Cryoprotectant effects of egg yolk on Indian red jungle fowl (Gallus gallus murghi) sperm
Egg yolk is a good external cryoprotectant of mammalian sperm and some wild bird's sperm, but, at least in domestic breeds of chicken (Gallus gallus domesticus), it may inhibit eventual fertilization of ova when high concentrations are used. We hypothesized that egg yolk can protect the sperm from cryo-induced damages providing adequate fertilization in one phylogenetic wild ancestor of current chicken breeds: the Indian red jungle fowl (Gallus gallus murghi). To test the hypothesis, the present study was designed to evaluate different concentrations of egg yolk in extender in comparison with glycerol. Semen collected from Indian red jungle fowl cocks (n = 8) was cryopreserved using different egg yolk concentrations (10%, 15%, 20% and 25%) or 20% glycerol (control group) following routine protocol of cryopreservation. During cryopreservation, sperm motility (67.5 ± 2.5%), plasma membrane integrity (66.3 ± 2.4%), viability (58.8 ± 1.3%) and acrosomal integrity (60.0.8 ± 2.0%) were recorded highest in an extender with 15% egg yolk compared to other experimental extenders and control at post-dilution, cooling, equilibration and thawing. The no. of fertilized eggs (26.6 ± 0.7, 21.6 ± 1.2), percent fertility (55.9 ± 4.4, 46.5 ± 2.2), no. of hatched chicks (23.6 ± 1.0, 17.2 ± 1.0), percent hatch (49.5 ± 3.2, 37.1 ± 2.5%) and hatchability of the fertile eggs (89.4 ± 2.2, 79.7 ± 3.7) were recorded higher (P < 0.05) with semen cryopreserved with 15% egg yolk compared to control (20% glycerol). It is concluded that 15% egg yolk can be used in cryopreservation protocol of Indian red jungle fowl sperm.
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Format: | artículo biblioteca |
Language: | English |
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Elsevier
2018
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Subjects: | Cryo-damage, Motility, Fertility, Acrosome, Sperm viability, Rooster, |
Online Access: | http://hdl.handle.net/20.500.12792/723 http://hdl.handle.net/10261/291420 |
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dig-inia-es-10261-2914202023-02-20T07:18:01Z Cryoprotectant effects of egg yolk on Indian red jungle fowl (Gallus gallus murghi) sperm Rakha, B. A. Ansari, M. S. Akhter, S. Santiago Moreno, Julián Blesbois, E. Cryo-damage Motility Fertility Acrosome Sperm viability Rooster Egg yolk is a good external cryoprotectant of mammalian sperm and some wild bird's sperm, but, at least in domestic breeds of chicken (Gallus gallus domesticus), it may inhibit eventual fertilization of ova when high concentrations are used. We hypothesized that egg yolk can protect the sperm from cryo-induced damages providing adequate fertilization in one phylogenetic wild ancestor of current chicken breeds: the Indian red jungle fowl (Gallus gallus murghi). To test the hypothesis, the present study was designed to evaluate different concentrations of egg yolk in extender in comparison with glycerol. Semen collected from Indian red jungle fowl cocks (n = 8) was cryopreserved using different egg yolk concentrations (10%, 15%, 20% and 25%) or 20% glycerol (control group) following routine protocol of cryopreservation. During cryopreservation, sperm motility (67.5 ± 2.5%), plasma membrane integrity (66.3 ± 2.4%), viability (58.8 ± 1.3%) and acrosomal integrity (60.0.8 ± 2.0%) were recorded highest in an extender with 15% egg yolk compared to other experimental extenders and control at post-dilution, cooling, equilibration and thawing. The no. of fertilized eggs (26.6 ± 0.7, 21.6 ± 1.2), percent fertility (55.9 ± 4.4, 46.5 ± 2.2), no. of hatched chicks (23.6 ± 1.0, 17.2 ± 1.0), percent hatch (49.5 ± 3.2, 37.1 ± 2.5%) and hatchability of the fertile eggs (89.4 ± 2.2, 79.7 ± 3.7) were recorded higher (P < 0.05) with semen cryopreserved with 15% egg yolk compared to control (20% glycerol). It is concluded that 15% egg yolk can be used in cryopreservation protocol of Indian red jungle fowl sperm. 2023-02-20T07:18:01Z 2023-02-20T07:18:01Z 2018 artículo Theriogenology 119: 150-155 (2018) 0093-691X http://hdl.handle.net/20.500.12792/723 http://hdl.handle.net/10261/291420 10.1016/j.theriogenology.2018.06.015 1879-3231 en none Elsevier |
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Cryo-damage Motility Fertility Acrosome Sperm viability Rooster Cryo-damage Motility Fertility Acrosome Sperm viability Rooster |
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Cryo-damage Motility Fertility Acrosome Sperm viability Rooster Cryo-damage Motility Fertility Acrosome Sperm viability Rooster Rakha, B. A. Ansari, M. S. Akhter, S. Santiago Moreno, Julián Blesbois, E. Cryoprotectant effects of egg yolk on Indian red jungle fowl (Gallus gallus murghi) sperm |
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Egg yolk is a good external cryoprotectant of mammalian sperm and some wild bird's sperm, but, at least in domestic breeds of chicken (Gallus gallus domesticus), it may inhibit eventual fertilization of ova when high concentrations are used. We hypothesized that egg yolk can protect the sperm from cryo-induced damages providing adequate fertilization in one phylogenetic wild ancestor of current chicken breeds: the Indian red jungle fowl (Gallus gallus murghi). To test the hypothesis, the present study was designed to evaluate different concentrations of egg yolk in extender in comparison with glycerol. Semen collected from Indian red jungle fowl cocks (n = 8) was cryopreserved using different egg yolk concentrations (10%, 15%, 20% and 25%) or 20% glycerol (control group) following routine protocol of cryopreservation. During cryopreservation, sperm motility (67.5 ± 2.5%), plasma membrane integrity (66.3 ± 2.4%), viability (58.8 ± 1.3%) and acrosomal integrity (60.0.8 ± 2.0%) were recorded highest in an extender with 15% egg yolk compared to other experimental extenders and control at post-dilution, cooling, equilibration and thawing. The no. of fertilized eggs (26.6 ± 0.7, 21.6 ± 1.2), percent fertility (55.9 ± 4.4, 46.5 ± 2.2), no. of hatched chicks (23.6 ± 1.0, 17.2 ± 1.0), percent hatch (49.5 ± 3.2, 37.1 ± 2.5%) and hatchability of the fertile eggs (89.4 ± 2.2, 79.7 ± 3.7) were recorded higher (P < 0.05) with semen cryopreserved with 15% egg yolk compared to control (20% glycerol). It is concluded that 15% egg yolk can be used in cryopreservation protocol of Indian red jungle fowl sperm. |
format |
artículo |
topic_facet |
Cryo-damage Motility Fertility Acrosome Sperm viability Rooster |
author |
Rakha, B. A. Ansari, M. S. Akhter, S. Santiago Moreno, Julián Blesbois, E. |
author_facet |
Rakha, B. A. Ansari, M. S. Akhter, S. Santiago Moreno, Julián Blesbois, E. |
author_sort |
Rakha, B. A. |
title |
Cryoprotectant effects of egg yolk on Indian red jungle fowl (Gallus gallus murghi) sperm |
title_short |
Cryoprotectant effects of egg yolk on Indian red jungle fowl (Gallus gallus murghi) sperm |
title_full |
Cryoprotectant effects of egg yolk on Indian red jungle fowl (Gallus gallus murghi) sperm |
title_fullStr |
Cryoprotectant effects of egg yolk on Indian red jungle fowl (Gallus gallus murghi) sperm |
title_full_unstemmed |
Cryoprotectant effects of egg yolk on Indian red jungle fowl (Gallus gallus murghi) sperm |
title_sort |
cryoprotectant effects of egg yolk on indian red jungle fowl (gallus gallus murghi) sperm |
publisher |
Elsevier |
publishDate |
2018 |
url |
http://hdl.handle.net/20.500.12792/723 http://hdl.handle.net/10261/291420 |
work_keys_str_mv |
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