Set of vectors for the expression of histidine-tagged proteins in vaccinia virus recombinants
Vaccinia virus expression vectors are widely used to direct the expression of proteins in eukaryotic cells. Here, we describe a new set of plasmid vectors designed for the expression of histidine-tagged proteins in the vaccinia system. To facilitate the rapid isolation of virus recombinants, the plasmids contain a viral gene (F13L) that serves as an efficient selection marker based on virus plaque phenoytpe. Histidine codons and restriction sites derived from pET-16b bacterial expression plasmid were included, thus facilitating the transfer of genes between E. coli and vaccinia expression plasmids. Plasmids in which the gene is placed downstream of either a strong vaccinia virus or a T7 promoter were constructed, allowing for constitutive or conditional expression, respectively, of the foreign protein.
Main Authors: | Galindo Barreales, Inmaculada, Lorenzo Gilsanz, María Mar, Blasco Lozano, Rafael |
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Format: | artículo biblioteca |
Language: | English |
Published: |
Future Science
2001
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Online Access: | http://hdl.handle.net/20.500.12792/1501 http://hdl.handle.net/10261/291927 |
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