Administration of single short-acting doses of GnRH antagonist modifies pituitary and follicular function in sheep
Current study determined the effect of two different single subcutaneous doses (1.5 and 3 mg) of GnRH antagonist (GnRHa) on pituitary and follicular function in non-lactating cyclic ewes. Both doses abolished the pulsatile secretion of luteinizing hormone (LH) for at least 3 days and decreased mean LH concentration during 6 days (0.64 ± 0.09 for control and 0.54 ± 0.05, P < 0.005, and 0.46 ± 0.02, P < 0.00001, for 1.5 and 3 mg, respectively). Supply of GnRHa decreased the number of large dominant follicles, so the total number of smaller follicles, 2-3 mm in size, increased in both treated groups from day 0, reaching its maximum at day 2 in ewes treated with 1.5 mg (19.83 ± 1.05 versus 5.83 ± 0.50 in the control, P < 0.005) and at day 4 in sheep treated with 3 mg (18.67 ± 0.65 versus 5.50 ± 0.65 in the control, P < 0.0001). However, the analysis of follicular function in terms of inhibin A indicated a possible effect of the higher dose of GnRHa on follicular function. The pattern of inhibin secretion in the group treated with 3 mg of GnRHa decreased after the first 48 h, reaching its lowest value on day 4.5 (182.59 ± 3.75 to 140.28 ± 9.91 pg/ml, P < 0.05) concentration significant lower than control sheep (171.93 ± 6.21 pg/ml, P ± 0.01) or treated with 1.5 mg (168.04 ± 7.16 pg/ml, P ± 0.05). Hence, the use of 1.5 mg would be more suitable to induce the presence of a high number of follicles able to grow to preovulatory sizes. © 2005 Elsevier Inc. All rights reserved.
Main Authors: | , , , , , , |
---|---|
Format: | artículo biblioteca |
Language: | English |
Published: |
Elsevier
2005
|
Subjects: | Follicle, GnRH analogues, Gonadotrophins, Inhibin A, Sheep, |
Online Access: | http://hdl.handle.net/20.500.12792/2448 http://hdl.handle.net/10261/292103 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-inia-es-10261-292103 |
---|---|
record_format |
koha |
spelling |
dig-inia-es-10261-2921032023-02-20T07:25:34Z Administration of single short-acting doses of GnRH antagonist modifies pituitary and follicular function in sheep Lopez-Alonso, C. Encinas, T. Garcia-Garcia, R. M. Veiga-Lopez, A. Ros, J. M. McNeilly, A. S. González De Bulnes, Antonio Follicle GnRH analogues Gonadotrophins Inhibin A Sheep Current study determined the effect of two different single subcutaneous doses (1.5 and 3 mg) of GnRH antagonist (GnRHa) on pituitary and follicular function in non-lactating cyclic ewes. Both doses abolished the pulsatile secretion of luteinizing hormone (LH) for at least 3 days and decreased mean LH concentration during 6 days (0.64 ± 0.09 for control and 0.54 ± 0.05, P < 0.005, and 0.46 ± 0.02, P < 0.00001, for 1.5 and 3 mg, respectively). Supply of GnRHa decreased the number of large dominant follicles, so the total number of smaller follicles, 2-3 mm in size, increased in both treated groups from day 0, reaching its maximum at day 2 in ewes treated with 1.5 mg (19.83 ± 1.05 versus 5.83 ± 0.50 in the control, P < 0.005) and at day 4 in sheep treated with 3 mg (18.67 ± 0.65 versus 5.50 ± 0.65 in the control, P < 0.0001). However, the analysis of follicular function in terms of inhibin A indicated a possible effect of the higher dose of GnRHa on follicular function. The pattern of inhibin secretion in the group treated with 3 mg of GnRHa decreased after the first 48 h, reaching its lowest value on day 4.5 (182.59 ± 3.75 to 140.28 ± 9.91 pg/ml, P < 0.05) concentration significant lower than control sheep (171.93 ± 6.21 pg/ml, P ± 0.01) or treated with 1.5 mg (168.04 ± 7.16 pg/ml, P ± 0.05). Hence, the use of 1.5 mg would be more suitable to induce the presence of a high number of follicles able to grow to preovulatory sizes. © 2005 Elsevier Inc. All rights reserved. 2023-02-20T07:25:34Z 2023-02-20T07:25:34Z 2005 artículo Domestic Animal Endocrinology 29(3): 476-487 (2005) 0739-7240 http://hdl.handle.net/20.500.12792/2448 http://hdl.handle.net/10261/292103 10.1016/j.domaniend.2005.02.014 1879-0054 en none Elsevier |
institution |
INIA ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-inia-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del INIA España |
language |
English |
topic |
Follicle GnRH analogues Gonadotrophins Inhibin A Sheep Follicle GnRH analogues Gonadotrophins Inhibin A Sheep |
spellingShingle |
Follicle GnRH analogues Gonadotrophins Inhibin A Sheep Follicle GnRH analogues Gonadotrophins Inhibin A Sheep Lopez-Alonso, C. Encinas, T. Garcia-Garcia, R. M. Veiga-Lopez, A. Ros, J. M. McNeilly, A. S. González De Bulnes, Antonio Administration of single short-acting doses of GnRH antagonist modifies pituitary and follicular function in sheep |
description |
Current study determined the effect of two different single subcutaneous doses (1.5 and 3 mg) of GnRH antagonist (GnRHa) on pituitary and follicular function in non-lactating cyclic ewes. Both doses abolished the pulsatile secretion of luteinizing hormone (LH) for at least 3 days and decreased mean LH concentration during 6 days (0.64 ± 0.09 for control and 0.54 ± 0.05, P < 0.005, and 0.46 ± 0.02, P < 0.00001, for 1.5 and 3 mg, respectively). Supply of GnRHa decreased the number of large dominant follicles, so the total number of smaller follicles, 2-3 mm in size, increased in both treated groups from day 0, reaching its maximum at day 2 in ewes treated with 1.5 mg (19.83 ± 1.05 versus 5.83 ± 0.50 in the control, P < 0.005) and at day 4 in sheep treated with 3 mg (18.67 ± 0.65 versus 5.50 ± 0.65 in the control, P < 0.0001). However, the analysis of follicular function in terms of inhibin A indicated a possible effect of the higher dose of GnRHa on follicular function. The pattern of inhibin secretion in the group treated with 3 mg of GnRHa decreased after the first 48 h, reaching its lowest value on day 4.5 (182.59 ± 3.75 to 140.28 ± 9.91 pg/ml, P < 0.05) concentration significant lower than control sheep (171.93 ± 6.21 pg/ml, P ± 0.01) or treated with 1.5 mg (168.04 ± 7.16 pg/ml, P ± 0.05). Hence, the use of 1.5 mg would be more suitable to induce the presence of a high number of follicles able to grow to preovulatory sizes. © 2005 Elsevier Inc. All rights reserved. |
format |
artículo |
topic_facet |
Follicle GnRH analogues Gonadotrophins Inhibin A Sheep |
author |
Lopez-Alonso, C. Encinas, T. Garcia-Garcia, R. M. Veiga-Lopez, A. Ros, J. M. McNeilly, A. S. González De Bulnes, Antonio |
author_facet |
Lopez-Alonso, C. Encinas, T. Garcia-Garcia, R. M. Veiga-Lopez, A. Ros, J. M. McNeilly, A. S. González De Bulnes, Antonio |
author_sort |
Lopez-Alonso, C. |
title |
Administration of single short-acting doses of GnRH antagonist modifies pituitary and follicular function in sheep |
title_short |
Administration of single short-acting doses of GnRH antagonist modifies pituitary and follicular function in sheep |
title_full |
Administration of single short-acting doses of GnRH antagonist modifies pituitary and follicular function in sheep |
title_fullStr |
Administration of single short-acting doses of GnRH antagonist modifies pituitary and follicular function in sheep |
title_full_unstemmed |
Administration of single short-acting doses of GnRH antagonist modifies pituitary and follicular function in sheep |
title_sort |
administration of single short-acting doses of gnrh antagonist modifies pituitary and follicular function in sheep |
publisher |
Elsevier |
publishDate |
2005 |
url |
http://hdl.handle.net/20.500.12792/2448 http://hdl.handle.net/10261/292103 |
work_keys_str_mv |
AT lopezalonsoc administrationofsingleshortactingdosesofgnrhantagonistmodifiespituitaryandfollicularfunctioninsheep AT encinast administrationofsingleshortactingdosesofgnrhantagonistmodifiespituitaryandfollicularfunctioninsheep AT garciagarciarm administrationofsingleshortactingdosesofgnrhantagonistmodifiespituitaryandfollicularfunctioninsheep AT veigalopeza administrationofsingleshortactingdosesofgnrhantagonistmodifiespituitaryandfollicularfunctioninsheep AT rosjm administrationofsingleshortactingdosesofgnrhantagonistmodifiespituitaryandfollicularfunctioninsheep AT mcneillyas administrationofsingleshortactingdosesofgnrhantagonistmodifiespituitaryandfollicularfunctioninsheep AT gonzalezdebulnesantonio administrationofsingleshortactingdosesofgnrhantagonistmodifiespituitaryandfollicularfunctioninsheep |
_version_ |
1767603298927378432 |