The microbiota of eight species of dehydrated edible seaweeds from North West Spain

The microbiota of eight species (Chondrus crispus, Himanthalia elongata, Laminaria ochroleuca, Palmaria palmata, Porphyra umbilicalis, Saccharina latissima, Ulva lactuca and Undaria pinnatifida) of edible seaweeds collected in North West Spain, marketed as dehydrated product, was quantitatively determined on nine solid media. Representative colonies were selected from solid culture media. The isolated microorganisms were identified by means of morphological characteristics, 16S rDNA sequencing and biochemical tests. U. pinnatifida was the seaweed species showing the most abundant microbial population, with counts on Marine agar up to 7.7 log cfu/g in individual samples and 5.0 log cfu/g as the mean value, and counts of coliforms up to 4.6 log cfu/g in individual samples and 2.4 log cfu/g as the mean value. The 225 identified bacterial isolates belonged to 11 families, 27 genera and 56 species. Bacillaceae was the family accounting for the highest number of isolates (111) followed by Enterobacteriaceae (60), Bacillales Family XII Incertae Sedis (20), Planococcaceae (11), Moraxellaceae (7), Paenibacillaceae (5) and Pseudomonadaceae (5). Bacterial species showing the highest occurrence in dehydrated seaweeds were Bacillus megaterium, B. licheniformis, Pantoea sp. and termoresistant Pantoea sp. Four of the Bacillus species isolated from dehydrated seaweeds (B. cereus, B. licheniformis, B. pumilus and B. subtilis) are among those containing strains considered to be foodborne pathogens and nine of the isolated non-Bacillales bacterial species have been reported to contain human opportunistic pathogenic strains.

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Main Authors: del Olmo, A., Picón Gálvez, Antonia María, Núñez Gutiérrez, Manuel
Format: artículo biblioteca
Language:English
Published: Elsevier 2018
Subjects:Microbiota, Seaweeds, 16S rDNA sequencing, Bacillaceae, Enterobacteriaceae,
Online Access:http://hdl.handle.net/20.500.12792/939
http://hdl.handle.net/10261/290862
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spelling dig-inia-es-10261-2908622023-02-17T12:30:14Z The microbiota of eight species of dehydrated edible seaweeds from North West Spain del Olmo, A. Picón Gálvez, Antonia María Núñez Gutiérrez, Manuel Microbiota Seaweeds 16S rDNA sequencing Bacillaceae Enterobacteriaceae The microbiota of eight species (Chondrus crispus, Himanthalia elongata, Laminaria ochroleuca, Palmaria palmata, Porphyra umbilicalis, Saccharina latissima, Ulva lactuca and Undaria pinnatifida) of edible seaweeds collected in North West Spain, marketed as dehydrated product, was quantitatively determined on nine solid media. Representative colonies were selected from solid culture media. The isolated microorganisms were identified by means of morphological characteristics, 16S rDNA sequencing and biochemical tests. U. pinnatifida was the seaweed species showing the most abundant microbial population, with counts on Marine agar up to 7.7 log cfu/g in individual samples and 5.0 log cfu/g as the mean value, and counts of coliforms up to 4.6 log cfu/g in individual samples and 2.4 log cfu/g as the mean value. The 225 identified bacterial isolates belonged to 11 families, 27 genera and 56 species. Bacillaceae was the family accounting for the highest number of isolates (111) followed by Enterobacteriaceae (60), Bacillales Family XII Incertae Sedis (20), Planococcaceae (11), Moraxellaceae (7), Paenibacillaceae (5) and Pseudomonadaceae (5). Bacterial species showing the highest occurrence in dehydrated seaweeds were Bacillus megaterium, B. licheniformis, Pantoea sp. and termoresistant Pantoea sp. Four of the Bacillus species isolated from dehydrated seaweeds (B. cereus, B. licheniformis, B. pumilus and B. subtilis) are among those containing strains considered to be foodborne pathogens and nine of the isolated non-Bacillales bacterial species have been reported to contain human opportunistic pathogenic strains. 2023-02-17T12:30:14Z 2023-02-17T12:30:14Z 2018 artículo Food Microbiology 70: e224e231 (2018) 0740-0020 http://hdl.handle.net/20.500.12792/939 http://hdl.handle.net/10261/290862 10.1016/j.fm.2017.10.009 1095-9998 en none Elsevier
institution INIA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-inia-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del INIA España
language English
topic Microbiota
Seaweeds
16S rDNA sequencing
Bacillaceae
Enterobacteriaceae
Microbiota
Seaweeds
16S rDNA sequencing
Bacillaceae
Enterobacteriaceae
spellingShingle Microbiota
Seaweeds
16S rDNA sequencing
Bacillaceae
Enterobacteriaceae
Microbiota
Seaweeds
16S rDNA sequencing
Bacillaceae
Enterobacteriaceae
del Olmo, A.
Picón Gálvez, Antonia María
Núñez Gutiérrez, Manuel
The microbiota of eight species of dehydrated edible seaweeds from North West Spain
description The microbiota of eight species (Chondrus crispus, Himanthalia elongata, Laminaria ochroleuca, Palmaria palmata, Porphyra umbilicalis, Saccharina latissima, Ulva lactuca and Undaria pinnatifida) of edible seaweeds collected in North West Spain, marketed as dehydrated product, was quantitatively determined on nine solid media. Representative colonies were selected from solid culture media. The isolated microorganisms were identified by means of morphological characteristics, 16S rDNA sequencing and biochemical tests. U. pinnatifida was the seaweed species showing the most abundant microbial population, with counts on Marine agar up to 7.7 log cfu/g in individual samples and 5.0 log cfu/g as the mean value, and counts of coliforms up to 4.6 log cfu/g in individual samples and 2.4 log cfu/g as the mean value. The 225 identified bacterial isolates belonged to 11 families, 27 genera and 56 species. Bacillaceae was the family accounting for the highest number of isolates (111) followed by Enterobacteriaceae (60), Bacillales Family XII Incertae Sedis (20), Planococcaceae (11), Moraxellaceae (7), Paenibacillaceae (5) and Pseudomonadaceae (5). Bacterial species showing the highest occurrence in dehydrated seaweeds were Bacillus megaterium, B. licheniformis, Pantoea sp. and termoresistant Pantoea sp. Four of the Bacillus species isolated from dehydrated seaweeds (B. cereus, B. licheniformis, B. pumilus and B. subtilis) are among those containing strains considered to be foodborne pathogens and nine of the isolated non-Bacillales bacterial species have been reported to contain human opportunistic pathogenic strains.
format artículo
topic_facet Microbiota
Seaweeds
16S rDNA sequencing
Bacillaceae
Enterobacteriaceae
author del Olmo, A.
Picón Gálvez, Antonia María
Núñez Gutiérrez, Manuel
author_facet del Olmo, A.
Picón Gálvez, Antonia María
Núñez Gutiérrez, Manuel
author_sort del Olmo, A.
title The microbiota of eight species of dehydrated edible seaweeds from North West Spain
title_short The microbiota of eight species of dehydrated edible seaweeds from North West Spain
title_full The microbiota of eight species of dehydrated edible seaweeds from North West Spain
title_fullStr The microbiota of eight species of dehydrated edible seaweeds from North West Spain
title_full_unstemmed The microbiota of eight species of dehydrated edible seaweeds from North West Spain
title_sort microbiota of eight species of dehydrated edible seaweeds from north west spain
publisher Elsevier
publishDate 2018
url http://hdl.handle.net/20.500.12792/939
http://hdl.handle.net/10261/290862
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