D-Root A system for cultivating plants with the roots in darkness or under different light conditions
In nature roots grow in the dark and away from light (negative phototropism). However, most current research in root biology has been carried out with the root system grown in the presence of light. Here, we have engineered a device, called Dark-Root (D-Root), to grow plants in vitro with the aerial part exposed to the normal light/dark photoperiod while the roots are in the dark or exposed to specific wavelengths or light intensities. D-Root provides an efficient system for cultivating a large number of seedlings and easily characterizing root architecture in the dark. At the morphological level, root illumination shortens root length and promotes early emergence of lateral roots, therefore inducing expansion of the root system. Surprisingly, root illumination also affects shoot development, including flowering time. Our analyses also show that root illumination alters the proper response to hormones or abiotic stress (e.g. salt or osmotic stress) and nutrient starvation, enhancing inhibition of root growth. In conclusion, D-Root provides a growing system closer to the natural one for assaying Arabidopsis plants, and therefore its use will contribute to a better understanding of the mechanisms involved in root development, hormonal signaling and stress responses. © 2015 The Authors The Plant Journal © 2015 John Wiley & Sons Ltd.
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Format: | artículo biblioteca |
Language: | English |
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Wiley
2015
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Subjects: | Root development, Light, Hormone response, Abiotic stress, Reactive oxygen species, Technical Advance, |
Online Access: | http://hdl.handle.net/20.500.12792/4324 http://hdl.handle.net/10261/290896 |
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dig-inia-es-10261-2908962023-02-17T12:30:42Z D-Root A system for cultivating plants with the roots in darkness or under different light conditions Silva-Navas, J. Moreno-Risueno, M. A. Manzano, C. Pallero-Baena, M. Navarro Neila, Sara Téllez-Robledo, B. Garcia-Mina, J. M. Baigorri, R. Gallego, F. J. del Pozo, J. C. Root development Light Hormone response Abiotic stress Reactive oxygen species Technical Advance In nature roots grow in the dark and away from light (negative phototropism). However, most current research in root biology has been carried out with the root system grown in the presence of light. Here, we have engineered a device, called Dark-Root (D-Root), to grow plants in vitro with the aerial part exposed to the normal light/dark photoperiod while the roots are in the dark or exposed to specific wavelengths or light intensities. D-Root provides an efficient system for cultivating a large number of seedlings and easily characterizing root architecture in the dark. At the morphological level, root illumination shortens root length and promotes early emergence of lateral roots, therefore inducing expansion of the root system. Surprisingly, root illumination also affects shoot development, including flowering time. Our analyses also show that root illumination alters the proper response to hormones or abiotic stress (e.g. salt or osmotic stress) and nutrient starvation, enhancing inhibition of root growth. In conclusion, D-Root provides a growing system closer to the natural one for assaying Arabidopsis plants, and therefore its use will contribute to a better understanding of the mechanisms involved in root development, hormonal signaling and stress responses. © 2015 The Authors The Plant Journal © 2015 John Wiley & Sons Ltd. 2023-02-17T12:30:42Z 2023-02-17T12:30:42Z 2015 artículo Plant Journal 84: 244-255 (2015) 0960-7412 http://hdl.handle.net/20.500.12792/4324 http://hdl.handle.net/10261/290896 10.1111/tpj.12998 1365-313X en none Wiley |
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Root development Light Hormone response Abiotic stress Reactive oxygen species Technical Advance Root development Light Hormone response Abiotic stress Reactive oxygen species Technical Advance |
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Root development Light Hormone response Abiotic stress Reactive oxygen species Technical Advance Root development Light Hormone response Abiotic stress Reactive oxygen species Technical Advance Silva-Navas, J. Moreno-Risueno, M. A. Manzano, C. Pallero-Baena, M. Navarro Neila, Sara Téllez-Robledo, B. Garcia-Mina, J. M. Baigorri, R. Gallego, F. J. del Pozo, J. C. D-Root A system for cultivating plants with the roots in darkness or under different light conditions |
description |
In nature roots grow in the dark and away from light (negative phototropism). However, most current research in root biology has been carried out with the root system grown in the presence of light. Here, we have engineered a device, called Dark-Root (D-Root), to grow plants in vitro with the aerial part exposed to the normal light/dark photoperiod while the roots are in the dark or exposed to specific wavelengths or light intensities. D-Root provides an efficient system for cultivating a large number of seedlings and easily characterizing root architecture in the dark. At the morphological level, root illumination shortens root length and promotes early emergence of lateral roots, therefore inducing expansion of the root system. Surprisingly, root illumination also affects shoot development, including flowering time. Our analyses also show that root illumination alters the proper response to hormones or abiotic stress (e.g. salt or osmotic stress) and nutrient starvation, enhancing inhibition of root growth. In conclusion, D-Root provides a growing system closer to the natural one for assaying Arabidopsis plants, and therefore its use will contribute to a better understanding of the mechanisms involved in root development, hormonal signaling and stress responses. © 2015 The Authors The Plant Journal © 2015 John Wiley & Sons Ltd. |
format |
artículo |
topic_facet |
Root development Light Hormone response Abiotic stress Reactive oxygen species Technical Advance |
author |
Silva-Navas, J. Moreno-Risueno, M. A. Manzano, C. Pallero-Baena, M. Navarro Neila, Sara Téllez-Robledo, B. Garcia-Mina, J. M. Baigorri, R. Gallego, F. J. del Pozo, J. C. |
author_facet |
Silva-Navas, J. Moreno-Risueno, M. A. Manzano, C. Pallero-Baena, M. Navarro Neila, Sara Téllez-Robledo, B. Garcia-Mina, J. M. Baigorri, R. Gallego, F. J. del Pozo, J. C. |
author_sort |
Silva-Navas, J. |
title |
D-Root A system for cultivating plants with the roots in darkness or under different light conditions |
title_short |
D-Root A system for cultivating plants with the roots in darkness or under different light conditions |
title_full |
D-Root A system for cultivating plants with the roots in darkness or under different light conditions |
title_fullStr |
D-Root A system for cultivating plants with the roots in darkness or under different light conditions |
title_full_unstemmed |
D-Root A system for cultivating plants with the roots in darkness or under different light conditions |
title_sort |
d-root a system for cultivating plants with the roots in darkness or under different light conditions |
publisher |
Wiley |
publishDate |
2015 |
url |
http://hdl.handle.net/20.500.12792/4324 http://hdl.handle.net/10261/290896 |
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