The influence of washing Spanish ibex (Capra pyrenaica) sperm on the effects of cryopreservation in dependency of the photoperiod
Extenders containing low concentrations of egg yolk are recommended for cryopreserving ibex spermatozoa. However, the phylogenetic relationship of the Spanish ibex (Capra pyrenaica) with domestic goats suggests that phospholipases in the seminal plasma may have a negative effect on the response to freezing-thawing when egg yolk-based diluents are employed. The aim of the current work was to determine how seminal plasma removal from Spanish ibex semen, collected by electroejaculation over a period of 1 yr, affects its response to freezing-thawing. Semen was collected from six adult ibexes maintained in captivity. The negative effects of freezing-thawing on the quality of sperm motility and on the integrity of the acrosome and plasma membrane were more serious in the nonwashed semen samples than in those from which the seminal plasma had been removed (P < 0.01, P < 0.05, and P < 0.05 respectively). The beneficial effect of removing the seminal plasma was particularly noticeable during the time of decreasing photoperiod. This suggests that ibex semen shows increased phospholipase activity during the rutting season. © 2010 Elsevier Inc. All rights reserved.
Main Authors: | , , , |
---|---|
Format: | artículo biblioteca |
Language: | English |
Published: |
Elsevier
2010
|
Subjects: | Egg yolk, Electroejaculation, Goat, Semen, Washing, |
Online Access: | http://hdl.handle.net/20.500.12792/5928 http://hdl.handle.net/10261/291784 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-inia-es-10261-291784 |
---|---|
record_format |
koha |
spelling |
dig-inia-es-10261-2917842023-02-20T07:22:12Z The influence of washing Spanish ibex (Capra pyrenaica) sperm on the effects of cryopreservation in dependency of the photoperiod Coloma, M. A. Toledano Díaz, Adolfo López Sebastián, Antonio Santiago Moreno, Julián Egg yolk Electroejaculation Goat Semen Washing Extenders containing low concentrations of egg yolk are recommended for cryopreserving ibex spermatozoa. However, the phylogenetic relationship of the Spanish ibex (Capra pyrenaica) with domestic goats suggests that phospholipases in the seminal plasma may have a negative effect on the response to freezing-thawing when egg yolk-based diluents are employed. The aim of the current work was to determine how seminal plasma removal from Spanish ibex semen, collected by electroejaculation over a period of 1 yr, affects its response to freezing-thawing. Semen was collected from six adult ibexes maintained in captivity. The negative effects of freezing-thawing on the quality of sperm motility and on the integrity of the acrosome and plasma membrane were more serious in the nonwashed semen samples than in those from which the seminal plasma had been removed (P < 0.01, P < 0.05, and P < 0.05 respectively). The beneficial effect of removing the seminal plasma was particularly noticeable during the time of decreasing photoperiod. This suggests that ibex semen shows increased phospholipase activity during the rutting season. © 2010 Elsevier Inc. All rights reserved. 2023-02-20T07:22:12Z 2023-02-20T07:22:12Z 2010 artículo Theriogenology 73: 900-908 (2010) 0093-691X http://hdl.handle.net/20.500.12792/5928 http://hdl.handle.net/10261/291784 10.1016/j.theriogenology.2009.11.014 1879-3231 en none Elsevier |
institution |
INIA ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-inia-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del INIA España |
language |
English |
topic |
Egg yolk Electroejaculation Goat Semen Washing Egg yolk Electroejaculation Goat Semen Washing |
spellingShingle |
Egg yolk Electroejaculation Goat Semen Washing Egg yolk Electroejaculation Goat Semen Washing Coloma, M. A. Toledano Díaz, Adolfo López Sebastián, Antonio Santiago Moreno, Julián The influence of washing Spanish ibex (Capra pyrenaica) sperm on the effects of cryopreservation in dependency of the photoperiod |
description |
Extenders containing low concentrations of egg yolk are recommended for cryopreserving ibex spermatozoa. However, the phylogenetic relationship of the Spanish ibex (Capra pyrenaica) with domestic goats suggests that phospholipases in the seminal plasma may have a negative effect on the response to freezing-thawing when egg yolk-based diluents are employed. The aim of the current work was to determine how seminal plasma removal from Spanish ibex semen, collected by electroejaculation over a period of 1 yr, affects its response to freezing-thawing. Semen was collected from six adult ibexes maintained in captivity. The negative effects of freezing-thawing on the quality of sperm motility and on the integrity of the acrosome and plasma membrane were more serious in the nonwashed semen samples than in those from which the seminal plasma had been removed (P < 0.01, P < 0.05, and P < 0.05 respectively). The beneficial effect of removing the seminal plasma was particularly noticeable during the time of decreasing photoperiod. This suggests that ibex semen shows increased phospholipase activity during the rutting season. © 2010 Elsevier Inc. All rights reserved. |
format |
artículo |
topic_facet |
Egg yolk Electroejaculation Goat Semen Washing |
author |
Coloma, M. A. Toledano Díaz, Adolfo López Sebastián, Antonio Santiago Moreno, Julián |
author_facet |
Coloma, M. A. Toledano Díaz, Adolfo López Sebastián, Antonio Santiago Moreno, Julián |
author_sort |
Coloma, M. A. |
title |
The influence of washing Spanish ibex (Capra pyrenaica) sperm on the effects of cryopreservation in dependency of the photoperiod |
title_short |
The influence of washing Spanish ibex (Capra pyrenaica) sperm on the effects of cryopreservation in dependency of the photoperiod |
title_full |
The influence of washing Spanish ibex (Capra pyrenaica) sperm on the effects of cryopreservation in dependency of the photoperiod |
title_fullStr |
The influence of washing Spanish ibex (Capra pyrenaica) sperm on the effects of cryopreservation in dependency of the photoperiod |
title_full_unstemmed |
The influence of washing Spanish ibex (Capra pyrenaica) sperm on the effects of cryopreservation in dependency of the photoperiod |
title_sort |
influence of washing spanish ibex (capra pyrenaica) sperm on the effects of cryopreservation in dependency of the photoperiod |
publisher |
Elsevier |
publishDate |
2010 |
url |
http://hdl.handle.net/20.500.12792/5928 http://hdl.handle.net/10261/291784 |
work_keys_str_mv |
AT colomama theinfluenceofwashingspanishibexcaprapyrenaicaspermontheeffectsofcryopreservationindependencyofthephotoperiod AT toledanodiazadolfo theinfluenceofwashingspanishibexcaprapyrenaicaspermontheeffectsofcryopreservationindependencyofthephotoperiod AT lopezsebastianantonio theinfluenceofwashingspanishibexcaprapyrenaicaspermontheeffectsofcryopreservationindependencyofthephotoperiod AT santiagomorenojulian theinfluenceofwashingspanishibexcaprapyrenaicaspermontheeffectsofcryopreservationindependencyofthephotoperiod AT colomama influenceofwashingspanishibexcaprapyrenaicaspermontheeffectsofcryopreservationindependencyofthephotoperiod AT toledanodiazadolfo influenceofwashingspanishibexcaprapyrenaicaspermontheeffectsofcryopreservationindependencyofthephotoperiod AT lopezsebastianantonio influenceofwashingspanishibexcaprapyrenaicaspermontheeffectsofcryopreservationindependencyofthephotoperiod AT santiagomorenojulian influenceofwashingspanishibexcaprapyrenaicaspermontheeffectsofcryopreservationindependencyofthephotoperiod |
_version_ |
1767603254606168064 |