Directions and applications of CRISPR technology in livestock research
The ablation (KO) or targeted insertion (KI) of specific genes or sequences has been essential to test their roles on a particular biological process. Unfortunately, such genome modifications have been largely limited to the mouse model, as the only way to achieve targeted mutagenesis in other mammals required from somatic cell nuclear transfer, a time- and resource-consuming technique. This difficulty has left research in livestock species largely devoided of KO and targeted KI models, crucial tools to uncover the molecular roots of any physiological or pathological process. Luckily, the eruption of site-specific endonucleases, and particularly CRISPR technology, has empowered farm animal scientists to consider projects that could not develop before. In this sense, the availability of genome modification in livestock species is meant to change the way research is performed on many fields, switching from descriptive and correlational approaches to experimental research. In this review we will provide some guidance about how the genome can be edited by CRISPR and the possible strategies to achieve KO or KI, paying special attention to an initially overlooked phenomenon: mosaicism. Mosaicism is produced when the zygote´s genome edition occurs after its DNA has replicated, and is characterized by the presence of more than two alleles in the same individual, an undesirable outcome when attempting direct KO generation. Finally, the possible applications on different fields of livestock research, such as reproduction or infectious diseases are discussed.
Main Authors: | , , , |
---|---|
Format: | artículo biblioteca |
Language: | English |
Published: |
Brazilian College of Animal Reproduction
2018
|
Online Access: | http://hdl.handle.net/20.500.12792/597 http://hdl.handle.net/10261/290811 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-inia-es-10261-290811 |
---|---|
record_format |
koha |
spelling |
dig-inia-es-10261-2908112023-02-17T12:29:40Z Directions and applications of CRISPR technology in livestock research Lamas Toranzo, Ismael Ramos Ibeas, Priscila Pericuesta Camacho, Eva Bermejo Álvarez, Pablo The ablation (KO) or targeted insertion (KI) of specific genes or sequences has been essential to test their roles on a particular biological process. Unfortunately, such genome modifications have been largely limited to the mouse model, as the only way to achieve targeted mutagenesis in other mammals required from somatic cell nuclear transfer, a time- and resource-consuming technique. This difficulty has left research in livestock species largely devoided of KO and targeted KI models, crucial tools to uncover the molecular roots of any physiological or pathological process. Luckily, the eruption of site-specific endonucleases, and particularly CRISPR technology, has empowered farm animal scientists to consider projects that could not develop before. In this sense, the availability of genome modification in livestock species is meant to change the way research is performed on many fields, switching from descriptive and correlational approaches to experimental research. In this review we will provide some guidance about how the genome can be edited by CRISPR and the possible strategies to achieve KO or KI, paying special attention to an initially overlooked phenomenon: mosaicism. Mosaicism is produced when the zygote´s genome edition occurs after its DNA has replicated, and is characterized by the presence of more than two alleles in the same individual, an undesirable outcome when attempting direct KO generation. Finally, the possible applications on different fields of livestock research, such as reproduction or infectious diseases are discussed. 2023-02-17T12:29:40Z 2023-02-17T12:29:40Z 2018 artículo Animal Reproduction 15 (3) :292-300 (2018) 1806-9614 http://hdl.handle.net/20.500.12792/597 http://hdl.handle.net/10261/290811 10.21451/1984-3143-AR2018-0075 1984-3143 en none Brazilian College of Animal Reproduction |
institution |
INIA ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-inia-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del INIA España |
language |
English |
description |
The ablation (KO) or targeted insertion (KI) of specific genes or sequences has been essential to test their roles on a particular biological process. Unfortunately, such genome modifications have been largely limited to the mouse model, as the only way to achieve targeted mutagenesis in other mammals required from somatic cell nuclear transfer, a time- and resource-consuming technique. This difficulty has left research in livestock species largely devoided of KO and targeted KI models, crucial tools to uncover the molecular roots of any physiological or pathological process. Luckily, the eruption of site-specific endonucleases, and particularly CRISPR technology, has empowered farm animal scientists to consider projects that could not develop before. In this sense, the availability of genome modification in livestock species is meant to change the way research is performed on many fields, switching from descriptive and correlational approaches to experimental research. In this review we will provide some guidance about how the genome can be edited by CRISPR and the possible strategies to achieve KO or KI, paying special attention to an initially overlooked phenomenon: mosaicism. Mosaicism is produced when the zygote´s genome edition occurs after its DNA has replicated, and is characterized by the presence of more than two alleles in the same individual, an undesirable outcome when attempting direct KO generation. Finally, the possible applications on different fields of livestock research, such as reproduction or infectious diseases are discussed. |
format |
artículo |
author |
Lamas Toranzo, Ismael Ramos Ibeas, Priscila Pericuesta Camacho, Eva Bermejo Álvarez, Pablo |
spellingShingle |
Lamas Toranzo, Ismael Ramos Ibeas, Priscila Pericuesta Camacho, Eva Bermejo Álvarez, Pablo Directions and applications of CRISPR technology in livestock research |
author_facet |
Lamas Toranzo, Ismael Ramos Ibeas, Priscila Pericuesta Camacho, Eva Bermejo Álvarez, Pablo |
author_sort |
Lamas Toranzo, Ismael |
title |
Directions and applications of CRISPR technology in livestock research |
title_short |
Directions and applications of CRISPR technology in livestock research |
title_full |
Directions and applications of CRISPR technology in livestock research |
title_fullStr |
Directions and applications of CRISPR technology in livestock research |
title_full_unstemmed |
Directions and applications of CRISPR technology in livestock research |
title_sort |
directions and applications of crispr technology in livestock research |
publisher |
Brazilian College of Animal Reproduction |
publishDate |
2018 |
url |
http://hdl.handle.net/20.500.12792/597 http://hdl.handle.net/10261/290811 |
work_keys_str_mv |
AT lamastoranzoismael directionsandapplicationsofcrisprtechnologyinlivestockresearch AT ramosibeaspriscila directionsandapplicationsofcrisprtechnologyinlivestockresearch AT pericuestacamachoeva directionsandapplicationsofcrisprtechnologyinlivestockresearch AT bermejoalvarezpablo directionsandapplicationsofcrisprtechnologyinlivestockresearch |
_version_ |
1767603138006614016 |