Effect of bovine oviductal extracellular vesicles on embryo development and quality in vitro
The aim of this study was to evaluate the effect of extracellular vesicles (EV) from oviductal fluid (OF), either from the ampulla or isthmus, on the development and quality of in vitro-cultured bovine embryos. Zygotes were cultured in synthetic oviduct fluid (SOF+3mg/mL BSA) without calf serum (C− group), in the presence of 3×105 EV/mL from ampullary or isthmic OF at either 1×104g (10K) or 1×105 g (100K), and compared with SOF+5% FCS (C+ group). OF-EV size and concentration were assessed by electron microscopy and nanotracking analysis system. Embryo development was recorded on Days 7–9, and blastocyst quality was assessed through cryotolerance and gene expression analysis. Lower blastocyst yield was observed on Day 7 in the C− and OF-EV groups (12.0–14.3%) compared with C+ (20.6%); however, these differences were compensated at Days 8 and 9 (Day 9: 28.5–30.8%). Importantly, the survival rate of blastocysts produced with isthmic 100K OF-EV was higher than that of C+ and C− group at 72h after vitrification and warming (80.1 vs 34.5 and 50.5% respectively, P < 0.05). In terms of gene expression, blastocysts produced in the presence of 100 K isthmic OF-EV upregulated the water channel AQP3 and DNMT3A and SNRPN transcripts compared with the C+, with the expression in C- being intermediate. The lipid receptor LDLR was downregulated in C+ compared with all other groups. In conclusion, the addition of oviductal fluid extracellular vesicles from isthmus, to in vitro culture of bovine embryos in the absence of serum improves the development and quality of the embryos produced.
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BioScientifica
2017
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dig-inia-es-10261-2911392023-02-20T07:14:51Z Effect of bovine oviductal extracellular vesicles on embryo development and quality in vitro Lopera-Vasquez, R. Hamdi, M. Maillo, V. Gutiérrez Adán, Alfonso Bermejo Álvarez, Pablo Angel Ramírez, M. Yáñez-Mó, M. Rizos Dimitrios, Dimitrios The aim of this study was to evaluate the effect of extracellular vesicles (EV) from oviductal fluid (OF), either from the ampulla or isthmus, on the development and quality of in vitro-cultured bovine embryos. Zygotes were cultured in synthetic oviduct fluid (SOF+3mg/mL BSA) without calf serum (C− group), in the presence of 3×105 EV/mL from ampullary or isthmic OF at either 1×104g (10K) or 1×105 g (100K), and compared with SOF+5% FCS (C+ group). OF-EV size and concentration were assessed by electron microscopy and nanotracking analysis system. Embryo development was recorded on Days 7–9, and blastocyst quality was assessed through cryotolerance and gene expression analysis. Lower blastocyst yield was observed on Day 7 in the C− and OF-EV groups (12.0–14.3%) compared with C+ (20.6%); however, these differences were compensated at Days 8 and 9 (Day 9: 28.5–30.8%). Importantly, the survival rate of blastocysts produced with isthmic 100K OF-EV was higher than that of C+ and C− group at 72h after vitrification and warming (80.1 vs 34.5 and 50.5% respectively, P < 0.05). In terms of gene expression, blastocysts produced in the presence of 100 K isthmic OF-EV upregulated the water channel AQP3 and DNMT3A and SNRPN transcripts compared with the C+, with the expression in C- being intermediate. The lipid receptor LDLR was downregulated in C+ compared with all other groups. In conclusion, the addition of oviductal fluid extracellular vesicles from isthmus, to in vitro culture of bovine embryos in the absence of serum improves the development and quality of the embryos produced. 2023-02-20T07:14:51Z 2023-02-20T07:14:51Z 2017 artículo Reproduction 153: 461-470 (2017) 1470-1626 http://hdl.handle.net/20.500.12792/2418 http://hdl.handle.net/10261/291139 10.1530/REP-16-0384 1741-7899 en none BioScientifica |
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The aim of this study was to evaluate the effect of extracellular vesicles (EV) from oviductal fluid (OF), either from the ampulla or isthmus, on the development and quality of in vitro-cultured bovine embryos. Zygotes were cultured in synthetic oviduct fluid (SOF+3mg/mL BSA) without calf serum (C− group), in the presence of 3×105 EV/mL from ampullary or isthmic OF at either 1×104g (10K) or 1×105 g (100K), and compared with SOF+5% FCS (C+ group). OF-EV size and concentration were assessed by electron microscopy and nanotracking analysis system. Embryo development was recorded on Days 7–9, and blastocyst quality was assessed through cryotolerance and gene expression analysis. Lower blastocyst yield was observed on Day 7 in the C− and OF-EV groups (12.0–14.3%) compared with C+ (20.6%); however, these differences were compensated at Days 8 and 9 (Day 9: 28.5–30.8%). Importantly, the survival rate of blastocysts produced with isthmic 100K OF-EV was higher than that of C+ and C− group at 72h after vitrification and warming (80.1 vs 34.5 and 50.5% respectively, P < 0.05). In terms of gene expression, blastocysts produced in the presence of 100 K isthmic OF-EV upregulated the water channel AQP3 and DNMT3A and SNRPN transcripts compared with the C+, with the expression in C- being intermediate. The lipid receptor LDLR was downregulated in C+ compared with all other groups. In conclusion, the addition of oviductal fluid extracellular vesicles from isthmus, to in vitro culture of bovine embryos in the absence of serum improves the development and quality of the embryos produced. |
format |
artículo |
author |
Lopera-Vasquez, R. Hamdi, M. Maillo, V. Gutiérrez Adán, Alfonso Bermejo Álvarez, Pablo Angel Ramírez, M. Yáñez-Mó, M. Rizos Dimitrios, Dimitrios |
spellingShingle |
Lopera-Vasquez, R. Hamdi, M. Maillo, V. Gutiérrez Adán, Alfonso Bermejo Álvarez, Pablo Angel Ramírez, M. Yáñez-Mó, M. Rizos Dimitrios, Dimitrios Effect of bovine oviductal extracellular vesicles on embryo development and quality in vitro |
author_facet |
Lopera-Vasquez, R. Hamdi, M. Maillo, V. Gutiérrez Adán, Alfonso Bermejo Álvarez, Pablo Angel Ramírez, M. Yáñez-Mó, M. Rizos Dimitrios, Dimitrios |
author_sort |
Lopera-Vasquez, R. |
title |
Effect of bovine oviductal extracellular vesicles on embryo development and quality in vitro |
title_short |
Effect of bovine oviductal extracellular vesicles on embryo development and quality in vitro |
title_full |
Effect of bovine oviductal extracellular vesicles on embryo development and quality in vitro |
title_fullStr |
Effect of bovine oviductal extracellular vesicles on embryo development and quality in vitro |
title_full_unstemmed |
Effect of bovine oviductal extracellular vesicles on embryo development and quality in vitro |
title_sort |
effect of bovine oviductal extracellular vesicles on embryo development and quality in vitro |
publisher |
BioScientifica |
publishDate |
2017 |
url |
http://hdl.handle.net/20.500.12792/2418 http://hdl.handle.net/10261/291139 |
work_keys_str_mv |
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