Oocyte developmental failure in response to elevated nonesterified fatty acid concentrations Mechanistic insights
Elevated plasma nonesterified fatty acid (NEFA) concentrations are associated with negative energy balance and metabolic disorders such as obesity and type II diabetes. Such increased plasma NEFA concentrations induce changes in the microenvironment of the ovarian follicle, which can compromise oocyte competence. Exposing oocytes to elevated NEFA concentrations during maturation affects the gene expression and phenotype of the subsequent embryo, notably prompting a disrupted oxidative metabolism. We hypothesized that these changes in the embryo are a consequence of modified energy metabolism in the oocyte. To investigate this, bovine cumulus oocyte complexes were matured under elevated NEFA conditions, and energy metabolism-related gene expression, mitochondrial function, and ultrastructure evaluated. It was found that expression of genes related to REDOX maintenance was modified in NEFA-exposed oocytes, cumulus cells, and resultant blastocysts. Moreover, the expression of genes related to fatty acid synthesis in embryos that developed from NEFA-exposed oocytes was upregulated. From a functional perspective, inhibition of fatty acid b-oxidation in maturing oocytes exposed to elevated NEFA concentrations restored developmental competence. There were no clear differences in mitochondrial morphology or oxygen consumption between treatments, although there was a trend for a higher mitochondrial membrane potential in zygotes derived from NEFA-exposed oocytes. These data show that the degree of mitochondrial fatty acid b-oxidation has a decisive impact on the development of NEFA-exposed oocytes. Furthermore, the gene expression data suggest that the resulting embryos adapt through altered metabolic strategies, which might explain the aberrant energy metabolism previously observed in these embryos originating from NEFA-exposed maturing oocytes. © 2013 Society for Reproduction and Fertility.
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BioScientifica
2013
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dig-inia-es-10261-2928642023-02-20T07:33:13Z Oocyte developmental failure in response to elevated nonesterified fatty acid concentrations Mechanistic insights Van Hoeck, V. Leroy, J. L. M. R. Alvarez, M. A. Rizos Dimitrios, Dimitrios Gutiérrez Adán, Alfonso Schnorbusch, K. Bols, P. E. J. Leese, H. J. Sturmey, R. G. Elevated plasma nonesterified fatty acid (NEFA) concentrations are associated with negative energy balance and metabolic disorders such as obesity and type II diabetes. Such increased plasma NEFA concentrations induce changes in the microenvironment of the ovarian follicle, which can compromise oocyte competence. Exposing oocytes to elevated NEFA concentrations during maturation affects the gene expression and phenotype of the subsequent embryo, notably prompting a disrupted oxidative metabolism. We hypothesized that these changes in the embryo are a consequence of modified energy metabolism in the oocyte. To investigate this, bovine cumulus oocyte complexes were matured under elevated NEFA conditions, and energy metabolism-related gene expression, mitochondrial function, and ultrastructure evaluated. It was found that expression of genes related to REDOX maintenance was modified in NEFA-exposed oocytes, cumulus cells, and resultant blastocysts. Moreover, the expression of genes related to fatty acid synthesis in embryos that developed from NEFA-exposed oocytes was upregulated. From a functional perspective, inhibition of fatty acid b-oxidation in maturing oocytes exposed to elevated NEFA concentrations restored developmental competence. There were no clear differences in mitochondrial morphology or oxygen consumption between treatments, although there was a trend for a higher mitochondrial membrane potential in zygotes derived from NEFA-exposed oocytes. These data show that the degree of mitochondrial fatty acid b-oxidation has a decisive impact on the development of NEFA-exposed oocytes. Furthermore, the gene expression data suggest that the resulting embryos adapt through altered metabolic strategies, which might explain the aberrant energy metabolism previously observed in these embryos originating from NEFA-exposed maturing oocytes. © 2013 Society for Reproduction and Fertility. 2023-02-20T07:33:13Z 2023-02-20T07:33:13Z 2013 artículo Reproduction 145(1): 33-44 (2013) 1470-1626 http://hdl.handle.net/20.500.12792/4620 http://hdl.handle.net/10261/292864 10.1530/REP-12-0174 1741-7899 en none BioScientifica |
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Elevated plasma nonesterified fatty acid (NEFA) concentrations are associated with negative energy balance and metabolic disorders such as obesity and type II diabetes. Such increased plasma NEFA concentrations induce changes in the microenvironment of the ovarian follicle, which can compromise oocyte competence. Exposing oocytes to elevated NEFA concentrations during maturation affects the gene expression and phenotype of the subsequent embryo, notably prompting a disrupted oxidative metabolism. We hypothesized that these changes in the embryo are a consequence of modified energy metabolism in the oocyte. To investigate this, bovine cumulus oocyte complexes were matured under elevated NEFA conditions, and energy metabolism-related gene expression, mitochondrial function, and ultrastructure evaluated. It was found that expression of genes related to REDOX maintenance was modified in NEFA-exposed oocytes, cumulus cells, and resultant blastocysts. Moreover, the expression of genes related to fatty acid synthesis in embryos that developed from NEFA-exposed oocytes was upregulated. From a functional perspective, inhibition of fatty acid b-oxidation in maturing oocytes exposed to elevated NEFA concentrations restored developmental competence. There were no clear differences in mitochondrial morphology or oxygen consumption between treatments, although there was a trend for a higher mitochondrial membrane potential in zygotes derived from NEFA-exposed oocytes. These data show that the degree of mitochondrial fatty acid b-oxidation has a decisive impact on the development of NEFA-exposed oocytes. Furthermore, the gene expression data suggest that the resulting embryos adapt through altered metabolic strategies, which might explain the aberrant energy metabolism previously observed in these embryos originating from NEFA-exposed maturing oocytes. © 2013 Society for Reproduction and Fertility. |
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artículo |
author |
Van Hoeck, V. Leroy, J. L. M. R. Alvarez, M. A. Rizos Dimitrios, Dimitrios Gutiérrez Adán, Alfonso Schnorbusch, K. Bols, P. E. J. Leese, H. J. Sturmey, R. G. |
spellingShingle |
Van Hoeck, V. Leroy, J. L. M. R. Alvarez, M. A. Rizos Dimitrios, Dimitrios Gutiérrez Adán, Alfonso Schnorbusch, K. Bols, P. E. J. Leese, H. J. Sturmey, R. G. Oocyte developmental failure in response to elevated nonesterified fatty acid concentrations Mechanistic insights |
author_facet |
Van Hoeck, V. Leroy, J. L. M. R. Alvarez, M. A. Rizos Dimitrios, Dimitrios Gutiérrez Adán, Alfonso Schnorbusch, K. Bols, P. E. J. Leese, H. J. Sturmey, R. G. |
author_sort |
Van Hoeck, V. |
title |
Oocyte developmental failure in response to elevated nonesterified fatty acid concentrations Mechanistic insights |
title_short |
Oocyte developmental failure in response to elevated nonesterified fatty acid concentrations Mechanistic insights |
title_full |
Oocyte developmental failure in response to elevated nonesterified fatty acid concentrations Mechanistic insights |
title_fullStr |
Oocyte developmental failure in response to elevated nonesterified fatty acid concentrations Mechanistic insights |
title_full_unstemmed |
Oocyte developmental failure in response to elevated nonesterified fatty acid concentrations Mechanistic insights |
title_sort |
oocyte developmental failure in response to elevated nonesterified fatty acid concentrations mechanistic insights |
publisher |
BioScientifica |
publishDate |
2013 |
url |
http://hdl.handle.net/20.500.12792/4620 http://hdl.handle.net/10261/292864 |
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