Exocannabinoids effect on in vitro bovine oocyte maturation via activation of AKT and ERK1/2
Endocannabinoids are known to mediate practically all reproductive events in mammals; however, little is known about their role in oocyte maturation. Through RT-PCR and immunocytochemistry, this study confirms the presence of CB1 and CB2 cannabinoid receptors in bovine oocytes and shows how exposure to the exogenous cannabinoids HU-210 and THC during their in vitro maturation (IVM) activates the phosphorylation of AKT and ERK1/2 proteins associated with the resumption of meiosis. Although supplementation with HU-210 or THC during IVM did not increase blastocyst yields, the expression of interferon tau (IFNτ) and gap junction alpha-1 protein (GJA1) was enhanced at the blastocyst stage. Our data suggest that cannabinoid agonists may be useful IVM supplements as their presence during oocyte maturation upregulates the expression in blastocysts of key genes for embryo quality. © 2016 Society for Reproduction and Fertility.
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BioScientifica
2016
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Online Access: | http://hdl.handle.net/20.500.12792/2458 http://hdl.handle.net/10261/293074 |
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dig-inia-es-10261-2930742023-02-20T07:35:31Z Exocannabinoids effect on in vitro bovine oocyte maturation via activation of AKT and ERK1/2 López-Cardona, A. P. Sánchez-Calabuig, M. J. Beltrán Breña, Paula Agirregoitia, N. Rizos Dimitrios, Dimitrios Agirregoitia, E. Gutiérrez Adán, Alfonso Endocannabinoids are known to mediate practically all reproductive events in mammals; however, little is known about their role in oocyte maturation. Through RT-PCR and immunocytochemistry, this study confirms the presence of CB1 and CB2 cannabinoid receptors in bovine oocytes and shows how exposure to the exogenous cannabinoids HU-210 and THC during their in vitro maturation (IVM) activates the phosphorylation of AKT and ERK1/2 proteins associated with the resumption of meiosis. Although supplementation with HU-210 or THC during IVM did not increase blastocyst yields, the expression of interferon tau (IFNτ) and gap junction alpha-1 protein (GJA1) was enhanced at the blastocyst stage. Our data suggest that cannabinoid agonists may be useful IVM supplements as their presence during oocyte maturation upregulates the expression in blastocysts of key genes for embryo quality. © 2016 Society for Reproduction and Fertility. 2023-02-20T07:35:31Z 2023-02-20T07:35:31Z 2016 artículo Reproduction 152: 1-10 (2016) 1470-1626 http://hdl.handle.net/20.500.12792/2458 http://hdl.handle.net/10261/293074 10.1530/REP-16-0199 1741-7899 en none BioScientifica |
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Endocannabinoids are known to mediate practically all reproductive events in mammals; however, little is known about their role in oocyte maturation. Through RT-PCR and immunocytochemistry, this study confirms the presence of CB1 and CB2 cannabinoid receptors in bovine oocytes and shows how exposure to the exogenous cannabinoids HU-210 and THC during their in vitro maturation (IVM) activates the phosphorylation of AKT and ERK1/2 proteins associated with the resumption of meiosis. Although supplementation with HU-210 or THC during IVM did not increase blastocyst yields, the expression of interferon tau (IFNτ) and gap junction alpha-1 protein (GJA1) was enhanced at the blastocyst stage. Our data suggest that cannabinoid agonists may be useful IVM supplements as their presence during oocyte maturation upregulates the expression in blastocysts of key genes for embryo quality. © 2016 Society for Reproduction and Fertility. |
format |
artículo |
author |
López-Cardona, A. P. Sánchez-Calabuig, M. J. Beltrán Breña, Paula Agirregoitia, N. Rizos Dimitrios, Dimitrios Agirregoitia, E. Gutiérrez Adán, Alfonso |
spellingShingle |
López-Cardona, A. P. Sánchez-Calabuig, M. J. Beltrán Breña, Paula Agirregoitia, N. Rizos Dimitrios, Dimitrios Agirregoitia, E. Gutiérrez Adán, Alfonso Exocannabinoids effect on in vitro bovine oocyte maturation via activation of AKT and ERK1/2 |
author_facet |
López-Cardona, A. P. Sánchez-Calabuig, M. J. Beltrán Breña, Paula Agirregoitia, N. Rizos Dimitrios, Dimitrios Agirregoitia, E. Gutiérrez Adán, Alfonso |
author_sort |
López-Cardona, A. P. |
title |
Exocannabinoids effect on in vitro bovine oocyte maturation via activation of AKT and ERK1/2 |
title_short |
Exocannabinoids effect on in vitro bovine oocyte maturation via activation of AKT and ERK1/2 |
title_full |
Exocannabinoids effect on in vitro bovine oocyte maturation via activation of AKT and ERK1/2 |
title_fullStr |
Exocannabinoids effect on in vitro bovine oocyte maturation via activation of AKT and ERK1/2 |
title_full_unstemmed |
Exocannabinoids effect on in vitro bovine oocyte maturation via activation of AKT and ERK1/2 |
title_sort |
exocannabinoids effect on in vitro bovine oocyte maturation via activation of akt and erk1/2 |
publisher |
BioScientifica |
publishDate |
2016 |
url |
http://hdl.handle.net/20.500.12792/2458 http://hdl.handle.net/10261/293074 |
work_keys_str_mv |
AT lopezcardonaap exocannabinoidseffectoninvitrobovineoocytematurationviaactivationofaktanderk12 AT sanchezcalabuigmj exocannabinoidseffectoninvitrobovineoocytematurationviaactivationofaktanderk12 AT beltranbrenapaula exocannabinoidseffectoninvitrobovineoocytematurationviaactivationofaktanderk12 AT agirregoitian exocannabinoidseffectoninvitrobovineoocytematurationviaactivationofaktanderk12 AT rizosdimitriosdimitrios exocannabinoidseffectoninvitrobovineoocytematurationviaactivationofaktanderk12 AT agirregoitiae exocannabinoidseffectoninvitrobovineoocytematurationviaactivationofaktanderk12 AT gutierrezadanalfonso exocannabinoidseffectoninvitrobovineoocytematurationviaactivationofaktanderk12 |
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