Cryoprotectant effects of egg yolk on Indian red jungle fowl (Gallus gallus murghi) sperm
Egg yolk is a good external cryoprotectant of mammalian sperm and some wild bird's sperm, but, at least in domestic breeds of chicken (Gallus gallus domesticus), it may inhibit eventual fertilization of ova when high concentrations are used. We hypothesized that egg yolk can protect the sperm from cryo-induced damages providing adequate fertilization in one phylogenetic wild ancestor of current chicken breeds: the Indian red jungle fowl (Gallus gallus murghi). To test the hypothesis, the present study was designed to evaluate different concentrations of egg yolk in extender in comparison with glycerol. Semen collected from Indian red jungle fowl cocks (n = 8) was cryopreserved using different egg yolk concentrations (10%, 15%, 20% and 25%) or 20% glycerol (control group) following routine protocol of cryopreservation. During cryopreservation, sperm motility (67.5 ± 2.5%), plasma membrane integrity (66.3 ± 2.4%), viability (58.8 ± 1.3%) and acrosomal integrity (60.0.8 ± 2.0%) were recorded highest in an extender with 15% egg yolk compared to other experimental extenders and control at post-dilution, cooling, equilibration and thawing. The no. of fertilized eggs (26.6 ± 0.7, 21.6 ± 1.2), percent fertility (55.9 ± 4.4, 46.5 ± 2.2), no. of hatched chicks (23.6 ± 1.0, 17.2 ± 1.0), percent hatch (49.5 ± 3.2, 37.1 ± 2.5%) and hatchability of the fertile eggs (89.4 ± 2.2, 79.7 ± 3.7) were recorded higher (P < 0.05) with semen cryopreserved with 15% egg yolk compared to control (20% glycerol). It is concluded that 15% egg yolk can be used in cryopreservation protocol of Indian red jungle fowl sperm.
Main Authors: | Rakha, B. A., Ansari, M. S., Akhter, S., Santiago Moreno, Julián, Blesbois, E. |
---|---|
Format: | artículo biblioteca |
Language: | English |
Published: |
Elsevier
2018
|
Subjects: | Cryo-damage, Motility, Fertility, Acrosome, Sperm viability, Rooster, |
Online Access: | http://hdl.handle.net/20.500.12792/723 http://hdl.handle.net/10261/291420 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Dimethyleacetamide improves the cryosurvivability of Indian red jungle fowl (Gallus gallus murghi) sperm
by: Rakha, B. A., et al.
Published: (2017) -
Effect of dimethylformamide on sperm quality and fertilizing ability of Indian red jungle fowl (Gallus gallus murghi)
by: Rakha, Bushra A., et al.
Published: (2020-06) -
Effect of glutathione on pre and post-freezing sperm quality of Indian red jungle fowl (Gallus gallus murghi)
by: Ansari, M. S., et al.
Published: (2021-09-15) -
Transit along the vas deferens results in a high percentage of filiform spermatozoa with compacted chromatin in the rooster (Gallus domesticus)
by: Bernal Juárez, Berenice, et al.
Published: (2022-04-04) -
Research Note: Evaluation of two methods for adding cryoprotectant to semen and effects of bovine serum albumin on quality characteristics of cryopreserved rooster spermatozoa
by: Behnamifar, Alireza, et al.
Published: (2021-06)