Molecular differentiation between NS1 gene of a field strain Bluetongue virus serotype 2 (BTV-2) and NS1 gene of an attenuated BTV-2 vaccine

At the end of September 2000, clinical symptoms of Bluetongue appeared in sheep flocks of the Balearic Islands (Spain). The presence of the BTV serotype 2 in tissue and blood samples of affected animals was confirmed by laboratory techniques. A systematic vaccination were carried out in affected areas using a live monovalent serotype 2 vaccine available from Onderstepoort laboratory (South Africa). In order to perform epidemiological studies, a new method to differentiate between the NS1 genes of BTV-2 affecting the Balearic islands and that of the Onderstepoort commercial live virus vaccine (monovalent, serotype 2) has been developed. This procedure is based on the use of an RT-PCR, followed by restriction endonuclease analysis. Epidemiological data of a study carried out in the period January-October 2001 using this procedure are included. © 2002 Elsevier Science B.V. All rights reserved.

Bibliographic Details

Main Authors:	Agüero, M., Arias, M., Romero, L. J., Zamora, M. J., Sánchez-Vizcaíno, J. M.
Format:	artículo biblioteca
Language:	English
Published:	Elsevier 2002
Subjects:	Bluetongue virus, Molecular diagnosis, RT–PCR, Vaccine,
Online Access:	http://hdl.handle.net/20.500.12792/1740 http://hdl.handle.net/10261/292677
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