Comparison of four methods to evaluate sperm DNA integrity between mouse caput and cauda epididymidis
It is well known that transit through the epididymis involves an increase in the compaction of sperm chromatin, which acquires fully condensed status at the caput epididymidis. The purpose of this study was to compare the terminal deoxyribonucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) assay, the comet assay, the sperm chromatin structure assay (SCSA) and the sperm chromatin dispersion (SCD) test by analysing spermatozoa from the caput and cauda epididymidis in order to demonstrate the ability of each technique to discriminate between different degrees of sperm maturity related to chromatin compaction and DNA fragmentation. Our results suggest that some populations of DNA-fragmented spermatozoa associated with immature sperm can only be identified using the comet assay and the SCSA but not with the SCD test or the TUNEL assay. © 2012 AJA, SIMM & SJTU. All rights reserved.
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Format: | artículo biblioteca |
Language: | English |
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Medknow Publications
2012
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Subjects: | DNA damage, Mouse epididymis, Sperm chromatin, Sperm maturation, The comet assay, The sperm chromatin dispersion test, The sperm chromatin structure assay, Tunel, |
Online Access: | http://hdl.handle.net/20.500.12792/3433 http://hdl.handle.net/10261/292620 |
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dig-inia-es-10261-2926202023-02-20T07:30:44Z Comparison of four methods to evaluate sperm DNA integrity between mouse caput and cauda epididymidis Pérez Cerezales, Serafín Miranda, A. Gutiérrez Adán, Alfonso DNA damage Mouse epididymis Sperm chromatin Sperm maturation The comet assay The sperm chromatin dispersion test The sperm chromatin structure assay Tunel It is well known that transit through the epididymis involves an increase in the compaction of sperm chromatin, which acquires fully condensed status at the caput epididymidis. The purpose of this study was to compare the terminal deoxyribonucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) assay, the comet assay, the sperm chromatin structure assay (SCSA) and the sperm chromatin dispersion (SCD) test by analysing spermatozoa from the caput and cauda epididymidis in order to demonstrate the ability of each technique to discriminate between different degrees of sperm maturity related to chromatin compaction and DNA fragmentation. Our results suggest that some populations of DNA-fragmented spermatozoa associated with immature sperm can only be identified using the comet assay and the SCSA but not with the SCD test or the TUNEL assay. © 2012 AJA, SIMM & SJTU. All rights reserved. 2023-02-20T07:30:44Z 2023-02-20T07:30:44Z 2012 artículo Asian Journal of Andrology 14: 335-337 (2012) 1008-682X http://hdl.handle.net/20.500.12792/3433 http://hdl.handle.net/10261/292620 10.1038/aja.2011.119 1745-7262 en open Medknow Publications |
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DNA damage Mouse epididymis Sperm chromatin Sperm maturation The comet assay The sperm chromatin dispersion test The sperm chromatin structure assay Tunel DNA damage Mouse epididymis Sperm chromatin Sperm maturation The comet assay The sperm chromatin dispersion test The sperm chromatin structure assay Tunel |
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DNA damage Mouse epididymis Sperm chromatin Sperm maturation The comet assay The sperm chromatin dispersion test The sperm chromatin structure assay Tunel DNA damage Mouse epididymis Sperm chromatin Sperm maturation The comet assay The sperm chromatin dispersion test The sperm chromatin structure assay Tunel Pérez Cerezales, Serafín Miranda, A. Gutiérrez Adán, Alfonso Comparison of four methods to evaluate sperm DNA integrity between mouse caput and cauda epididymidis |
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It is well known that transit through the epididymis involves an increase in the compaction of sperm chromatin, which acquires fully condensed status at the caput epididymidis. The purpose of this study was to compare the terminal deoxyribonucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) assay, the comet assay, the sperm chromatin structure assay (SCSA) and the sperm chromatin dispersion (SCD) test by analysing spermatozoa from the caput and cauda epididymidis in order to demonstrate the ability of each technique to discriminate between different degrees of sperm maturity related to chromatin compaction and DNA fragmentation. Our results suggest that some populations of DNA-fragmented spermatozoa associated with immature sperm can only be identified using the comet assay and the SCSA but not with the SCD test or the TUNEL assay. © 2012 AJA, SIMM & SJTU. All rights reserved. |
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artículo |
topic_facet |
DNA damage Mouse epididymis Sperm chromatin Sperm maturation The comet assay The sperm chromatin dispersion test The sperm chromatin structure assay Tunel |
author |
Pérez Cerezales, Serafín Miranda, A. Gutiérrez Adán, Alfonso |
author_facet |
Pérez Cerezales, Serafín Miranda, A. Gutiérrez Adán, Alfonso |
author_sort |
Pérez Cerezales, Serafín |
title |
Comparison of four methods to evaluate sperm DNA integrity between mouse caput and cauda epididymidis |
title_short |
Comparison of four methods to evaluate sperm DNA integrity between mouse caput and cauda epididymidis |
title_full |
Comparison of four methods to evaluate sperm DNA integrity between mouse caput and cauda epididymidis |
title_fullStr |
Comparison of four methods to evaluate sperm DNA integrity between mouse caput and cauda epididymidis |
title_full_unstemmed |
Comparison of four methods to evaluate sperm DNA integrity between mouse caput and cauda epididymidis |
title_sort |
comparison of four methods to evaluate sperm dna integrity between mouse caput and cauda epididymidis |
publisher |
Medknow Publications |
publishDate |
2012 |
url |
http://hdl.handle.net/20.500.12792/3433 http://hdl.handle.net/10261/292620 |
work_keys_str_mv |
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