Recognition of novel viral sequences that associate with the dynein light chain LC8 identified through a pepscan technique
Recent data from multiple laboratories indicate that upon infection, many different families of viruses hijack the dynein motor machinery and become transported in a retrograde manner towards the cell nucleus. In certain cases, one of the dynein light chains, LC8, is involved in this interaction. Using a library of overlapping dodecapeptides synthesized on a cellulose membrane (pepscan technique) we have analyzed the interaction of the dynein light chain LC8 with 17 polypeptides of viral origin. We demonstrate the strong binding of two herpesvirus polypeptides, the human adenovirus protease, vaccinia virus polymerase, human papillomavirus E4 protein, yam mosaic virus polyprotein, human respiratory syncytial virus attachment glycoprotein, human coxsackievirus capsid protein and the product of the AMV179 gene of an insect poxvirus to LC8. Our data corroborate the manipulation of the dynein macromolecular complex of the cell during viral infection and point towards the light chain LC8 as one of the most frequently used targets of virus manipulation. © 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
Main Authors: | , , , , , , |
---|---|
Format: | artículo biblioteca |
Language: | English |
Published: |
Wiley
2003
|
Subjects: | Migration, Microtubule, Infection, Dynein, Pepscan, |
Online Access: | http://hdl.handle.net/20.500.12792/2761 http://hdl.handle.net/10261/291795 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-inia-es-10261-291795 |
---|---|
record_format |
koha |
spelling |
dig-inia-es-10261-2917952023-02-20T07:22:19Z Recognition of novel viral sequences that associate with the dynein light chain LC8 identified through a pepscan technique Martínez-Moreno, M. Navarro-Lérida, I. Roncal, F. Albar, J. P. Alonso, C. Gavilanes, F. Rodríguez-Crespo, I. Migration Microtubule Infection Dynein Pepscan Recent data from multiple laboratories indicate that upon infection, many different families of viruses hijack the dynein motor machinery and become transported in a retrograde manner towards the cell nucleus. In certain cases, one of the dynein light chains, LC8, is involved in this interaction. Using a library of overlapping dodecapeptides synthesized on a cellulose membrane (pepscan technique) we have analyzed the interaction of the dynein light chain LC8 with 17 polypeptides of viral origin. We demonstrate the strong binding of two herpesvirus polypeptides, the human adenovirus protease, vaccinia virus polymerase, human papillomavirus E4 protein, yam mosaic virus polyprotein, human respiratory syncytial virus attachment glycoprotein, human coxsackievirus capsid protein and the product of the AMV179 gene of an insect poxvirus to LC8. Our data corroborate the manipulation of the dynein macromolecular complex of the cell during viral infection and point towards the light chain LC8 as one of the most frequently used targets of virus manipulation. © 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies. 2023-02-20T07:22:19Z 2023-02-20T07:22:19Z 2003 artículo FEBS Letters 544(17): 262-267 (2003) 0014-5793 http://hdl.handle.net/20.500.12792/2761 http://hdl.handle.net/10261/291795 10.1016/S0014-5793(03)00516-7 1873-3468 en none Wiley |
institution |
INIA ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-inia-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del INIA España |
language |
English |
topic |
Migration Microtubule Infection Dynein Pepscan Migration Microtubule Infection Dynein Pepscan |
spellingShingle |
Migration Microtubule Infection Dynein Pepscan Migration Microtubule Infection Dynein Pepscan Martínez-Moreno, M. Navarro-Lérida, I. Roncal, F. Albar, J. P. Alonso, C. Gavilanes, F. Rodríguez-Crespo, I. Recognition of novel viral sequences that associate with the dynein light chain LC8 identified through a pepscan technique |
description |
Recent data from multiple laboratories indicate that upon infection, many different families of viruses hijack the dynein motor machinery and become transported in a retrograde manner towards the cell nucleus. In certain cases, one of the dynein light chains, LC8, is involved in this interaction. Using a library of overlapping dodecapeptides synthesized on a cellulose membrane (pepscan technique) we have analyzed the interaction of the dynein light chain LC8 with 17 polypeptides of viral origin. We demonstrate the strong binding of two herpesvirus polypeptides, the human adenovirus protease, vaccinia virus polymerase, human papillomavirus E4 protein, yam mosaic virus polyprotein, human respiratory syncytial virus attachment glycoprotein, human coxsackievirus capsid protein and the product of the AMV179 gene of an insect poxvirus to LC8. Our data corroborate the manipulation of the dynein macromolecular complex of the cell during viral infection and point towards the light chain LC8 as one of the most frequently used targets of virus manipulation. © 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies. |
format |
artículo |
topic_facet |
Migration Microtubule Infection Dynein Pepscan |
author |
Martínez-Moreno, M. Navarro-Lérida, I. Roncal, F. Albar, J. P. Alonso, C. Gavilanes, F. Rodríguez-Crespo, I. |
author_facet |
Martínez-Moreno, M. Navarro-Lérida, I. Roncal, F. Albar, J. P. Alonso, C. Gavilanes, F. Rodríguez-Crespo, I. |
author_sort |
Martínez-Moreno, M. |
title |
Recognition of novel viral sequences that associate with the dynein light chain LC8 identified through a pepscan technique |
title_short |
Recognition of novel viral sequences that associate with the dynein light chain LC8 identified through a pepscan technique |
title_full |
Recognition of novel viral sequences that associate with the dynein light chain LC8 identified through a pepscan technique |
title_fullStr |
Recognition of novel viral sequences that associate with the dynein light chain LC8 identified through a pepscan technique |
title_full_unstemmed |
Recognition of novel viral sequences that associate with the dynein light chain LC8 identified through a pepscan technique |
title_sort |
recognition of novel viral sequences that associate with the dynein light chain lc8 identified through a pepscan technique |
publisher |
Wiley |
publishDate |
2003 |
url |
http://hdl.handle.net/20.500.12792/2761 http://hdl.handle.net/10261/291795 |
work_keys_str_mv |
AT martinezmorenom recognitionofnovelviralsequencesthatassociatewiththedyneinlightchainlc8identifiedthroughapepscantechnique AT navarroleridai recognitionofnovelviralsequencesthatassociatewiththedyneinlightchainlc8identifiedthroughapepscantechnique AT roncalf recognitionofnovelviralsequencesthatassociatewiththedyneinlightchainlc8identifiedthroughapepscantechnique AT albarjp recognitionofnovelviralsequencesthatassociatewiththedyneinlightchainlc8identifiedthroughapepscantechnique AT alonsoc recognitionofnovelviralsequencesthatassociatewiththedyneinlightchainlc8identifiedthroughapepscantechnique AT gavilanesf recognitionofnovelviralsequencesthatassociatewiththedyneinlightchainlc8identifiedthroughapepscantechnique AT rodriguezcrespoi recognitionofnovelviralsequencesthatassociatewiththedyneinlightchainlc8identifiedthroughapepscantechnique |
_version_ |
1767603256124506112 |