SSR markers for monitoring an in vitro core collection of Populus tremula
Core collections efficiently preserve genetic diversity and facilitate collection management and "ex situ" conservation of genetic resources. Populus tremula (European aspen) is demanded by plant growers and breeders but there are very few data on the genetic variation in the geographic distribution of this species in Spain. Microsatellite markers are useful for the evaluation of genetic variability and identification due to their abundance and polymorphism. Six pairs of oligonucleotide primers designed for simple sequence repeats (SSR) of quaking aspen (P. tremuloides) were successfully used in European aspen. Number of alleles per locus were low in this species in comparison to outcrossed, long-lived woody perennials due to the importance of vegetative propagation by root suckers in aspen stands. Mean heterozygosity (0.475) was similar to that reported in quaking aspen. Cluster analysis suggests that postglacial migration of European aspen took place from the Pyrenees in two directions westward and southward through mountain ranges of the Iberian Peninsula. To preserve the allelic richness of the P. tremula in vitro collection in Spain, a core collection of 26 clones is proposed.
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Format: | artículo biblioteca |
Language: | English |
Published: |
J.D. Sauerländer's Verlag
2003
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Subjects: | Allelic richness, Core collection, Genetic resources, Microsatellites, Populus tremula, |
Online Access: | http://hdl.handle.net/20.500.12792/1755 http://hdl.handle.net/10261/291672 |
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dig-inia-es-10261-2916722023-02-20T07:20:48Z SSR markers for monitoring an in vitro core collection of Populus tremula Gómez, A. Manzanera, J. A. Aguiriano Labandibar, Miren Edurne Grau, J. M. Bueno, M. A. Allelic richness Core collection Genetic resources Microsatellites Populus tremula Core collections efficiently preserve genetic diversity and facilitate collection management and "ex situ" conservation of genetic resources. Populus tremula (European aspen) is demanded by plant growers and breeders but there are very few data on the genetic variation in the geographic distribution of this species in Spain. Microsatellite markers are useful for the evaluation of genetic variability and identification due to their abundance and polymorphism. Six pairs of oligonucleotide primers designed for simple sequence repeats (SSR) of quaking aspen (P. tremuloides) were successfully used in European aspen. Number of alleles per locus were low in this species in comparison to outcrossed, long-lived woody perennials due to the importance of vegetative propagation by root suckers in aspen stands. Mean heterozygosity (0.475) was similar to that reported in quaking aspen. Cluster analysis suggests that postglacial migration of European aspen took place from the Pyrenees in two directions westward and southward through mountain ranges of the Iberian Peninsula. To preserve the allelic richness of the P. tremula in vitro collection in Spain, a core collection of 26 clones is proposed. 2023-02-20T07:20:48Z 2023-02-20T07:20:48Z 2003 artículo Silvae Genetica 52: 5-6 (2003) 0037-5349 http://hdl.handle.net/20.500.12792/1755 http://hdl.handle.net/10261/291672 en none J.D. Sauerländer's Verlag |
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Allelic richness Core collection Genetic resources Microsatellites Populus tremula Allelic richness Core collection Genetic resources Microsatellites Populus tremula |
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Allelic richness Core collection Genetic resources Microsatellites Populus tremula Allelic richness Core collection Genetic resources Microsatellites Populus tremula Gómez, A. Manzanera, J. A. Aguiriano Labandibar, Miren Edurne Grau, J. M. Bueno, M. A. SSR markers for monitoring an in vitro core collection of Populus tremula |
description |
Core collections efficiently preserve genetic diversity and facilitate collection management and "ex situ" conservation of genetic resources. Populus tremula (European aspen) is demanded by plant growers and breeders but there are very few data on the genetic variation in the geographic distribution of this species in Spain. Microsatellite markers are useful for the evaluation of genetic variability and identification due to their abundance and polymorphism. Six pairs of oligonucleotide primers designed for simple sequence repeats (SSR) of quaking aspen (P. tremuloides) were successfully used in European aspen. Number of alleles per locus were low in this species in comparison to outcrossed, long-lived woody perennials due to the importance of vegetative propagation by root suckers in aspen stands. Mean heterozygosity (0.475) was similar to that reported in quaking aspen. Cluster analysis suggests that postglacial migration of European aspen took place from the Pyrenees in two directions westward and southward through mountain ranges of the Iberian Peninsula. To preserve the allelic richness of the P. tremula in vitro collection in Spain, a core collection of 26 clones is proposed. |
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artículo |
topic_facet |
Allelic richness Core collection Genetic resources Microsatellites Populus tremula |
author |
Gómez, A. Manzanera, J. A. Aguiriano Labandibar, Miren Edurne Grau, J. M. Bueno, M. A. |
author_facet |
Gómez, A. Manzanera, J. A. Aguiriano Labandibar, Miren Edurne Grau, J. M. Bueno, M. A. |
author_sort |
Gómez, A. |
title |
SSR markers for monitoring an in vitro core collection of Populus tremula |
title_short |
SSR markers for monitoring an in vitro core collection of Populus tremula |
title_full |
SSR markers for monitoring an in vitro core collection of Populus tremula |
title_fullStr |
SSR markers for monitoring an in vitro core collection of Populus tremula |
title_full_unstemmed |
SSR markers for monitoring an in vitro core collection of Populus tremula |
title_sort |
ssr markers for monitoring an in vitro core collection of populus tremula |
publisher |
J.D. Sauerländer's Verlag |
publishDate |
2003 |
url |
http://hdl.handle.net/20.500.12792/1755 http://hdl.handle.net/10261/291672 |
work_keys_str_mv |
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1767603239191052288 |