The proximal promoter region of mTert is sufficient to regulate telomerase activity in ES cells and transgenic animals
Background The reverse transcriptase of telomerase (Tert) controls telomerase activity maintaining the end of linear chromosomes in eukaryotic cells. Telomerase function is highly active in undifferentiated multipotent stem cells, decreases with cell differentiation and is generally absent from most somatic cells in the adult. Its absence is responsible of telomeres shortening in such somatic cells. Using an in vivo transgenic model and an in vitro culture differentiation of adult stem cells, we examined the elements of the mouse Tert (mTert) promoter that control telomerase activity. Results Three constructs comprising 1, 2 or 5 kb of the mTert promoter sequence coupled to the coding sequence of the green fluorescent protein (EGFP) were electroporated into embryonic stem (ES) cells. Transformed ES cells were able to mimic the expected mTert expression, which was associated to green fluorescence. One and 5 kb promoter produced the higher expression of EGFP, on ES cells. When ES cells were allowed to differentiate to embryoid bodies and to other cell types, they lost gradually the expression of mTert-EGFP as consequence of differentiation. No differences were found among the three constructs analyzed. We then generated transgenic mice with the three constructs. Expression of the reporter gene was monitored by reverse transcription-PCR analysis and EGFP visualization. The mRNA expression of the three constructs was lower than the endogenous mTert, but mimicked the endogenous mTert transcription pattern; however, no fluorescent expression of EGFP was detected in adult tissues. EGFP expression of the three constructs was visualized at the blastocysts stage and in new ES cells generated from them; in the germinal ring of E13 dpc foetuses; in ES-like colonies and in germinal stem cells generated from neonatal and adult testis cells; and in neuroesferes generated from E14 dpc foetuses' brain cells. Conclusion The 1 kb promoter upstream of the initiating ATG codon of mTert contains all the regulatory elements to control telomerase expression in ES cells during in vitro loss of pluripotency. The transgenic mouse lines generated represent an appropriate system to analyze the expression of mouse Tert gene under physiological condition and during establishment of stem cell lines generated from embryonic or adult tissues.
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BioMed Central
2006
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dig-inia-es-10261-2914072023-02-20T07:17:53Z The proximal promoter region of mTert is sufficient to regulate telomerase activity in ES cells and transgenic animals Pericuesta Camacho, Eva Ramírez De Paz, Miguel Ángel Villa Díaz, Ana Relaño-Gines, A. Torres, J. M. Nieto, M. Pintado, B. Gutiérrez Adán, Alfonso Background The reverse transcriptase of telomerase (Tert) controls telomerase activity maintaining the end of linear chromosomes in eukaryotic cells. Telomerase function is highly active in undifferentiated multipotent stem cells, decreases with cell differentiation and is generally absent from most somatic cells in the adult. Its absence is responsible of telomeres shortening in such somatic cells. Using an in vivo transgenic model and an in vitro culture differentiation of adult stem cells, we examined the elements of the mouse Tert (mTert) promoter that control telomerase activity. Results Three constructs comprising 1, 2 or 5 kb of the mTert promoter sequence coupled to the coding sequence of the green fluorescent protein (EGFP) were electroporated into embryonic stem (ES) cells. Transformed ES cells were able to mimic the expected mTert expression, which was associated to green fluorescence. One and 5 kb promoter produced the higher expression of EGFP, on ES cells. When ES cells were allowed to differentiate to embryoid bodies and to other cell types, they lost gradually the expression of mTert-EGFP as consequence of differentiation. No differences were found among the three constructs analyzed. We then generated transgenic mice with the three constructs. Expression of the reporter gene was monitored by reverse transcription-PCR analysis and EGFP visualization. The mRNA expression of the three constructs was lower than the endogenous mTert, but mimicked the endogenous mTert transcription pattern; however, no fluorescent expression of EGFP was detected in adult tissues. EGFP expression of the three constructs was visualized at the blastocysts stage and in new ES cells generated from them; in the germinal ring of E13 dpc foetuses; in ES-like colonies and in germinal stem cells generated from neonatal and adult testis cells; and in neuroesferes generated from E14 dpc foetuses' brain cells. Conclusion The 1 kb promoter upstream of the initiating ATG codon of mTert contains all the regulatory elements to control telomerase expression in ES cells during in vitro loss of pluripotency. The transgenic mouse lines generated represent an appropriate system to analyze the expression of mouse Tert gene under physiological condition and during establishment of stem cell lines generated from embryonic or adult tissues. 2023-02-20T07:17:53Z 2023-02-20T07:17:53Z 2006 artículo Reproductive Biology and Endocrinology 4: e5 (2006) http://hdl.handle.net/20.500.12792/5392 http://hdl.handle.net/10261/291407 10.1186/1477-7827-4-5 1477-7827 en open BioMed Central |
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Background The reverse transcriptase of telomerase (Tert) controls telomerase activity maintaining the end of linear chromosomes in eukaryotic cells. Telomerase function is highly active in undifferentiated multipotent stem cells, decreases with cell differentiation and is generally absent from most somatic cells in the adult. Its absence is responsible of telomeres shortening in such somatic cells. Using an in vivo transgenic model and an in vitro culture differentiation of adult stem cells, we examined the elements of the mouse Tert (mTert) promoter that control telomerase activity. Results Three constructs comprising 1, 2 or 5 kb of the mTert promoter sequence coupled to the coding sequence of the green fluorescent protein (EGFP) were electroporated into embryonic stem (ES) cells. Transformed ES cells were able to mimic the expected mTert expression, which was associated to green fluorescence. One and 5 kb promoter produced the higher expression of EGFP, on ES cells. When ES cells were allowed to differentiate to embryoid bodies and to other cell types, they lost gradually the expression of mTert-EGFP as consequence of differentiation. No differences were found among the three constructs analyzed. We then generated transgenic mice with the three constructs. Expression of the reporter gene was monitored by reverse transcription-PCR analysis and EGFP visualization. The mRNA expression of the three constructs was lower than the endogenous mTert, but mimicked the endogenous mTert transcription pattern; however, no fluorescent expression of EGFP was detected in adult tissues. EGFP expression of the three constructs was visualized at the blastocysts stage and in new ES cells generated from them; in the germinal ring of E13 dpc foetuses; in ES-like colonies and in germinal stem cells generated from neonatal and adult testis cells; and in neuroesferes generated from E14 dpc foetuses' brain cells. Conclusion The 1 kb promoter upstream of the initiating ATG codon of mTert contains all the regulatory elements to control telomerase expression in ES cells during in vitro loss of pluripotency. The transgenic mouse lines generated represent an appropriate system to analyze the expression of mouse Tert gene under physiological condition and during establishment of stem cell lines generated from embryonic or adult tissues. |
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Pericuesta Camacho, Eva Ramírez De Paz, Miguel Ángel Villa Díaz, Ana Relaño-Gines, A. Torres, J. M. Nieto, M. Pintado, B. Gutiérrez Adán, Alfonso |
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Pericuesta Camacho, Eva Ramírez De Paz, Miguel Ángel Villa Díaz, Ana Relaño-Gines, A. Torres, J. M. Nieto, M. Pintado, B. Gutiérrez Adán, Alfonso The proximal promoter region of mTert is sufficient to regulate telomerase activity in ES cells and transgenic animals |
author_facet |
Pericuesta Camacho, Eva Ramírez De Paz, Miguel Ángel Villa Díaz, Ana Relaño-Gines, A. Torres, J. M. Nieto, M. Pintado, B. Gutiérrez Adán, Alfonso |
author_sort |
Pericuesta Camacho, Eva |
title |
The proximal promoter region of mTert is sufficient to regulate telomerase activity in ES cells and transgenic animals |
title_short |
The proximal promoter region of mTert is sufficient to regulate telomerase activity in ES cells and transgenic animals |
title_full |
The proximal promoter region of mTert is sufficient to regulate telomerase activity in ES cells and transgenic animals |
title_fullStr |
The proximal promoter region of mTert is sufficient to regulate telomerase activity in ES cells and transgenic animals |
title_full_unstemmed |
The proximal promoter region of mTert is sufficient to regulate telomerase activity in ES cells and transgenic animals |
title_sort |
proximal promoter region of mtert is sufficient to regulate telomerase activity in es cells and transgenic animals |
publisher |
BioMed Central |
publishDate |
2006 |
url |
http://hdl.handle.net/20.500.12792/5392 http://hdl.handle.net/10261/291407 |
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