Molecular identification and microscopic characterization of poxvirus in a Guiana dolphin and a common bottlenose dolphin, Brazil
The poxviruses identified in cetaceans are associated with characteristic tattoo or ring skin lesions. However, little is known regarding the prevalence and progression of these lesions and the molecular characterization of cetacean poxviruses in the Southern Hemisphere. This manuscript describes the progression of poxvirus-like skin lesions in 5 free-ranging Guiana dolphins Sotalia guianensis. Additionally, 151 skin samples from 113 free-ranging cetaceans from Brazil, including 4 animals with tattoo skin lesions, were selected for poxvirus testing. Poxviral DNA polymerase gene PCR amplification was used to detect the virus in β-actin-positive samples (145/151). DNA topoisomerase I gene PCR was then used in Cetaceanpoxvirus (CePV)-positive cases (n = 2), which were further evaluated by histopathology and electron microscopy. Based on photo-identification, adult Guiana dolphins presented regressing or healed poxvirus-like lesions (2/2), while juveniles presented persistent (2/3) or healed and progressive lesions (1/3). CePV DNA was amplified in a common bottlenose dolphin Tursiops truncatus and in a Guiana dolphin. Intracytoplasmic inclusion bodies and viral particles consistent with poxvirus were identified by histology and electron microscopy, respectively. CePV-specific amino acid motifs were identified through phylogenetic analysis. Our findings corroborate previous studies that suggest the placement of poxviruses from cetaceans within the novel CePV genus. This is the first molecular identification of poxvirus in South American odontocetes.
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Format: | artículo biblioteca |
Language: | English |
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Inter Research
2018
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Subjects: | Cetacean poxvirus, Tattoo lesion, Pathology, Virology, Sotalia guianensis, Tursiops truncatus, South America, |
Online Access: | http://hdl.handle.net/20.500.12792/758 http://hdl.handle.net/10261/291149 |
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dig-inia-es-10261-2911492023-02-20T07:15:00Z Molecular identification and microscopic characterization of poxvirus in a Guiana dolphin and a common bottlenose dolphin, Brazil Sacristán, C. Esperón Fajardo, Fernando Marigo, J. Ewbank, A. C. De Carvalho, R. R. Groch, K. R. De Castilho, P. V. Sánchez-Sarmiento, A. M. Costa-Silva, S. Ferreira-Machado, E. Gonzales-Viera, O. A. Daura-Jorge, F. G. Santos-Neto, E. B. Lailson-Brito, J. De Freitas Azevedo, A. Simões-Lopes, P. C. Das Neves, C. G. Catão-Dias, J. L. Cetacean poxvirus Tattoo lesion Pathology Virology Sotalia guianensis Tursiops truncatus South America The poxviruses identified in cetaceans are associated with characteristic tattoo or ring skin lesions. However, little is known regarding the prevalence and progression of these lesions and the molecular characterization of cetacean poxviruses in the Southern Hemisphere. This manuscript describes the progression of poxvirus-like skin lesions in 5 free-ranging Guiana dolphins Sotalia guianensis. Additionally, 151 skin samples from 113 free-ranging cetaceans from Brazil, including 4 animals with tattoo skin lesions, were selected for poxvirus testing. Poxviral DNA polymerase gene PCR amplification was used to detect the virus in β-actin-positive samples (145/151). DNA topoisomerase I gene PCR was then used in Cetaceanpoxvirus (CePV)-positive cases (n = 2), which were further evaluated by histopathology and electron microscopy. Based on photo-identification, adult Guiana dolphins presented regressing or healed poxvirus-like lesions (2/2), while juveniles presented persistent (2/3) or healed and progressive lesions (1/3). CePV DNA was amplified in a common bottlenose dolphin Tursiops truncatus and in a Guiana dolphin. Intracytoplasmic inclusion bodies and viral particles consistent with poxvirus were identified by histology and electron microscopy, respectively. CePV-specific amino acid motifs were identified through phylogenetic analysis. Our findings corroborate previous studies that suggest the placement of poxviruses from cetaceans within the novel CePV genus. This is the first molecular identification of poxvirus in South American odontocetes. 2023-02-20T07:15:00Z 2023-02-20T07:15:00Z 2018 artículo Diseases of Aquatic Organisms 130(3): 177-185 (2018) 0177-5103 http://hdl.handle.net/20.500.12792/758 http://hdl.handle.net/10261/291149 10.3354/dao03271 1616-1580 en open Inter Research |
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Cetacean poxvirus Tattoo lesion Pathology Virology Sotalia guianensis Tursiops truncatus South America Cetacean poxvirus Tattoo lesion Pathology Virology Sotalia guianensis Tursiops truncatus South America |
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Cetacean poxvirus Tattoo lesion Pathology Virology Sotalia guianensis Tursiops truncatus South America Cetacean poxvirus Tattoo lesion Pathology Virology Sotalia guianensis Tursiops truncatus South America Sacristán, C. Esperón Fajardo, Fernando Marigo, J. Ewbank, A. C. De Carvalho, R. R. Groch, K. R. De Castilho, P. V. Sánchez-Sarmiento, A. M. Costa-Silva, S. Ferreira-Machado, E. Gonzales-Viera, O. A. Daura-Jorge, F. G. Santos-Neto, E. B. Lailson-Brito, J. De Freitas Azevedo, A. Simões-Lopes, P. C. Das Neves, C. G. Catão-Dias, J. L. Molecular identification and microscopic characterization of poxvirus in a Guiana dolphin and a common bottlenose dolphin, Brazil |
description |
The poxviruses identified in cetaceans are associated with characteristic tattoo or ring skin lesions. However, little is known regarding the prevalence and progression of these lesions and the molecular characterization of cetacean poxviruses in the Southern Hemisphere. This manuscript describes the progression of poxvirus-like skin lesions in 5 free-ranging Guiana dolphins Sotalia guianensis. Additionally, 151 skin samples from 113 free-ranging cetaceans from Brazil, including 4 animals with tattoo skin lesions, were selected for poxvirus testing. Poxviral DNA polymerase gene PCR amplification was used to detect the virus in β-actin-positive samples (145/151). DNA topoisomerase I gene PCR was then used in Cetaceanpoxvirus (CePV)-positive cases (n = 2), which were further evaluated by histopathology and electron microscopy. Based on photo-identification, adult Guiana dolphins presented regressing or healed poxvirus-like lesions (2/2), while juveniles presented persistent (2/3) or healed and progressive lesions (1/3). CePV DNA was amplified in a common bottlenose dolphin Tursiops truncatus and in a Guiana dolphin. Intracytoplasmic inclusion bodies and viral particles consistent with poxvirus were identified by histology and electron microscopy, respectively. CePV-specific amino acid motifs were identified through phylogenetic analysis. Our findings corroborate previous studies that suggest the placement of poxviruses from cetaceans within the novel CePV genus. This is the first molecular identification of poxvirus in South American odontocetes. |
format |
artículo |
topic_facet |
Cetacean poxvirus Tattoo lesion Pathology Virology Sotalia guianensis Tursiops truncatus South America |
author |
Sacristán, C. Esperón Fajardo, Fernando Marigo, J. Ewbank, A. C. De Carvalho, R. R. Groch, K. R. De Castilho, P. V. Sánchez-Sarmiento, A. M. Costa-Silva, S. Ferreira-Machado, E. Gonzales-Viera, O. A. Daura-Jorge, F. G. Santos-Neto, E. B. Lailson-Brito, J. De Freitas Azevedo, A. Simões-Lopes, P. C. Das Neves, C. G. Catão-Dias, J. L. |
author_facet |
Sacristán, C. Esperón Fajardo, Fernando Marigo, J. Ewbank, A. C. De Carvalho, R. R. Groch, K. R. De Castilho, P. V. Sánchez-Sarmiento, A. M. Costa-Silva, S. Ferreira-Machado, E. Gonzales-Viera, O. A. Daura-Jorge, F. G. Santos-Neto, E. B. Lailson-Brito, J. De Freitas Azevedo, A. Simões-Lopes, P. C. Das Neves, C. G. Catão-Dias, J. L. |
author_sort |
Sacristán, C. |
title |
Molecular identification and microscopic characterization of poxvirus in a Guiana dolphin and a common bottlenose dolphin, Brazil |
title_short |
Molecular identification and microscopic characterization of poxvirus in a Guiana dolphin and a common bottlenose dolphin, Brazil |
title_full |
Molecular identification and microscopic characterization of poxvirus in a Guiana dolphin and a common bottlenose dolphin, Brazil |
title_fullStr |
Molecular identification and microscopic characterization of poxvirus in a Guiana dolphin and a common bottlenose dolphin, Brazil |
title_full_unstemmed |
Molecular identification and microscopic characterization of poxvirus in a Guiana dolphin and a common bottlenose dolphin, Brazil |
title_sort |
molecular identification and microscopic characterization of poxvirus in a guiana dolphin and a common bottlenose dolphin, brazil |
publisher |
Inter Research |
publishDate |
2018 |
url |
http://hdl.handle.net/20.500.12792/758 http://hdl.handle.net/10261/291149 |
work_keys_str_mv |
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