Late stage cathepsin C, CXCL13 and Ki-67 overexpression correlate with regional neuropathology in a BSE transgenic murine model
A DNA microarray-based gene expression analysis study was performed with bovine spongiform encephalopathy (BSE) transgenic mice. Several genes were found to be overexpressed including the lysosomal enzyme cathepsin C, the chemokine CXCL13 and a number of genes related to cellular proliferation. The brains from terminal stage, BSE inoculated, 'bovinized', transgenic mice were subjected to immunohistochemistry with antibodies against these two proteins and Ki-67, a cell proliferation marker, to assess the biological relevance of the gene expression changes. Differential expression of cathepsin C and CXCL13 proteins and increased expression of Ki-67 was observed. These changes were localized to areas of deposition of PrPres and spongiform change and to areas showing an astroglial and microglial response. These findings suggest that these proteins are involved in the mechanisms leading to the establishment of transmissible spongiform encephalopathy. © 2012 Elsevier Ltd.
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Elsevier
2013
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Subjects: | BSE, Cathepsin C, CXCL13, Ki-67, |
Online Access: | http://hdl.handle.net/20.500.12792/4691 http://hdl.handle.net/10261/291288 |
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dig-inia-es-10261-2912882023-02-20T07:16:37Z Late stage cathepsin C, CXCL13 and Ki-67 overexpression correlate with regional neuropathology in a BSE transgenic murine model Vidal, E. Tortosa, R. Marco, P. Fondevila, D. Rabanal, R. M. Torres, J. M. Pumarola, M. BSE Cathepsin C CXCL13 Ki-67 A DNA microarray-based gene expression analysis study was performed with bovine spongiform encephalopathy (BSE) transgenic mice. Several genes were found to be overexpressed including the lysosomal enzyme cathepsin C, the chemokine CXCL13 and a number of genes related to cellular proliferation. The brains from terminal stage, BSE inoculated, 'bovinized', transgenic mice were subjected to immunohistochemistry with antibodies against these two proteins and Ki-67, a cell proliferation marker, to assess the biological relevance of the gene expression changes. Differential expression of cathepsin C and CXCL13 proteins and increased expression of Ki-67 was observed. These changes were localized to areas of deposition of PrPres and spongiform change and to areas showing an astroglial and microglial response. These findings suggest that these proteins are involved in the mechanisms leading to the establishment of transmissible spongiform encephalopathy. © 2012 Elsevier Ltd. 2023-02-20T07:16:37Z 2023-02-20T07:16:37Z 2013 artículo Journal of Comparative Pathology 148: 22-32 (2013) 0021-9975 http://hdl.handle.net/20.500.12792/4691 http://hdl.handle.net/10261/291288 10.1016/j.jcpa.2012.05.004 1532-3129 en none Elsevier |
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BSE Cathepsin C CXCL13 Ki-67 BSE Cathepsin C CXCL13 Ki-67 Vidal, E. Tortosa, R. Marco, P. Fondevila, D. Rabanal, R. M. Torres, J. M. Pumarola, M. Late stage cathepsin C, CXCL13 and Ki-67 overexpression correlate with regional neuropathology in a BSE transgenic murine model |
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A DNA microarray-based gene expression analysis study was performed with bovine spongiform encephalopathy (BSE) transgenic mice. Several genes were found to be overexpressed including the lysosomal enzyme cathepsin C, the chemokine CXCL13 and a number of genes related to cellular proliferation. The brains from terminal stage, BSE inoculated, 'bovinized', transgenic mice were subjected to immunohistochemistry with antibodies against these two proteins and Ki-67, a cell proliferation marker, to assess the biological relevance of the gene expression changes. Differential expression of cathepsin C and CXCL13 proteins and increased expression of Ki-67 was observed. These changes were localized to areas of deposition of PrPres and spongiform change and to areas showing an astroglial and microglial response. These findings suggest that these proteins are involved in the mechanisms leading to the establishment of transmissible spongiform encephalopathy. © 2012 Elsevier Ltd. |
format |
artículo |
topic_facet |
BSE Cathepsin C CXCL13 Ki-67 |
author |
Vidal, E. Tortosa, R. Marco, P. Fondevila, D. Rabanal, R. M. Torres, J. M. Pumarola, M. |
author_facet |
Vidal, E. Tortosa, R. Marco, P. Fondevila, D. Rabanal, R. M. Torres, J. M. Pumarola, M. |
author_sort |
Vidal, E. |
title |
Late stage cathepsin C, CXCL13 and Ki-67 overexpression correlate with regional neuropathology in a BSE transgenic murine model |
title_short |
Late stage cathepsin C, CXCL13 and Ki-67 overexpression correlate with regional neuropathology in a BSE transgenic murine model |
title_full |
Late stage cathepsin C, CXCL13 and Ki-67 overexpression correlate with regional neuropathology in a BSE transgenic murine model |
title_fullStr |
Late stage cathepsin C, CXCL13 and Ki-67 overexpression correlate with regional neuropathology in a BSE transgenic murine model |
title_full_unstemmed |
Late stage cathepsin C, CXCL13 and Ki-67 overexpression correlate with regional neuropathology in a BSE transgenic murine model |
title_sort |
late stage cathepsin c, cxcl13 and ki-67 overexpression correlate with regional neuropathology in a bse transgenic murine model |
publisher |
Elsevier |
publishDate |
2013 |
url |
http://hdl.handle.net/20.500.12792/4691 http://hdl.handle.net/10261/291288 |
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