Characterization of five monoclonal antibodies specific for swine class II major histocompatibility antigens and crossreactivity studies with leukocytes of domestic animals

A set of five monoclonal antibodies (mAb) against porcine major histocompatibility complex (MHC), or swine leukocyte antigens (SLA), class II molecules has been characterized. These mAbs appear to recognize monomorphic determinants on SLA-DR (2F4, 1F12 and 2E9/13) and SLADQ (BL2H5 and BL4H2) molecules, as assessed by flow cytometry and immunoprecipitation. By Western blot, the 2F4,1F12, BL2H5 and BL4H2 epitopes were located on the βchains of these molecules. mAbs 2F4 and 1F12 crossreact with leucocytes of dog, cattle, horse and human; mAbs 2E9/13, BL2H5 and BL4H2 bind leucocytes of cattle but not those of human, dog and horse. These mAbs effectively blocked the mixed lymphocyte reaction and the proliferative response to viral antigens (African swine fever virus) and to staphylococcal enterotoxin B. Therefore, these mAbs can be useful reagents for studying MHC class II molecules of pig and crossreactive species, and the immunological processes where they are involved.

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Main Authors: Bullido, R., Doménech, N., Alvarez, B., Alonso, F., Babín, M., Ezquerra Martínez, Ángel, Ortuño, E., Domínguez, J.
Format: artículo biblioteca
Language:English
Published: Elsevier 1997
Subjects:Swine, Monoclonal antibodies, Swine leukocyte antigen class II molecules, Crossreactivity, Domestic animals, Leukocytes, Swine leukocyte antigen class II transfectants,
Online Access:http://hdl.handle.net/20.500.12792/4885
http://hdl.handle.net/10261/290764
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spelling dig-inia-es-10261-2907642023-02-17T12:29:08Z Characterization of five monoclonal antibodies specific for swine class II major histocompatibility antigens and crossreactivity studies with leukocytes of domestic animals Bullido, R. Doménech, N. Alvarez, B. Alonso, F. Babín, M. Ezquerra Martínez, Ángel Ortuño, E. Domínguez, J. Swine Monoclonal antibodies Swine leukocyte antigen class II molecules Crossreactivity Domestic animals Leukocytes Swine leukocyte antigen class II transfectants A set of five monoclonal antibodies (mAb) against porcine major histocompatibility complex (MHC), or swine leukocyte antigens (SLA), class II molecules has been characterized. These mAbs appear to recognize monomorphic determinants on SLA-DR (2F4, 1F12 and 2E9/13) and SLADQ (BL2H5 and BL4H2) molecules, as assessed by flow cytometry and immunoprecipitation. By Western blot, the 2F4,1F12, BL2H5 and BL4H2 epitopes were located on the βchains of these molecules. mAbs 2F4 and 1F12 crossreact with leucocytes of dog, cattle, horse and human; mAbs 2E9/13, BL2H5 and BL4H2 bind leucocytes of cattle but not those of human, dog and horse. These mAbs effectively blocked the mixed lymphocyte reaction and the proliferative response to viral antigens (African swine fever virus) and to staphylococcal enterotoxin B. Therefore, these mAbs can be useful reagents for studying MHC class II molecules of pig and crossreactive species, and the immunological processes where they are involved. 2023-02-17T12:29:08Z 2023-02-17T12:29:08Z 1997 artículo Developmental and Comparative Immunology 21(3): 311-322 (1997) 0145-305X http://hdl.handle.net/20.500.12792/4885 http://hdl.handle.net/10261/290764 10.1016/S0145-305X(97)00008-6 en none Elsevier
institution INIA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-inia-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del INIA España
language English
topic Swine
Monoclonal antibodies
Swine leukocyte antigen class II molecules
Crossreactivity
Domestic animals
Leukocytes
Swine leukocyte antigen class II transfectants
Swine
Monoclonal antibodies
Swine leukocyte antigen class II molecules
Crossreactivity
Domestic animals
Leukocytes
Swine leukocyte antigen class II transfectants
spellingShingle Swine
Monoclonal antibodies
Swine leukocyte antigen class II molecules
Crossreactivity
Domestic animals
Leukocytes
Swine leukocyte antigen class II transfectants
Swine
Monoclonal antibodies
Swine leukocyte antigen class II molecules
Crossreactivity
Domestic animals
Leukocytes
Swine leukocyte antigen class II transfectants
Bullido, R.
Doménech, N.
Alvarez, B.
Alonso, F.
Babín, M.
Ezquerra Martínez, Ángel
Ortuño, E.
Domínguez, J.
Characterization of five monoclonal antibodies specific for swine class II major histocompatibility antigens and crossreactivity studies with leukocytes of domestic animals
description A set of five monoclonal antibodies (mAb) against porcine major histocompatibility complex (MHC), or swine leukocyte antigens (SLA), class II molecules has been characterized. These mAbs appear to recognize monomorphic determinants on SLA-DR (2F4, 1F12 and 2E9/13) and SLADQ (BL2H5 and BL4H2) molecules, as assessed by flow cytometry and immunoprecipitation. By Western blot, the 2F4,1F12, BL2H5 and BL4H2 epitopes were located on the βchains of these molecules. mAbs 2F4 and 1F12 crossreact with leucocytes of dog, cattle, horse and human; mAbs 2E9/13, BL2H5 and BL4H2 bind leucocytes of cattle but not those of human, dog and horse. These mAbs effectively blocked the mixed lymphocyte reaction and the proliferative response to viral antigens (African swine fever virus) and to staphylococcal enterotoxin B. Therefore, these mAbs can be useful reagents for studying MHC class II molecules of pig and crossreactive species, and the immunological processes where they are involved.
format artículo
topic_facet Swine
Monoclonal antibodies
Swine leukocyte antigen class II molecules
Crossreactivity
Domestic animals
Leukocytes
Swine leukocyte antigen class II transfectants
author Bullido, R.
Doménech, N.
Alvarez, B.
Alonso, F.
Babín, M.
Ezquerra Martínez, Ángel
Ortuño, E.
Domínguez, J.
author_facet Bullido, R.
Doménech, N.
Alvarez, B.
Alonso, F.
Babín, M.
Ezquerra Martínez, Ángel
Ortuño, E.
Domínguez, J.
author_sort Bullido, R.
title Characterization of five monoclonal antibodies specific for swine class II major histocompatibility antigens and crossreactivity studies with leukocytes of domestic animals
title_short Characterization of five monoclonal antibodies specific for swine class II major histocompatibility antigens and crossreactivity studies with leukocytes of domestic animals
title_full Characterization of five monoclonal antibodies specific for swine class II major histocompatibility antigens and crossreactivity studies with leukocytes of domestic animals
title_fullStr Characterization of five monoclonal antibodies specific for swine class II major histocompatibility antigens and crossreactivity studies with leukocytes of domestic animals
title_full_unstemmed Characterization of five monoclonal antibodies specific for swine class II major histocompatibility antigens and crossreactivity studies with leukocytes of domestic animals
title_sort characterization of five monoclonal antibodies specific for swine class ii major histocompatibility antigens and crossreactivity studies with leukocytes of domestic animals
publisher Elsevier
publishDate 1997
url http://hdl.handle.net/20.500.12792/4885
http://hdl.handle.net/10261/290764
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