Development and pattern of mRNA relative abundance of bovine embryos cultured in the isolated mouse oviduct in organ culture
The aim of this study was to examine the development of bovine zygotes in isolated mouse oviducts (IMO) and the quality of the blastocysts produced. In vitro produced bovine zygotes were transferred into the ampullae of the IMO and cultured in SOF or KSOM. Control embryos were cultured in droplets of the same media. Following 6 days of culture, blastocysts were processed for nuclei counts or mRNA abundance. Culture in the IMO did not affect the proportion of zygotes developing to the blastocyst stage compared to the respective control droplets (SOF 17.7 ± 3.2% vs. 18.8± 2.7%; KSOM 20.7 ± 2.6% vs. 22.2 ± 2.8%). Culture in the IMO in KSOM resulted in an increased number of inner cell mass (ICM) nuclei; however, total nuclei number or incidence of apoptosis was unaffected. Culture in the IMO in SOF resulted in an increase (P < 0.05) in abundance of transcripts in blastocysts for Oct-4 and SOX, and reduced abundance of Glut-1, Na/K, Cx43, and survivin compared to blastocysts derived from culture in SOF alone. In contrast, culture in the IMO in KSOM resulted in increased abundance of transcripts for Glut-1, Cx43, Oct-4, and survivin and reduced expression of Na/K and SOX compared to KSOM alone. Transcripts for G6PDH, IFN-τ, and E-Cad were unaffected. These data confirm that the IMO is capable of supporting development of bovine embryos. Depending on the basal medium used, the pattern of transcript abundance in embryos derived from the IMO is similar to that of in vivo derived embryos. © 2006 Wiley-Liss, Inc.
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2007
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Subjects: | IVF, Embryo quality, Organ culture, Gene expression, |
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dig-inia-es-10261-2918212023-02-20T07:22:35Z Development and pattern of mRNA relative abundance of bovine embryos cultured in the isolated mouse oviduct in organ culture Rizos Dimitrios, Dimitrios Pintado, B. De La Fuente, J. Lonergan, P. Gutiérrez Adán, Alfonso IVF Embryo quality Organ culture Gene expression The aim of this study was to examine the development of bovine zygotes in isolated mouse oviducts (IMO) and the quality of the blastocysts produced. In vitro produced bovine zygotes were transferred into the ampullae of the IMO and cultured in SOF or KSOM. Control embryos were cultured in droplets of the same media. Following 6 days of culture, blastocysts were processed for nuclei counts or mRNA abundance. Culture in the IMO did not affect the proportion of zygotes developing to the blastocyst stage compared to the respective control droplets (SOF 17.7 ± 3.2% vs. 18.8± 2.7%; KSOM 20.7 ± 2.6% vs. 22.2 ± 2.8%). Culture in the IMO in KSOM resulted in an increased number of inner cell mass (ICM) nuclei; however, total nuclei number or incidence of apoptosis was unaffected. Culture in the IMO in SOF resulted in an increase (P < 0.05) in abundance of transcripts in blastocysts for Oct-4 and SOX, and reduced abundance of Glut-1, Na/K, Cx43, and survivin compared to blastocysts derived from culture in SOF alone. In contrast, culture in the IMO in KSOM resulted in increased abundance of transcripts for Glut-1, Cx43, Oct-4, and survivin and reduced expression of Na/K and SOX compared to KSOM alone. Transcripts for G6PDH, IFN-τ, and E-Cad were unaffected. These data confirm that the IMO is capable of supporting development of bovine embryos. Depending on the basal medium used, the pattern of transcript abundance in embryos derived from the IMO is similar to that of in vivo derived embryos. © 2006 Wiley-Liss, Inc. 2023-02-20T07:22:35Z 2023-02-20T07:22:35Z 2007 artículo Molecular Reproduction and Development 74(6): 716-723 (2007) 1040-452X http://hdl.handle.net/20.500.12792/3730 http://hdl.handle.net/10261/291821 10.1002/mrd.20652 1098-2795 en none Wiley |
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IVF Embryo quality Organ culture Gene expression IVF Embryo quality Organ culture Gene expression |
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IVF Embryo quality Organ culture Gene expression IVF Embryo quality Organ culture Gene expression Rizos Dimitrios, Dimitrios Pintado, B. De La Fuente, J. Lonergan, P. Gutiérrez Adán, Alfonso Development and pattern of mRNA relative abundance of bovine embryos cultured in the isolated mouse oviduct in organ culture |
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The aim of this study was to examine the development of bovine zygotes in isolated mouse oviducts (IMO) and the quality of the blastocysts produced. In vitro produced bovine zygotes were transferred into the ampullae of the IMO and cultured in SOF or KSOM. Control embryos were cultured in droplets of the same media. Following 6 days of culture, blastocysts were processed for nuclei counts or mRNA abundance. Culture in the IMO did not affect the proportion of zygotes developing to the blastocyst stage compared to the respective control droplets (SOF 17.7 ± 3.2% vs. 18.8± 2.7%; KSOM 20.7 ± 2.6% vs. 22.2 ± 2.8%). Culture in the IMO in KSOM resulted in an increased number of inner cell mass (ICM) nuclei; however, total nuclei number or incidence of apoptosis was unaffected. Culture in the IMO in SOF resulted in an increase (P < 0.05) in abundance of transcripts in blastocysts for Oct-4 and SOX, and reduced abundance of Glut-1, Na/K, Cx43, and survivin compared to blastocysts derived from culture in SOF alone. In contrast, culture in the IMO in KSOM resulted in increased abundance of transcripts for Glut-1, Cx43, Oct-4, and survivin and reduced expression of Na/K and SOX compared to KSOM alone. Transcripts for G6PDH, IFN-τ, and E-Cad were unaffected. These data confirm that the IMO is capable of supporting development of bovine embryos. Depending on the basal medium used, the pattern of transcript abundance in embryos derived from the IMO is similar to that of in vivo derived embryos. © 2006 Wiley-Liss, Inc. |
format |
artículo |
topic_facet |
IVF Embryo quality Organ culture Gene expression |
author |
Rizos Dimitrios, Dimitrios Pintado, B. De La Fuente, J. Lonergan, P. Gutiérrez Adán, Alfonso |
author_facet |
Rizos Dimitrios, Dimitrios Pintado, B. De La Fuente, J. Lonergan, P. Gutiérrez Adán, Alfonso |
author_sort |
Rizos Dimitrios, Dimitrios |
title |
Development and pattern of mRNA relative abundance of bovine embryos cultured in the isolated mouse oviduct in organ culture |
title_short |
Development and pattern of mRNA relative abundance of bovine embryos cultured in the isolated mouse oviduct in organ culture |
title_full |
Development and pattern of mRNA relative abundance of bovine embryos cultured in the isolated mouse oviduct in organ culture |
title_fullStr |
Development and pattern of mRNA relative abundance of bovine embryos cultured in the isolated mouse oviduct in organ culture |
title_full_unstemmed |
Development and pattern of mRNA relative abundance of bovine embryos cultured in the isolated mouse oviduct in organ culture |
title_sort |
development and pattern of mrna relative abundance of bovine embryos cultured in the isolated mouse oviduct in organ culture |
publisher |
Wiley |
publishDate |
2007 |
url |
http://hdl.handle.net/20.500.12792/3730 http://hdl.handle.net/10261/291821 |
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