Molecularly imprinted polymer-hollow fiber microextraction of hydrophilic fluoroquinolone antibiotics in environmental waters and urine samples
In the present work, the preparation of a new selective molecularly imprinted polymer (MIP) for the family of fluoroquinolones (FQs) in the pores of polypropylene hollow fibers (HFs) is proposed. The resulting MIP-HFs, which combine solid-phase microextraction (SPME) and molecular imprinting technologies, were used to develop a selective microextraction methodology (MIP-HFM) to determine selected FQs danofloxacin, norfloxacin, enrofloxacin and ciprofloxacin in real samples of environmental and biological interest. Measurements during the optimization of the MIP-HFM and its application to the analyses of real samples were performed by HPLC-UV and HPLC-MS/MS. In order to establish optimum rebinding conditions, the effect of key experimental parameters such as loading media, extraction time and stirring-rate were studied. The developed MIP composites exhibited recognition properties towards the selected hydrophilic antibiotics in non-polar media (toluene) and in polar protic systems such as methanol and methanol/water solutions, up to 20% water content. Recoveries by the developed method for all FQs tested in surface water, groundwater and urine spiked with the analytes of interest at two different concentration levels were within 9.4-24.5 %, with a relative standard deviation, generally <20% (n = 3). The detection limits were within 0.1-10 μg L-1, depending upon the antibiotic and the type of sample.
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Format: | artículo biblioteca |
Language: | English |
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Elsevier
2019
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Subjects: | Aqueous samples, Fluoroquinolones, MIP hollow fiber, Microextraction, Miniaturized extraction techniques, Molecularly imprinted polymers, |
Online Access: | http://hdl.handle.net/20.500.12792/755 http://hdl.handle.net/10261/291150 |
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dig-inia-es-10261-2911502023-02-20T07:15:00Z Molecularly imprinted polymer-hollow fiber microextraction of hydrophilic fluoroquinolone antibiotics in environmental waters and urine samples Barahona, F. Albero Romano, María Beatriz Tadeo Lluch, José Luis Martín Esteban, Antonio Aqueous samples Fluoroquinolones MIP hollow fiber Microextraction Miniaturized extraction techniques Molecularly imprinted polymers In the present work, the preparation of a new selective molecularly imprinted polymer (MIP) for the family of fluoroquinolones (FQs) in the pores of polypropylene hollow fibers (HFs) is proposed. The resulting MIP-HFs, which combine solid-phase microextraction (SPME) and molecular imprinting technologies, were used to develop a selective microextraction methodology (MIP-HFM) to determine selected FQs danofloxacin, norfloxacin, enrofloxacin and ciprofloxacin in real samples of environmental and biological interest. Measurements during the optimization of the MIP-HFM and its application to the analyses of real samples were performed by HPLC-UV and HPLC-MS/MS. In order to establish optimum rebinding conditions, the effect of key experimental parameters such as loading media, extraction time and stirring-rate were studied. The developed MIP composites exhibited recognition properties towards the selected hydrophilic antibiotics in non-polar media (toluene) and in polar protic systems such as methanol and methanol/water solutions, up to 20% water content. Recoveries by the developed method for all FQs tested in surface water, groundwater and urine spiked with the analytes of interest at two different concentration levels were within 9.4-24.5 %, with a relative standard deviation, generally <20% (n = 3). The detection limits were within 0.1-10 μg L-1, depending upon the antibiotic and the type of sample. 2023-02-20T07:15:00Z 2023-02-20T07:15:00Z 2019 artículo Journal of Chromatography A 1587: 42-49 (2019) 0021-9673 http://hdl.handle.net/20.500.12792/755 http://hdl.handle.net/10261/291150 10.1016/j.chroma.2018.12.015 1873-3778 en none Elsevier |
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Aqueous samples Fluoroquinolones MIP hollow fiber Microextraction Miniaturized extraction techniques Molecularly imprinted polymers Aqueous samples Fluoroquinolones MIP hollow fiber Microextraction Miniaturized extraction techniques Molecularly imprinted polymers |
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Aqueous samples Fluoroquinolones MIP hollow fiber Microextraction Miniaturized extraction techniques Molecularly imprinted polymers Aqueous samples Fluoroquinolones MIP hollow fiber Microextraction Miniaturized extraction techniques Molecularly imprinted polymers Barahona, F. Albero Romano, María Beatriz Tadeo Lluch, José Luis Martín Esteban, Antonio Molecularly imprinted polymer-hollow fiber microextraction of hydrophilic fluoroquinolone antibiotics in environmental waters and urine samples |
description |
In the present work, the preparation of a new selective molecularly imprinted polymer (MIP) for the family of fluoroquinolones (FQs) in the pores of polypropylene hollow fibers (HFs) is proposed. The resulting MIP-HFs, which combine solid-phase microextraction (SPME) and molecular imprinting technologies, were used to develop a selective microextraction methodology (MIP-HFM) to determine selected FQs danofloxacin, norfloxacin, enrofloxacin and ciprofloxacin in real samples of environmental and biological interest. Measurements during the optimization of the MIP-HFM and its application to the analyses of real samples were performed by HPLC-UV and HPLC-MS/MS. In order to establish optimum rebinding conditions, the effect of key experimental parameters such as loading media, extraction time and stirring-rate were studied. The developed MIP composites exhibited recognition properties towards the selected hydrophilic antibiotics in non-polar media (toluene) and in polar protic systems such as methanol and methanol/water solutions, up to 20% water content. Recoveries by the developed method for all FQs tested in surface water, groundwater and urine spiked with the analytes of interest at two different concentration levels were within 9.4-24.5 %, with a relative standard deviation, generally <20% (n = 3). The detection limits were within 0.1-10 μg L-1, depending upon the antibiotic and the type of sample. |
format |
artículo |
topic_facet |
Aqueous samples Fluoroquinolones MIP hollow fiber Microextraction Miniaturized extraction techniques Molecularly imprinted polymers |
author |
Barahona, F. Albero Romano, María Beatriz Tadeo Lluch, José Luis Martín Esteban, Antonio |
author_facet |
Barahona, F. Albero Romano, María Beatriz Tadeo Lluch, José Luis Martín Esteban, Antonio |
author_sort |
Barahona, F. |
title |
Molecularly imprinted polymer-hollow fiber microextraction of hydrophilic fluoroquinolone antibiotics in environmental waters and urine samples |
title_short |
Molecularly imprinted polymer-hollow fiber microextraction of hydrophilic fluoroquinolone antibiotics in environmental waters and urine samples |
title_full |
Molecularly imprinted polymer-hollow fiber microextraction of hydrophilic fluoroquinolone antibiotics in environmental waters and urine samples |
title_fullStr |
Molecularly imprinted polymer-hollow fiber microextraction of hydrophilic fluoroquinolone antibiotics in environmental waters and urine samples |
title_full_unstemmed |
Molecularly imprinted polymer-hollow fiber microextraction of hydrophilic fluoroquinolone antibiotics in environmental waters and urine samples |
title_sort |
molecularly imprinted polymer-hollow fiber microextraction of hydrophilic fluoroquinolone antibiotics in environmental waters and urine samples |
publisher |
Elsevier |
publishDate |
2019 |
url |
http://hdl.handle.net/20.500.12792/755 http://hdl.handle.net/10261/291150 |
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