Short- and long-term outcomes of the absence of protein during bovine blastocyst formation in vitro
In cattle, individual in vitro embryo culture after Day 6 benefits development, allowing non-invasive analysis of culture medium. However, undefined supplements in culture reduce analytical reliability. In this study we assayed the short- and long-term performance of embryos after bovine serum albumin removal over a 24-h period in individual culture. The absence of protein decreased embryo development and cell counts in the inner cell mass without affecting blastocyst sex ratio. However, the absence of protein produced embryos with an improved tendency to survive vitrification after 24h in culture (P≤0.07). After transfer to recipients, birth rates of embryos that had been cultured with protein tended to decrease (P<0.06) mostly as a result of a higher number of miscarriages (P<0.013), reflecting lower viability. Birthweight, gestation length, height and thorax circumference did not differ between embryos cultured with or without protein. In fresh blastocysts cultured without protein, gene expression analysis showed higher abundance (P<0.05) of insulin-like growth factor 2 receptor (IGF2R; imprinting) and activating transcription factor 4 (ATF4) and DNA-damage-inducible transcript 3 (DDIT3; endoplasmic reticulum stress) transcripts, with DNA methyltransferase 3A (DNMT3A; imprinting) tending to increase (P≤0.062). However, in hatched blastocysts that survived cryopreservation, glucose-6-phosphate dehydrogenase (G6PD) was overexpressed in embryos cultured without protein (P<0.01). The absence of protein results in fewer blastocysts but improved long-term viability after cryopreservation. © 2017 CSIRO.
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2017
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| Subjects: | Embryo, Gene expression, Pregnancy, |
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dig-inia-es-10261-2925312023-02-20T07:29:53Z Short- and long-term outcomes of the absence of protein during bovine blastocyst formation in vitro Murillo-Ríos, A. Maillo, V. Muñoz Muñoz, María Gutiérrez Adán, Alfonso Carrocera, S. Martín-González, D. Fernandez-Buznego, A. Gómez, E. Embryo Gene expression Pregnancy In cattle, individual in vitro embryo culture after Day 6 benefits development, allowing non-invasive analysis of culture medium. However, undefined supplements in culture reduce analytical reliability. In this study we assayed the short- and long-term performance of embryos after bovine serum albumin removal over a 24-h period in individual culture. The absence of protein decreased embryo development and cell counts in the inner cell mass without affecting blastocyst sex ratio. However, the absence of protein produced embryos with an improved tendency to survive vitrification after 24h in culture (P≤0.07). After transfer to recipients, birth rates of embryos that had been cultured with protein tended to decrease (P<0.06) mostly as a result of a higher number of miscarriages (P<0.013), reflecting lower viability. Birthweight, gestation length, height and thorax circumference did not differ between embryos cultured with or without protein. In fresh blastocysts cultured without protein, gene expression analysis showed higher abundance (P<0.05) of insulin-like growth factor 2 receptor (IGF2R; imprinting) and activating transcription factor 4 (ATF4) and DNA-damage-inducible transcript 3 (DDIT3; endoplasmic reticulum stress) transcripts, with DNA methyltransferase 3A (DNMT3A; imprinting) tending to increase (P≤0.062). However, in hatched blastocysts that survived cryopreservation, glucose-6-phosphate dehydrogenase (G6PD) was overexpressed in embryos cultured without protein (P<0.01). The absence of protein results in fewer blastocysts but improved long-term viability after cryopreservation. © 2017 CSIRO. 2023-02-20T07:29:53Z 2023-02-20T07:29:53Z 2017 artículo Reproduction, Fertility and Development 29(6): 1064-1073(2017) 1031-3613 http://hdl.handle.net/20.500.12792/3078 http://hdl.handle.net/10261/292531 10.1071/RD15485 1448-5990 en none CSIRO Publishing |
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Embryo Gene expression Pregnancy Embryo Gene expression Pregnancy Murillo-Ríos, A. Maillo, V. Muñoz Muñoz, María Gutiérrez Adán, Alfonso Carrocera, S. Martín-González, D. Fernandez-Buznego, A. Gómez, E. Short- and long-term outcomes of the absence of protein during bovine blastocyst formation in vitro |
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In cattle, individual in vitro embryo culture after Day 6 benefits development, allowing non-invasive analysis of culture medium. However, undefined supplements in culture reduce analytical reliability. In this study we assayed the short- and long-term performance of embryos after bovine serum albumin removal over a 24-h period in individual culture. The absence of protein decreased embryo development and cell counts in the inner cell mass without affecting blastocyst sex ratio. However, the absence of protein produced embryos with an improved tendency to survive vitrification after 24h in culture (P≤0.07). After transfer to recipients, birth rates of embryos that had been cultured with protein tended to decrease (P<0.06) mostly as a result of a higher number of miscarriages (P<0.013), reflecting lower viability. Birthweight, gestation length, height and thorax circumference did not differ between embryos cultured with or without protein. In fresh blastocysts cultured without protein, gene expression analysis showed higher abundance (P<0.05) of insulin-like growth factor 2 receptor (IGF2R; imprinting) and activating transcription factor 4 (ATF4) and DNA-damage-inducible transcript 3 (DDIT3; endoplasmic reticulum stress) transcripts, with DNA methyltransferase 3A (DNMT3A; imprinting) tending to increase (P≤0.062). However, in hatched blastocysts that survived cryopreservation, glucose-6-phosphate dehydrogenase (G6PD) was overexpressed in embryos cultured without protein (P<0.01). The absence of protein results in fewer blastocysts but improved long-term viability after cryopreservation. © 2017 CSIRO. |
| format |
artículo |
| topic_facet |
Embryo Gene expression Pregnancy |
| author |
Murillo-Ríos, A. Maillo, V. Muñoz Muñoz, María Gutiérrez Adán, Alfonso Carrocera, S. Martín-González, D. Fernandez-Buznego, A. Gómez, E. |
| author_facet |
Murillo-Ríos, A. Maillo, V. Muñoz Muñoz, María Gutiérrez Adán, Alfonso Carrocera, S. Martín-González, D. Fernandez-Buznego, A. Gómez, E. |
| author_sort |
Murillo-Ríos, A. |
| title |
Short- and long-term outcomes of the absence of protein during bovine blastocyst formation in vitro |
| title_short |
Short- and long-term outcomes of the absence of protein during bovine blastocyst formation in vitro |
| title_full |
Short- and long-term outcomes of the absence of protein during bovine blastocyst formation in vitro |
| title_fullStr |
Short- and long-term outcomes of the absence of protein during bovine blastocyst formation in vitro |
| title_full_unstemmed |
Short- and long-term outcomes of the absence of protein during bovine blastocyst formation in vitro |
| title_sort |
short- and long-term outcomes of the absence of protein during bovine blastocyst formation in vitro |
| publisher |
CSIRO Publishing |
| publishDate |
2017 |
| url |
http://hdl.handle.net/20.500.12792/3078 http://hdl.handle.net/10261/292531 |
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