Different influence of ovine estrus synchronization treatments on caruncular early angiogenesis
Summary. The present study compares two protocols for ovine estrus synchronization by assessing the caruncular angiogenic response to the establishment of pregnancy. The analysis consisted of the immunohistochemical evaluation of Vascular Endothelial Growth Factor (VEGF), Platelet Endothelial Cell Adhesion Molecule-1 (PECAM-1, CD31) and Von Willebrand Factor (vWF) in ovine caruncular stroma. A flock of thirty-eight adult ewes was divided in two groups and synchronized with either progestagens (Group P) or prostaglandin analogues (Group PG). Immunohistochemistry was performed in uterine samples obtained from pregnant ewes (P, n=15; PG, n=13) on days 15 post coitus (pc), 17pc and 21pc (day 0 =day of estrus). Each factor was assessed by total vascular density (TVD, total positive blood vessels/mm2), capillary vascular density (CVD, positive blood capillaries/mm2) and arteriolar vascular density (AVD, positive arterioles/mm2). Group P demonstrated higher VEGF-CVD (P=0.045) when compared to prostaglandin treated animals. Vascular CD31-expression decreased on days 15pc and 21pc (TVD, P=0.007 and CVD, P=0.014) in both groups. vWF analysis did not show significant differences between groups or days of study. These results demonstrate a different influence of progestagen-based and prostaglandin analogues-based synchronization treatments over VEGF vascular expression during caruncular development taking place in response to pregnancy establishment. In addition, observations pointed out in this study support the involvement of CD31 in the angiogenic stimulus that occurs during early maternal placentation in the ewe.
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Universidad de Murcia
2013
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Subjects: | Ewe, Angiogenesis, Early placentation, Estrus synchronization, |
Online Access: | http://hdl.handle.net/20.500.12792/3936 http://hdl.handle.net/10261/290958 |
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dig-inia-es-10261-2909582023-02-17T12:31:22Z Different influence of ovine estrus synchronization treatments on caruncular early angiogenesis Ruiz-González, I. Sánchez, M. A. García-Fernández, R. A. García-Palencia, P. Sánchez, B. González De Bulnes, Antonio Flores, J. M. Ewe Angiogenesis Early placentation Estrus synchronization Summary. The present study compares two protocols for ovine estrus synchronization by assessing the caruncular angiogenic response to the establishment of pregnancy. The analysis consisted of the immunohistochemical evaluation of Vascular Endothelial Growth Factor (VEGF), Platelet Endothelial Cell Adhesion Molecule-1 (PECAM-1, CD31) and Von Willebrand Factor (vWF) in ovine caruncular stroma. A flock of thirty-eight adult ewes was divided in two groups and synchronized with either progestagens (Group P) or prostaglandin analogues (Group PG). Immunohistochemistry was performed in uterine samples obtained from pregnant ewes (P, n=15; PG, n=13) on days 15 post coitus (pc), 17pc and 21pc (day 0 =day of estrus). Each factor was assessed by total vascular density (TVD, total positive blood vessels/mm2), capillary vascular density (CVD, positive blood capillaries/mm2) and arteriolar vascular density (AVD, positive arterioles/mm2). Group P demonstrated higher VEGF-CVD (P=0.045) when compared to prostaglandin treated animals. Vascular CD31-expression decreased on days 15pc and 21pc (TVD, P=0.007 and CVD, P=0.014) in both groups. vWF analysis did not show significant differences between groups or days of study. These results demonstrate a different influence of progestagen-based and prostaglandin analogues-based synchronization treatments over VEGF vascular expression during caruncular development taking place in response to pregnancy establishment. In addition, observations pointed out in this study support the involvement of CD31 in the angiogenic stimulus that occurs during early maternal placentation in the ewe. 2023-02-17T12:31:22Z 2023-02-17T12:31:22Z 2013 artículo Histology and Histopathology 28(3): 373-383 (2013) 0213-3911 http://hdl.handle.net/20.500.12792/3936 http://hdl.handle.net/10261/290958 10.14670/HH-28.373 1699-584 en open Universidad de Murcia |
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Ewe Angiogenesis Early placentation Estrus synchronization Ewe Angiogenesis Early placentation Estrus synchronization |
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Ewe Angiogenesis Early placentation Estrus synchronization Ewe Angiogenesis Early placentation Estrus synchronization Ruiz-González, I. Sánchez, M. A. García-Fernández, R. A. García-Palencia, P. Sánchez, B. González De Bulnes, Antonio Flores, J. M. Different influence of ovine estrus synchronization treatments on caruncular early angiogenesis |
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Summary. The present study compares two protocols for ovine estrus synchronization by assessing the caruncular angiogenic response to the establishment of pregnancy. The analysis consisted of the immunohistochemical evaluation of Vascular Endothelial Growth Factor (VEGF), Platelet Endothelial Cell Adhesion Molecule-1 (PECAM-1, CD31) and Von Willebrand Factor (vWF) in ovine caruncular stroma. A flock of thirty-eight adult ewes was divided in two groups and synchronized with either progestagens (Group P) or prostaglandin analogues (Group PG). Immunohistochemistry was performed in uterine samples obtained from pregnant ewes (P, n=15; PG, n=13) on days 15 post coitus (pc), 17pc and 21pc (day 0 =day of estrus). Each factor was assessed by total vascular density (TVD, total positive blood vessels/mm2), capillary vascular density (CVD, positive blood capillaries/mm2) and arteriolar vascular density (AVD, positive arterioles/mm2). Group P demonstrated higher VEGF-CVD (P=0.045) when compared to prostaglandin treated animals. Vascular CD31-expression decreased on days 15pc and 21pc (TVD, P=0.007 and CVD, P=0.014) in both groups. vWF analysis did not show significant differences between groups or days of study. These results demonstrate a different influence of progestagen-based and prostaglandin analogues-based synchronization treatments over VEGF vascular expression during caruncular development taking place in response to pregnancy establishment. In addition, observations pointed out in this study support the involvement of CD31 in the angiogenic stimulus that occurs during early maternal placentation in the ewe. |
format |
artículo |
topic_facet |
Ewe Angiogenesis Early placentation Estrus synchronization |
author |
Ruiz-González, I. Sánchez, M. A. García-Fernández, R. A. García-Palencia, P. Sánchez, B. González De Bulnes, Antonio Flores, J. M. |
author_facet |
Ruiz-González, I. Sánchez, M. A. García-Fernández, R. A. García-Palencia, P. Sánchez, B. González De Bulnes, Antonio Flores, J. M. |
author_sort |
Ruiz-González, I. |
title |
Different influence of ovine estrus synchronization treatments on caruncular early angiogenesis |
title_short |
Different influence of ovine estrus synchronization treatments on caruncular early angiogenesis |
title_full |
Different influence of ovine estrus synchronization treatments on caruncular early angiogenesis |
title_fullStr |
Different influence of ovine estrus synchronization treatments on caruncular early angiogenesis |
title_full_unstemmed |
Different influence of ovine estrus synchronization treatments on caruncular early angiogenesis |
title_sort |
different influence of ovine estrus synchronization treatments on caruncular early angiogenesis |
publisher |
Universidad de Murcia |
publishDate |
2013 |
url |
http://hdl.handle.net/20.500.12792/3936 http://hdl.handle.net/10261/290958 |
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