusion, in vitro culture with low concentrations of OF has a positive effect on the development and quality of bovine embryos. |
format |
journal article |
topic_facet |
Embryo culture Gene expression |
author |
Lopera-Vasquez, R. Hamdi, M. Maillo, V. Lloreda, V. Coy, P. Gutiérrez Adán, Alfonso Bermejo Álvarez, Pablo Rizos Dimitrios, Dimitrios |
author_facet |
Lopera-Vasquez, R. Hamdi, M. Maillo, V. Lloreda, V. Coy, P. Gutiérrez Adán, Alfonso Bermejo Álvarez, Pablo Rizos Dimitrios, Dimitrios |
author_sort |
Lopera-Vasquez, R. |
title |
Effect of bovine oviductal fluid on development and quality of bovine embryos produced in vitro |
title_short |
Effect of bovine oviductal fluid on development and quality of bovine embryos produced in vitro |
title_full |
Effect of bovine oviductal fluid on development and quality of bovine embryos produced in vitro |
title_fullStr |
Effect of bovine oviductal fluid on development and quality of bovine embryos produced in vitro |
title_full_unstemmed |
Effect of bovine oviductal fluid on development and quality of bovine embryos produced in vitro |
title_sort |
effect of bovine oviductal fluid on development and quality of bovine embryos produced in vitro |
publisher |
CSIRO Publishing |
publishDate |
2017 |
url |
http://hdl.handle.net/20.500.12792/2420 http://hdl.handle.net/10261/293194 |
work_keys_str_mv |
AT loperavasquezr effectofbovineoviductalfluidondevelopmentandqualityofbovineembryosproducedinvitro AT hamdim effectofbovineoviductalfluidondevelopmentandqualityofbovineembryosproducedinvitro AT maillov effectofbovineoviductalfluidondevelopmentandqualityofbovineembryosproducedinvitro AT lloredav effectofbovineoviductalfluidondevelopmentandqualityofbovineembryosproducedinvitro AT coyp effectofbovineoviductalfluidondevelopmentandqualityofbovineembryosproducedinvitro AT gutierrezadanalfonso effectofbovineoviductalfluidondevelopmentandqualityofbovineembryosproducedinvitro AT bermejoalvarezpablo effectofbovineoviductalfluidondevelopmentandqualityofbovineembryosproducedinvitro AT rizosdimitriosdimitrios effectofbovineoviductalfluidondevelopmentandqualityofbovineembryosproducedinvitro |
_version_ |
1767603442084216832 |