Genetic and molecular analyses of natural variation indicate CBF2 as a candidate gene for underlying a freezing tolerance quantitative trait locus in Arabidopsis

Genetic and molecular analyses of natural variation indicate CBF2 as a candidate gene for underlying a freezing tolerance quantitative trait locus in Arabidopsis

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Natural variation for freezing tolerance is a major component of adaptation and geographic distribution of plant species. However, little is known about the genes and molecular mechanisms that determine its naturally occurring diversity. We have analyzed the intraspecific freezing tolerance variation existent between two geographically distant accessions of Arabidopsis (Arabidopsis thaliana), Cape Verde Islands (Cvi) and Landsberg erecta (Ler). They differed in their freezing tolerance before and after cold acclimation, as well as in the cold acclimation response in relation to photoperiod conditions. Using a quantitative genetic approach, we found that freezing tolerance differences after cold acclimation were determined by seven quantitative trait loci (QTL), named FREEZING TOLERANCE QTL 1 (FTQ1) to FTQ7. FTQ4 was the QTL with the largest effect detected in two photoperiod conditions, while five other FTQ loci behaved as photoperiod dependent. FTQ4 colocated with the tandem repeated genes C-REPEAT BINDING FACTOR 1 (CBF1), CBF2, and CBF3, which encode transcriptional activators involved in the cold acclimation response. The low freezing tolerance of FTQ4-Cvi alleles was associated with a deletion of the promoter region of Cvi CBF2, and with low RNA expression of CBF2 and of several CBF target genes. Genetic complementation of FTQ4-Cvi plants with a CBF2-Ler transgene suggests that such CBF2 allelic variation is the cause of CBF2 misexpression and the molecular basis of FTQ4.

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Main Authors: Alonso-Blanco, C., Gomez-Mena, C., Llorente De Gracia, Francisco, Koornneef, M., Salinas, J., Martínez-Zapater, J. M.
Format: artículo biblioteca
Language:English
Published: Oxford University Press 2005
Online Access:http://hdl.handle.net/20.500.12792/6004
http://hdl.handle.net/10261/293009
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spelling dig-inia-es-10261-2930092023-02-20T07:34:48Z Genetic and molecular analyses of natural variation indicate CBF2 as a candidate gene for underlying a freezing tolerance quantitative trait locus in Arabidopsis Alonso-Blanco, C. Gomez-Mena, C. Llorente De Gracia, Francisco Koornneef, M. Salinas, J. Martínez-Zapater, J. M. Natural variation for freezing tolerance is a major component of adaptation and geographic distribution of plant species. However, little is known about the genes and molecular mechanisms that determine its naturally occurring diversity. We have analyzed the intraspecific freezing tolerance variation existent between two geographically distant accessions of Arabidopsis (Arabidopsis thaliana), Cape Verde Islands (Cvi) and Landsberg erecta (Ler). They differed in their freezing tolerance before and after cold acclimation, as well as in the cold acclimation response in relation to photoperiod conditions. Using a quantitative genetic approach, we found that freezing tolerance differences after cold acclimation were determined by seven quantitative trait loci (QTL), named FREEZING TOLERANCE QTL 1 (FTQ1) to FTQ7. FTQ4 was the QTL with the largest effect detected in two photoperiod conditions, while five other FTQ loci behaved as photoperiod dependent. FTQ4 colocated with the tandem repeated genes C-REPEAT BINDING FACTOR 1 (CBF1), CBF2, and CBF3, which encode transcriptional activators involved in the cold acclimation response. The low freezing tolerance of FTQ4-Cvi alleles was associated with a deletion of the promoter region of Cvi CBF2, and with low RNA expression of CBF2 and of several CBF target genes. Genetic complementation of FTQ4-Cvi plants with a CBF2-Ler transgene suggests that such CBF2 allelic variation is the cause of CBF2 misexpression and the molecular basis of FTQ4. 2023-02-20T07:34:47Z 2023-02-20T07:34:47Z 2005 artículo Plant Physiology 139(3): 1304-1312 (2005) 0032-0889 http://hdl.handle.net/20.500.12792/6004 http://hdl.handle.net/10261/293009 10.1104/pp.105.068510 1532-2548 en none Oxford University Press
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collection DSpace
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databasecode dig-inia-es
tag biblioteca
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language English
description Natural variation for freezing tolerance is a major component of adaptation and geographic distribution of plant species. However, little is known about the genes and molecular mechanisms that determine its naturally occurring diversity. We have analyzed the intraspecific freezing tolerance variation existent between two geographically distant accessions of Arabidopsis (Arabidopsis thaliana), Cape Verde Islands (Cvi) and Landsberg erecta (Ler). They differed in their freezing tolerance before and after cold acclimation, as well as in the cold acclimation response in relation to photoperiod conditions. Using a quantitative genetic approach, we found that freezing tolerance differences after cold acclimation were determined by seven quantitative trait loci (QTL), named FREEZING TOLERANCE QTL 1 (FTQ1) to FTQ7. FTQ4 was the QTL with the largest effect detected in two photoperiod conditions, while five other FTQ loci behaved as photoperiod dependent. FTQ4 colocated with the tandem repeated genes C-REPEAT BINDING FACTOR 1 (CBF1), CBF2, and CBF3, which encode transcriptional activators involved in the cold acclimation response. The low freezing tolerance of FTQ4-Cvi alleles was associated with a deletion of the promoter region of Cvi CBF2, and with low RNA expression of CBF2 and of several CBF target genes. Genetic complementation of FTQ4-Cvi plants with a CBF2-Ler transgene suggests that such CBF2 allelic variation is the cause of CBF2 misexpression and the molecular basis of FTQ4.
format artículo
author Alonso-Blanco, C.
Gomez-Mena, C.
Llorente De Gracia, Francisco
Koornneef, M.
Salinas, J.
Martínez-Zapater, J. M.
spellingShingle Alonso-Blanco, C.
Gomez-Mena, C.
Llorente De Gracia, Francisco
Koornneef, M.
Salinas, J.
Martínez-Zapater, J. M.
Genetic and molecular analyses of natural variation indicate CBF2 as a candidate gene for underlying a freezing tolerance quantitative trait locus in Arabidopsis
author_facet Alonso-Blanco, C.
Gomez-Mena, C.
Llorente De Gracia, Francisco
Koornneef, M.
Salinas, J.
Martínez-Zapater, J. M.
author_sort Alonso-Blanco, C.
title Genetic and molecular analyses of natural variation indicate CBF2 as a candidate gene for underlying a freezing tolerance quantitative trait locus in Arabidopsis
title_short Genetic and molecular analyses of natural variation indicate CBF2 as a candidate gene for underlying a freezing tolerance quantitative trait locus in Arabidopsis
title_full Genetic and molecular analyses of natural variation indicate CBF2 as a candidate gene for underlying a freezing tolerance quantitative trait locus in Arabidopsis
title_fullStr Genetic and molecular analyses of natural variation indicate CBF2 as a candidate gene for underlying a freezing tolerance quantitative trait locus in Arabidopsis
title_full_unstemmed Genetic and molecular analyses of natural variation indicate CBF2 as a candidate gene for underlying a freezing tolerance quantitative trait locus in Arabidopsis
title_sort genetic and molecular analyses of natural variation indicate cbf2 as a candidate gene for underlying a freezing tolerance quantitative trait locus in arabidopsis
publisher Oxford University Press
publishDate 2005
url http://hdl.handle.net/20.500.12792/6004
http://hdl.handle.net/10261/293009
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AT gomezmenac geneticandmolecularanalysesofnaturalvariationindicatecbf2asacandidategeneforunderlyingafreezingtolerancequantitativetraitlocusinarabidopsis
AT llorentedegraciafrancisco geneticandmolecularanalysesofnaturalvariationindicatecbf2asacandidategeneforunderlyingafreezingtolerancequantitativetraitlocusinarabidopsis
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AT martinezzapaterjm geneticandmolecularanalysesofnaturalvariationindicatecbf2asacandidategeneforunderlyingafreezingtolerancequantitativetraitlocusinarabidopsis
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