Rapid and differential diagnosis of foot-and-mouth disease, swine vesicular disease, and vesicular stomatitis by a new multiplex RT-PCR assay

Rapid and differential diagnosis of foot-and-mouth disease, swine vesicular disease, and vesicular stomatitis by a new multiplex RT-PCR assay

Show other versions (1)

A highly sensitive and specific one-step multiplex RT-PCR assay has been developed and standardised for the simultaneous and differential detection of the most important vesicular viruses affecting livestock foot-and-mouth disease virus (FMDV), swine vesicular disease virus (SVDV), and vesicular stomatitis virus (VSV). The method uses three primer sets, each one specific for the corresponding virus, selected to detect of all serotypes of FMD and VS. The detection range was confirmed by examination of a collection of 31 isolates of the three target viruses. The specificity of the assay was also demonstrated by testing other related viruses, uninfected cell line cultures and healthy pig tissues. The testing of blood and serum samples from animals infected experimentally proved that the method can be useful for early diagnosis of the diseases, even before the first vesicular lesions are visualized in the infected pigs. An assessment of the performance of the multiplex RT-PCR was carried out using a panel of more than 100 samples from animals infected experimentally, showing the suitability of the method for a rapid (less than 6 h), sensitive and specific differential diagnosis in clinical samples. Additionally, a uniplex RT-PCR for VSV, that amplifies the two viral serotypes, was also developed and tested as a rapid tool for the diagnosis of this vesicular disease. © 2007 Elsevier B.V. All rights reserved.

Saved in:
Bibliographic Details
Main Authors: Fernández, J., Agüero, M., Romero, L., Sánchez, C., Belák, S., Arias, M., Sánchez-Vizcaíno, J. M.
Format: artículo biblioteca
Language:English
Published: Elsevier 2008
Subjects:Foot-and-mouth disease, Swine vesicular disease, Vesicular stomatitis, Differential diagnosis, Multiplex RT-PCR,
Online Access:http://hdl.handle.net/20.500.12792/1347
http://hdl.handle.net/10261/292093
Tags: Add Tag
No Tags, Be the first to tag this record!
id dig-inia-es-10261-292093
record_format koha
spelling dig-inia-es-10261-2920932023-02-20T07:25:28Z Rapid and differential diagnosis of foot-and-mouth disease, swine vesicular disease, and vesicular stomatitis by a new multiplex RT-PCR assay Fernández, J. Agüero, M. Romero, L. Sánchez, C. Belák, S. Arias, M. Sánchez-Vizcaíno, J. M. Foot-and-mouth disease Swine vesicular disease Vesicular stomatitis Differential diagnosis Multiplex RT-PCR A highly sensitive and specific one-step multiplex RT-PCR assay has been developed and standardised for the simultaneous and differential detection of the most important vesicular viruses affecting livestock foot-and-mouth disease virus (FMDV), swine vesicular disease virus (SVDV), and vesicular stomatitis virus (VSV). The method uses three primer sets, each one specific for the corresponding virus, selected to detect of all serotypes of FMD and VS. The detection range was confirmed by examination of a collection of 31 isolates of the three target viruses. The specificity of the assay was also demonstrated by testing other related viruses, uninfected cell line cultures and healthy pig tissues. The testing of blood and serum samples from animals infected experimentally proved that the method can be useful for early diagnosis of the diseases, even before the first vesicular lesions are visualized in the infected pigs. An assessment of the performance of the multiplex RT-PCR was carried out using a panel of more than 100 samples from animals infected experimentally, showing the suitability of the method for a rapid (less than 6 h), sensitive and specific differential diagnosis in clinical samples. Additionally, a uniplex RT-PCR for VSV, that amplifies the two viral serotypes, was also developed and tested as a rapid tool for the diagnosis of this vesicular disease. © 2007 Elsevier B.V. All rights reserved. 2023-02-20T07:25:28Z 2023-02-20T07:25:28Z 2008 artículo Journal of Virological Methods 147(2): 301-311 (2008) 0166-0934 http://hdl.handle.net/20.500.12792/1347 http://hdl.handle.net/10261/292093 10.1016/j.jviromet.2007.09.010 en none Elsevier
institution INIA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-inia-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del INIA España
language English
topic Foot-and-mouth disease
Swine vesicular disease
Vesicular stomatitis
Differential diagnosis
Multiplex RT-PCR
Foot-and-mouth disease
Swine vesicular disease
Vesicular stomatitis
Differential diagnosis
Multiplex RT-PCR
spellingShingle Foot-and-mouth disease
Swine vesicular disease
Vesicular stomatitis
Differential diagnosis
Multiplex RT-PCR
Foot-and-mouth disease
Swine vesicular disease
Vesicular stomatitis
Differential diagnosis
Multiplex RT-PCR
Fernández, J.
Agüero, M.
Romero, L.
Sánchez, C.
Belák, S.
Arias, M.
Sánchez-Vizcaíno, J. M.
Rapid and differential diagnosis of foot-and-mouth disease, swine vesicular disease, and vesicular stomatitis by a new multiplex RT-PCR assay
description A highly sensitive and specific one-step multiplex RT-PCR assay has been developed and standardised for the simultaneous and differential detection of the most important vesicular viruses affecting livestock foot-and-mouth disease virus (FMDV), swine vesicular disease virus (SVDV), and vesicular stomatitis virus (VSV). The method uses three primer sets, each one specific for the corresponding virus, selected to detect of all serotypes of FMD and VS. The detection range was confirmed by examination of a collection of 31 isolates of the three target viruses. The specificity of the assay was also demonstrated by testing other related viruses, uninfected cell line cultures and healthy pig tissues. The testing of blood and serum samples from animals infected experimentally proved that the method can be useful for early diagnosis of the diseases, even before the first vesicular lesions are visualized in the infected pigs. An assessment of the performance of the multiplex RT-PCR was carried out using a panel of more than 100 samples from animals infected experimentally, showing the suitability of the method for a rapid (less than 6 h), sensitive and specific differential diagnosis in clinical samples. Additionally, a uniplex RT-PCR for VSV, that amplifies the two viral serotypes, was also developed and tested as a rapid tool for the diagnosis of this vesicular disease. © 2007 Elsevier B.V. All rights reserved.
format artículo
topic_facet Foot-and-mouth disease
Swine vesicular disease
Vesicular stomatitis
Differential diagnosis
Multiplex RT-PCR
author Fernández, J.
Agüero, M.
Romero, L.
Sánchez, C.
Belák, S.
Arias, M.
Sánchez-Vizcaíno, J. M.
author_facet Fernández, J.
Agüero, M.
Romero, L.
Sánchez, C.
Belák, S.
Arias, M.
Sánchez-Vizcaíno, J. M.
author_sort Fernández, J.
title Rapid and differential diagnosis of foot-and-mouth disease, swine vesicular disease, and vesicular stomatitis by a new multiplex RT-PCR assay
title_short Rapid and differential diagnosis of foot-and-mouth disease, swine vesicular disease, and vesicular stomatitis by a new multiplex RT-PCR assay
title_full Rapid and differential diagnosis of foot-and-mouth disease, swine vesicular disease, and vesicular stomatitis by a new multiplex RT-PCR assay
title_fullStr Rapid and differential diagnosis of foot-and-mouth disease, swine vesicular disease, and vesicular stomatitis by a new multiplex RT-PCR assay
title_full_unstemmed Rapid and differential diagnosis of foot-and-mouth disease, swine vesicular disease, and vesicular stomatitis by a new multiplex RT-PCR assay
title_sort rapid and differential diagnosis of foot-and-mouth disease, swine vesicular disease, and vesicular stomatitis by a new multiplex rt-pcr assay
publisher Elsevier
publishDate 2008
url http://hdl.handle.net/20.500.12792/1347
http://hdl.handle.net/10261/292093
work_keys_str_mv AT fernandezj rapidanddifferentialdiagnosisoffootandmouthdiseaseswinevesiculardiseaseandvesicularstomatitisbyanewmultiplexrtpcrassay
AT aguerom rapidanddifferentialdiagnosisoffootandmouthdiseaseswinevesiculardiseaseandvesicularstomatitisbyanewmultiplexrtpcrassay
AT romerol rapidanddifferentialdiagnosisoffootandmouthdiseaseswinevesiculardiseaseandvesicularstomatitisbyanewmultiplexrtpcrassay
AT sanchezc rapidanddifferentialdiagnosisoffootandmouthdiseaseswinevesiculardiseaseandvesicularstomatitisbyanewmultiplexrtpcrassay
AT belaks rapidanddifferentialdiagnosisoffootandmouthdiseaseswinevesiculardiseaseandvesicularstomatitisbyanewmultiplexrtpcrassay
AT ariasm rapidanddifferentialdiagnosisoffootandmouthdiseaseswinevesiculardiseaseandvesicularstomatitisbyanewmultiplexrtpcrassay
AT sanchezvizcainojm rapidanddifferentialdiagnosisoffootandmouthdiseaseswinevesiculardiseaseandvesicularstomatitisbyanewmultiplexrtpcrassay
_version_ 1767603297584152576

Similar Items