Genetic assessment of African swine fever isolates involved in outbreaks in the Democratic Republic Of Congo between 2005 and 2012 reveals co-circulation of p72 genotypes I, IX and XIV, including 19 variants
African swine fever (ASF) is a devastating disease of domestic pigs. It is a socioeconomically important disease, initially described from Kenya, but subsequently reported in most Sub-Saharan countries. ASF spread to Europe, South America and the Caribbean through multiple introductions which were initially eradicated—except for Sardinia—followed by re-introduction into Europe in 2007. In this study of ASF within the Democratic Republic of the Congo, 62 domestic pig samples, collected between 2005-2012, were examined for viral DNA and sequencing at multiple loci C-terminus of the B646L gene (p72 protein), central hypervariable region (CVR) of the B602L gene, and the E183L gene (p54 protein). Phylogenetic analyses identified three circulating genotypes I (64.5% of samples), IX (32.3%), and XIV (3.2%). This is the first evidence of genotypes IX and XIV within this country. Examination of the CVR revealed high levels of intra-genotypic variation, with 19 identified variants. © 2017 by the authors; licensee MDPI, Basel, Switzerland.
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Format: | artículo biblioteca |
Language: | English |
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Multidisciplinary Digital Publishing Institute
2017
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Subjects: | African swine fever virus, Outbreaks, Democratic Republic of Congo, Swine, Genotypes, Molecular epidemiology, p72 gene, p54 gene, CVR, |
Online Access: | http://hdl.handle.net/20.500.12792/3044 http://hdl.handle.net/10261/293077 |
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dig-inia-es-10261-2930772023-02-20T07:35:34Z Genetic assessment of African swine fever isolates involved in outbreaks in the Democratic Republic Of Congo between 2005 and 2012 reveals co-circulation of p72 genotypes I, IX and XIV, including 19 variants Mulumba-Mfumu, L. K. Achenbach, J. E. Mauldin, M. R. Dixon, L. K. Tshilenge, C. G. Thiry, E. Moreno, N. Blanco Lavilla, Esther Saegerman, C. Lamien, C. E. Diallo, A. African swine fever virus Outbreaks Democratic Republic of Congo Swine Genotypes Molecular epidemiology p72 gene p54 gene CVR African swine fever (ASF) is a devastating disease of domestic pigs. It is a socioeconomically important disease, initially described from Kenya, but subsequently reported in most Sub-Saharan countries. ASF spread to Europe, South America and the Caribbean through multiple introductions which were initially eradicated—except for Sardinia—followed by re-introduction into Europe in 2007. In this study of ASF within the Democratic Republic of the Congo, 62 domestic pig samples, collected between 2005-2012, were examined for viral DNA and sequencing at multiple loci C-terminus of the B646L gene (p72 protein), central hypervariable region (CVR) of the B602L gene, and the E183L gene (p54 protein). Phylogenetic analyses identified three circulating genotypes I (64.5% of samples), IX (32.3%), and XIV (3.2%). This is the first evidence of genotypes IX and XIV within this country. Examination of the CVR revealed high levels of intra-genotypic variation, with 19 identified variants. © 2017 by the authors; licensee MDPI, Basel, Switzerland. 2023-02-20T07:35:34Z 2023-02-20T07:35:34Z 2017 artículo Viruses 9(2): e31 (2017) http://hdl.handle.net/20.500.12792/3044 http://hdl.handle.net/10261/293077 10.3390/v9020031 1999-4915 en open Multidisciplinary Digital Publishing Institute |
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African swine fever virus Outbreaks Democratic Republic of Congo Swine Genotypes Molecular epidemiology p72 gene p54 gene CVR African swine fever virus Outbreaks Democratic Republic of Congo Swine Genotypes Molecular epidemiology p72 gene p54 gene CVR |
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African swine fever virus Outbreaks Democratic Republic of Congo Swine Genotypes Molecular epidemiology p72 gene p54 gene CVR African swine fever virus Outbreaks Democratic Republic of Congo Swine Genotypes Molecular epidemiology p72 gene p54 gene CVR Mulumba-Mfumu, L. K. Achenbach, J. E. Mauldin, M. R. Dixon, L. K. Tshilenge, C. G. Thiry, E. Moreno, N. Blanco Lavilla, Esther Saegerman, C. Lamien, C. E. Diallo, A. Genetic assessment of African swine fever isolates involved in outbreaks in the Democratic Republic Of Congo between 2005 and 2012 reveals co-circulation of p72 genotypes I, IX and XIV, including 19 variants |
description |
African swine fever (ASF) is a devastating disease of domestic pigs. It is a socioeconomically important disease, initially described from Kenya, but subsequently reported in most Sub-Saharan countries. ASF spread to Europe, South America and the Caribbean through multiple introductions which were initially eradicated—except for Sardinia—followed by re-introduction into Europe in 2007. In this study of ASF within the Democratic Republic of the Congo, 62 domestic pig samples, collected between 2005-2012, were examined for viral DNA and sequencing at multiple loci C-terminus of the B646L gene (p72 protein), central hypervariable region (CVR) of the B602L gene, and the E183L gene (p54 protein). Phylogenetic analyses identified three circulating genotypes I (64.5% of samples), IX (32.3%), and XIV (3.2%). This is the first evidence of genotypes IX and XIV within this country. Examination of the CVR revealed high levels of intra-genotypic variation, with 19 identified variants. © 2017 by the authors; licensee MDPI, Basel, Switzerland. |
format |
artículo |
topic_facet |
African swine fever virus Outbreaks Democratic Republic of Congo Swine Genotypes Molecular epidemiology p72 gene p54 gene CVR |
author |
Mulumba-Mfumu, L. K. Achenbach, J. E. Mauldin, M. R. Dixon, L. K. Tshilenge, C. G. Thiry, E. Moreno, N. Blanco Lavilla, Esther Saegerman, C. Lamien, C. E. Diallo, A. |
author_facet |
Mulumba-Mfumu, L. K. Achenbach, J. E. Mauldin, M. R. Dixon, L. K. Tshilenge, C. G. Thiry, E. Moreno, N. Blanco Lavilla, Esther Saegerman, C. Lamien, C. E. Diallo, A. |
author_sort |
Mulumba-Mfumu, L. K. |
title |
Genetic assessment of African swine fever isolates involved in outbreaks in the Democratic Republic Of Congo between 2005 and 2012 reveals co-circulation of p72 genotypes I, IX and XIV, including 19 variants |
title_short |
Genetic assessment of African swine fever isolates involved in outbreaks in the Democratic Republic Of Congo between 2005 and 2012 reveals co-circulation of p72 genotypes I, IX and XIV, including 19 variants |
title_full |
Genetic assessment of African swine fever isolates involved in outbreaks in the Democratic Republic Of Congo between 2005 and 2012 reveals co-circulation of p72 genotypes I, IX and XIV, including 19 variants |
title_fullStr |
Genetic assessment of African swine fever isolates involved in outbreaks in the Democratic Republic Of Congo between 2005 and 2012 reveals co-circulation of p72 genotypes I, IX and XIV, including 19 variants |
title_full_unstemmed |
Genetic assessment of African swine fever isolates involved in outbreaks in the Democratic Republic Of Congo between 2005 and 2012 reveals co-circulation of p72 genotypes I, IX and XIV, including 19 variants |
title_sort |
genetic assessment of african swine fever isolates involved in outbreaks in the democratic republic of congo between 2005 and 2012 reveals co-circulation of p72 genotypes i, ix and xiv, including 19 variants |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2017 |
url |
http://hdl.handle.net/20.500.12792/3044 http://hdl.handle.net/10261/293077 |
work_keys_str_mv |
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