Follicular growth, endocrine response and embryo yields in sheep superovulated with FSH after pretreatment with a single short-acting dose of GnRH antagonist
The objective of this study was to characterize follicular development, onset of oestrus and preovulatory LH surge, and in vivo embryo yields of sheep superovulated after treatment with a single dose of 1.5 mg of GnRH antagonist (GnRHa). At first FSH dose, ewes treated with GnRH antagonist (n = 12) showed a higher number of gonadotrophin-responsive follicles, 2-3 mm, than control ewes (n = 9, 13.5 ± 3.8 versus 5.3 ± 0.3, P < 0.05). Administration of FSH increased the number of ≥4 mm follicles at sponge removal in both groups (19.3 ± 3.8, P < 0.0005 for treated ewes and 12.7 ± 5.4, P < 0.01 for controls). Thereafter, a 25% of the GnRHa-treated sheep did not show oestrous behaviour whilst none control sheep failed (P = 0.06). The preovulatory LH surge was detected in an 88.9% of control ewes and 66.7% of GnRHa-treated sheep. A 77.8% of control females showed ovulation with a mean of 9.6 ± 0.9 CL and 3.3 ± 0.7 viable embryos, while ewes treated with GnRHa and showing an LH surge exhibited a bimodal distribution of response; 50% showed no ovulatory response and 50% superovulated with a mean of 12.2 ± 1.1 CL and 7.3 ± 1.1 viable embryos. In conclusion, a single dose of GnRHa enhances the number of gonadotrophin-dependent follicles able to grow to preovulatory sizes in response to an FSH supply. However, LH secretion may be altered in some females, which can affect the preovulatory LH surge and/or can weak the terminal maturation of ovulatory follicles. © 2005 Elsevier Inc. All rights reserved.
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Elsevier
2005
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Subjects: | Follicle, GnRH analogues, Sheep, Superovulation, |
Online Access: | http://hdl.handle.net/20.500.12792/2449 http://hdl.handle.net/10261/291861 |
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dig-inia-es-10261-2918612023-02-20T07:23:00Z Follicular growth, endocrine response and embryo yields in sheep superovulated with FSH after pretreatment with a single short-acting dose of GnRH antagonist Lopez-Alonso, C. Encinas, T. Veiga-Lopez, A. Garcia-Garcia, R. M. Cocero Oviedo, María Jesús Ros, J. M. McNeilly, A. S. González De Bulnes, Antonio Follicle GnRH analogues Sheep Superovulation The objective of this study was to characterize follicular development, onset of oestrus and preovulatory LH surge, and in vivo embryo yields of sheep superovulated after treatment with a single dose of 1.5 mg of GnRH antagonist (GnRHa). At first FSH dose, ewes treated with GnRH antagonist (n = 12) showed a higher number of gonadotrophin-responsive follicles, 2-3 mm, than control ewes (n = 9, 13.5 ± 3.8 versus 5.3 ± 0.3, P < 0.05). Administration of FSH increased the number of ≥4 mm follicles at sponge removal in both groups (19.3 ± 3.8, P < 0.0005 for treated ewes and 12.7 ± 5.4, P < 0.01 for controls). Thereafter, a 25% of the GnRHa-treated sheep did not show oestrous behaviour whilst none control sheep failed (P = 0.06). The preovulatory LH surge was detected in an 88.9% of control ewes and 66.7% of GnRHa-treated sheep. A 77.8% of control females showed ovulation with a mean of 9.6 ± 0.9 CL and 3.3 ± 0.7 viable embryos, while ewes treated with GnRHa and showing an LH surge exhibited a bimodal distribution of response; 50% showed no ovulatory response and 50% superovulated with a mean of 12.2 ± 1.1 CL and 7.3 ± 1.1 viable embryos. In conclusion, a single dose of GnRHa enhances the number of gonadotrophin-dependent follicles able to grow to preovulatory sizes in response to an FSH supply. However, LH secretion may be altered in some females, which can affect the preovulatory LH surge and/or can weak the terminal maturation of ovulatory follicles. © 2005 Elsevier Inc. All rights reserved. 2023-02-20T07:23:00Z 2023-02-20T07:23:00Z 2005 artículo Theriogenology 64(8): 1833-1843 (2005) 0093-691X http://hdl.handle.net/20.500.12792/2449 http://hdl.handle.net/10261/291861 10.1016/j.theriogenology.2005.04.021 1879-3231 en none Elsevier |
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Follicle GnRH analogues Sheep Superovulation Follicle GnRH analogues Sheep Superovulation |
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Follicle GnRH analogues Sheep Superovulation Follicle GnRH analogues Sheep Superovulation Lopez-Alonso, C. Encinas, T. Veiga-Lopez, A. Garcia-Garcia, R. M. Cocero Oviedo, María Jesús Ros, J. M. McNeilly, A. S. González De Bulnes, Antonio Follicular growth, endocrine response and embryo yields in sheep superovulated with FSH after pretreatment with a single short-acting dose of GnRH antagonist |
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The objective of this study was to characterize follicular development, onset of oestrus and preovulatory LH surge, and in vivo embryo yields of sheep superovulated after treatment with a single dose of 1.5 mg of GnRH antagonist (GnRHa). At first FSH dose, ewes treated with GnRH antagonist (n = 12) showed a higher number of gonadotrophin-responsive follicles, 2-3 mm, than control ewes (n = 9, 13.5 ± 3.8 versus 5.3 ± 0.3, P < 0.05). Administration of FSH increased the number of ≥4 mm follicles at sponge removal in both groups (19.3 ± 3.8, P < 0.0005 for treated ewes and 12.7 ± 5.4, P < 0.01 for controls). Thereafter, a 25% of the GnRHa-treated sheep did not show oestrous behaviour whilst none control sheep failed (P = 0.06). The preovulatory LH surge was detected in an 88.9% of control ewes and 66.7% of GnRHa-treated sheep. A 77.8% of control females showed ovulation with a mean of 9.6 ± 0.9 CL and 3.3 ± 0.7 viable embryos, while ewes treated with GnRHa and showing an LH surge exhibited a bimodal distribution of response; 50% showed no ovulatory response and 50% superovulated with a mean of 12.2 ± 1.1 CL and 7.3 ± 1.1 viable embryos. In conclusion, a single dose of GnRHa enhances the number of gonadotrophin-dependent follicles able to grow to preovulatory sizes in response to an FSH supply. However, LH secretion may be altered in some females, which can affect the preovulatory LH surge and/or can weak the terminal maturation of ovulatory follicles. © 2005 Elsevier Inc. All rights reserved. |
format |
artículo |
topic_facet |
Follicle GnRH analogues Sheep Superovulation |
author |
Lopez-Alonso, C. Encinas, T. Veiga-Lopez, A. Garcia-Garcia, R. M. Cocero Oviedo, María Jesús Ros, J. M. McNeilly, A. S. González De Bulnes, Antonio |
author_facet |
Lopez-Alonso, C. Encinas, T. Veiga-Lopez, A. Garcia-Garcia, R. M. Cocero Oviedo, María Jesús Ros, J. M. McNeilly, A. S. González De Bulnes, Antonio |
author_sort |
Lopez-Alonso, C. |
title |
Follicular growth, endocrine response and embryo yields in sheep superovulated with FSH after pretreatment with a single short-acting dose of GnRH antagonist |
title_short |
Follicular growth, endocrine response and embryo yields in sheep superovulated with FSH after pretreatment with a single short-acting dose of GnRH antagonist |
title_full |
Follicular growth, endocrine response and embryo yields in sheep superovulated with FSH after pretreatment with a single short-acting dose of GnRH antagonist |
title_fullStr |
Follicular growth, endocrine response and embryo yields in sheep superovulated with FSH after pretreatment with a single short-acting dose of GnRH antagonist |
title_full_unstemmed |
Follicular growth, endocrine response and embryo yields in sheep superovulated with FSH after pretreatment with a single short-acting dose of GnRH antagonist |
title_sort |
follicular growth, endocrine response and embryo yields in sheep superovulated with fsh after pretreatment with a single short-acting dose of gnrh antagonist |
publisher |
Elsevier |
publishDate |
2005 |
url |
http://hdl.handle.net/20.500.12792/2449 http://hdl.handle.net/10261/291861 |
work_keys_str_mv |
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