Protection against West Nile virus infection in mice after inoculation with type I interferon-inducing RNA transcripts
West Nile virus (WNV) is a neurovirulent single stranded RNA mosquito-borne flavivirus, whose main natural hosts are birds, but it also infects humans and horses. Nowadays, no human vaccine is commercially available and clinical treatment is only supportive. Recently, it has been shown that RNA transcripts, mimicking structural domains in the non-coding regions (NCRs) of the foot-and mouth disease virus (FMDV) induce a potent IFN response and antiviral activity in transfected cultured cells, and also reduced mice susceptibility to FMDV. By using different transcripts combinations, administration schedules, and infecting routes and doses, we have demonstrated that these FMDV RNA transcripts protect suckling and adult mice against lethal challenge with WNV. The protective activity induced by the transcripts was systemic and dependent on the infection route and dose. These results confirm the antiviral potential of these synthetic RNAs for fighting viruses of different families relevant for human and animal health. © 2012 Rodríguez-Pulido et al.
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dig-inia-es-10261-2929402023-02-20T07:34:03Z Protection against West Nile virus infection in mice after inoculation with type I interferon-inducing RNA transcripts Rodríguez-Pulido, M. Martín-Acebes, M. A. Escribano Romero, Estela Blázquez Martín, Ana Belén Sobrino, F. Borrego, B. Sáiz, M. Saiz Calahorra, Juan Carlos West Nile virus (WNV) is a neurovirulent single stranded RNA mosquito-borne flavivirus, whose main natural hosts are birds, but it also infects humans and horses. Nowadays, no human vaccine is commercially available and clinical treatment is only supportive. Recently, it has been shown that RNA transcripts, mimicking structural domains in the non-coding regions (NCRs) of the foot-and mouth disease virus (FMDV) induce a potent IFN response and antiviral activity in transfected cultured cells, and also reduced mice susceptibility to FMDV. By using different transcripts combinations, administration schedules, and infecting routes and doses, we have demonstrated that these FMDV RNA transcripts protect suckling and adult mice against lethal challenge with WNV. The protective activity induced by the transcripts was systemic and dependent on the infection route and dose. These results confirm the antiviral potential of these synthetic RNAs for fighting viruses of different families relevant for human and animal health. © 2012 Rodríguez-Pulido et al. 2023-02-20T07:34:03Z 2023-02-20T07:34:03Z 2012 artículo PLoS ONE 7(11): e49494 (2012) http://hdl.handle.net/20.500.12792/3841 http://hdl.handle.net/10261/292940 10.1371/journal.pone.0049494 1932-6203 en open Public Library of Science |
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West Nile virus (WNV) is a neurovirulent single stranded RNA mosquito-borne flavivirus, whose main natural hosts are birds, but it also infects humans and horses. Nowadays, no human vaccine is commercially available and clinical treatment is only supportive. Recently, it has been shown that RNA transcripts, mimicking structural domains in the non-coding regions (NCRs) of the foot-and mouth disease virus (FMDV) induce a potent IFN response and antiviral activity in transfected cultured cells, and also reduced mice susceptibility to FMDV. By using different transcripts combinations, administration schedules, and infecting routes and doses, we have demonstrated that these FMDV RNA transcripts protect suckling and adult mice against lethal challenge with WNV. The protective activity induced by the transcripts was systemic and dependent on the infection route and dose. These results confirm the antiviral potential of these synthetic RNAs for fighting viruses of different families relevant for human and animal health. © 2012 Rodríguez-Pulido et al. |
format |
artículo |
author |
Rodríguez-Pulido, M. Martín-Acebes, M. A. Escribano Romero, Estela Blázquez Martín, Ana Belén Sobrino, F. Borrego, B. Sáiz, M. Saiz Calahorra, Juan Carlos |
spellingShingle |
Rodríguez-Pulido, M. Martín-Acebes, M. A. Escribano Romero, Estela Blázquez Martín, Ana Belén Sobrino, F. Borrego, B. Sáiz, M. Saiz Calahorra, Juan Carlos Protection against West Nile virus infection in mice after inoculation with type I interferon-inducing RNA transcripts |
author_facet |
Rodríguez-Pulido, M. Martín-Acebes, M. A. Escribano Romero, Estela Blázquez Martín, Ana Belén Sobrino, F. Borrego, B. Sáiz, M. Saiz Calahorra, Juan Carlos |
author_sort |
Rodríguez-Pulido, M. |
title |
Protection against West Nile virus infection in mice after inoculation with type I interferon-inducing RNA transcripts |
title_short |
Protection against West Nile virus infection in mice after inoculation with type I interferon-inducing RNA transcripts |
title_full |
Protection against West Nile virus infection in mice after inoculation with type I interferon-inducing RNA transcripts |
title_fullStr |
Protection against West Nile virus infection in mice after inoculation with type I interferon-inducing RNA transcripts |
title_full_unstemmed |
Protection against West Nile virus infection in mice after inoculation with type I interferon-inducing RNA transcripts |
title_sort |
protection against west nile virus infection in mice after inoculation with type i interferon-inducing rna transcripts |
publisher |
Public Library of Science |
publishDate |
2012 |
url |
http://hdl.handle.net/20.500.12792/3841 http://hdl.handle.net/10261/292940 |
work_keys_str_mv |
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