The effect of bovine embryo culture without proteins supplements until day 4 on transcription level of hyaluronan synthases, receptors and mtDNA content
The effect of bovine embryo culture on a flat surface, (without a surface-active compound) on the level of mRNA expression of hyaluronan (HA) synthases (Has1, Has2 and Has3), Ha receptors RHAMM and C44 receptors was evaluated by mitochondrial DNA concentration and in vitro development. Cultures were evaluated up to 96 h post-insemination (hpi) using SOFaa medium. Of the three Has isoforms, Has2 expression only increased in the bovine serum albumin (BSA)-only supplemented groups regardless of time of BSA addition. Expression of RHAMM receptors was highly dependent on the addition of HA, irrespective of the presence of BSA in the medium. In contrast, expression of the CD44 receptor gene was not affected by any treatment. The cleavage rates and number of embryos that developed to 8-cell stage by day 4 were not affected by lack of BSA in the medium, but increased numbers of blastocysts developed in medium supplemented with BSA from days 1 or 4 with or without HA than in medium that had HA only. Addition of both HA and BSA at day 4 increased mtDNA copy numbers at the blastocyst stage. Data suggest that the addition of BSA and/or HA at 96 hpi increased expression of RHAMM and Has2 genes, but not CD44, Has1 or Has3 genes. Higher expression levels of Has2 than Has1 and the three isoforms indicate that high- rather than low-molecular-weight HA should be used for preimplantation bovine embryo culture. Copyright © 2009 Cambridge University Press.
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Cambridge University Press
2010
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Subjects: | Culture, Embryo, Gene expression, Hyaluronan, Mitochondria, Synthases, |
Online Access: | http://hdl.handle.net/20.500.12792/3290 http://hdl.handle.net/10261/290633 |
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dig-inia-es-10261-2906332023-02-17T12:27:48Z The effect of bovine embryo culture without proteins supplements until day 4 on transcription level of hyaluronan synthases, receptors and mtDNA content Palasz, A. T. Beltrán Breña, Paula De la Fuente, J. Gutiérrez Adán, Alfonso Culture Embryo Gene expression Hyaluronan Mitochondria Synthases The effect of bovine embryo culture on a flat surface, (without a surface-active compound) on the level of mRNA expression of hyaluronan (HA) synthases (Has1, Has2 and Has3), Ha receptors RHAMM and C44 receptors was evaluated by mitochondrial DNA concentration and in vitro development. Cultures were evaluated up to 96 h post-insemination (hpi) using SOFaa medium. Of the three Has isoforms, Has2 expression only increased in the bovine serum albumin (BSA)-only supplemented groups regardless of time of BSA addition. Expression of RHAMM receptors was highly dependent on the addition of HA, irrespective of the presence of BSA in the medium. In contrast, expression of the CD44 receptor gene was not affected by any treatment. The cleavage rates and number of embryos that developed to 8-cell stage by day 4 were not affected by lack of BSA in the medium, but increased numbers of blastocysts developed in medium supplemented with BSA from days 1 or 4 with or without HA than in medium that had HA only. Addition of both HA and BSA at day 4 increased mtDNA copy numbers at the blastocyst stage. Data suggest that the addition of BSA and/or HA at 96 hpi increased expression of RHAMM and Has2 genes, but not CD44, Has1 or Has3 genes. Higher expression levels of Has2 than Has1 and the three isoforms indicate that high- rather than low-molecular-weight HA should be used for preimplantation bovine embryo culture. Copyright © 2009 Cambridge University Press. 2023-02-17T12:27:48Z 2023-02-17T12:27:48Z 2010 artículo Zygote 18: 121-129 (2010) 0967-1994 http://hdl.handle.net/20.500.12792/3290 http://hdl.handle.net/10261/290633 10.1017/S0967199409990128 1469-8730 en none Cambridge University Press |
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Culture Embryo Gene expression Hyaluronan Mitochondria Synthases Culture Embryo Gene expression Hyaluronan Mitochondria Synthases |
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Culture Embryo Gene expression Hyaluronan Mitochondria Synthases Culture Embryo Gene expression Hyaluronan Mitochondria Synthases Palasz, A. T. Beltrán Breña, Paula De la Fuente, J. Gutiérrez Adán, Alfonso The effect of bovine embryo culture without proteins supplements until day 4 on transcription level of hyaluronan synthases, receptors and mtDNA content |
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The effect of bovine embryo culture on a flat surface, (without a surface-active compound) on the level of mRNA expression of hyaluronan (HA) synthases (Has1, Has2 and Has3), Ha receptors RHAMM and C44 receptors was evaluated by mitochondrial DNA concentration and in vitro development. Cultures were evaluated up to 96 h post-insemination (hpi) using SOFaa medium. Of the three Has isoforms, Has2 expression only increased in the bovine serum albumin (BSA)-only supplemented groups regardless of time of BSA addition. Expression of RHAMM receptors was highly dependent on the addition of HA, irrespective of the presence of BSA in the medium. In contrast, expression of the CD44 receptor gene was not affected by any treatment. The cleavage rates and number of embryos that developed to 8-cell stage by day 4 were not affected by lack of BSA in the medium, but increased numbers of blastocysts developed in medium supplemented with BSA from days 1 or 4 with or without HA than in medium that had HA only. Addition of both HA and BSA at day 4 increased mtDNA copy numbers at the blastocyst stage. Data suggest that the addition of BSA and/or HA at 96 hpi increased expression of RHAMM and Has2 genes, but not CD44, Has1 or Has3 genes. Higher expression levels of Has2 than Has1 and the three isoforms indicate that high- rather than low-molecular-weight HA should be used for preimplantation bovine embryo culture. Copyright © 2009 Cambridge University Press. |
format |
artículo |
topic_facet |
Culture Embryo Gene expression Hyaluronan Mitochondria Synthases |
author |
Palasz, A. T. Beltrán Breña, Paula De la Fuente, J. Gutiérrez Adán, Alfonso |
author_facet |
Palasz, A. T. Beltrán Breña, Paula De la Fuente, J. Gutiérrez Adán, Alfonso |
author_sort |
Palasz, A. T. |
title |
The effect of bovine embryo culture without proteins supplements until day 4 on transcription level of hyaluronan synthases, receptors and mtDNA content |
title_short |
The effect of bovine embryo culture without proteins supplements until day 4 on transcription level of hyaluronan synthases, receptors and mtDNA content |
title_full |
The effect of bovine embryo culture without proteins supplements until day 4 on transcription level of hyaluronan synthases, receptors and mtDNA content |
title_fullStr |
The effect of bovine embryo culture without proteins supplements until day 4 on transcription level of hyaluronan synthases, receptors and mtDNA content |
title_full_unstemmed |
The effect of bovine embryo culture without proteins supplements until day 4 on transcription level of hyaluronan synthases, receptors and mtDNA content |
title_sort |
effect of bovine embryo culture without proteins supplements until day 4 on transcription level of hyaluronan synthases, receptors and mtdna content |
publisher |
Cambridge University Press |
publishDate |
2010 |
url |
http://hdl.handle.net/20.500.12792/3290 http://hdl.handle.net/10261/290633 |
work_keys_str_mv |
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